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New insight into the residual inactivation of Microcystis aeruginosa by dielectric barrier discharge.

Li L, Zhang H, Huang Q - Sci Rep (2015)

Bottom Line: Our results showed that the numbers of both dead and apoptotic cells increased with DBD treatment delay time, and hydrogen peroxide produced by DBD was the main reason for the time-delayed inactivation effect.However, apart from the influence of hydrogen peroxide, the DBD-induced initial injures on the algal cells during the discharge period also played a considerable role in the inactivation of the DBD treated cells, as indicated by the measurement of intracellular reactive oxygen species (ROS) inside the algal cells.We therefore propose an effective approach to utilization of non-thermal plasma technique that makes good use of the residual inactivation effect to optimize the experimental conditions in terms of discharge time and delay time, so that more efficient treatment of cyanobacterial blooms can be achieved.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Ion Beam Bio-engineering, Institute of Biotechnology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, 230031.

ABSTRACT
We report the new insight into the dielectric barrier discharge (DBD) induced inactivation of Microcystis aeruginosa, the dominant algae which caused harmful cyanobacterial blooms in many developing countries. In contrast with the previous work, we employed flow cytometry to examine the algal cells, so that we could assess the dead and living cells with more accuracy, and distinguish an intermediate state of algal cells which were verified as apoptotic. Our results showed that the numbers of both dead and apoptotic cells increased with DBD treatment delay time, and hydrogen peroxide produced by DBD was the main reason for the time-delayed inactivation effect. However, apart from the influence of hydrogen peroxide, the DBD-induced initial injures on the algal cells during the discharge period also played a considerable role in the inactivation of the DBD treated cells, as indicated by the measurement of intracellular reactive oxygen species (ROS) inside the algal cells. We therefore propose an effective approach to utilization of non-thermal plasma technique that makes good use of the residual inactivation effect to optimize the experimental conditions in terms of discharge time and delay time, so that more efficient treatment of cyanobacterial blooms can be achieved.

No MeSH data available.


The schematic diagram for illustrating the inactivation effect and mechanism for the DBD treatment of algal cells.
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f8: The schematic diagram for illustrating the inactivation effect and mechanism for the DBD treatment of algal cells.

Mentions: In summary, we have scrutinized the DBD induced inactivation of M. aeruginosa by using the method of flow cytometry, which ensured sensitive and reliable assessment of algal status and population; with this we have distinguished and confirmed apoptotic cells caused by DBD plasma discharge, and unambiguously discriminated the residual effect apart from the direct inactivation effect. We have also confirmed that the residual effect is mainly due to the remaining H2O2 generated by DBD process, but the initial damage to the algal cells during the discharge also elicits intracellular ROS which can make a considerable contribution to the residual inactivation effect partly through the apoptosis mechanism. A schematic plot for explaining the inactivation effect by DBD treatment is illustrated in Fig. 8. Our focus on the residual inactivation effect in this work may therefore not only provide a better understanding of the DBD induced inactivation mechanism, but also facilitate us to optimize discharge conditions such as plasma treatment time and delay time, so as to further improve the DBD treatment efficiency and gain better control of algal blooms.


New insight into the residual inactivation of Microcystis aeruginosa by dielectric barrier discharge.

Li L, Zhang H, Huang Q - Sci Rep (2015)

The schematic diagram for illustrating the inactivation effect and mechanism for the DBD treatment of algal cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561898&req=5

f8: The schematic diagram for illustrating the inactivation effect and mechanism for the DBD treatment of algal cells.
Mentions: In summary, we have scrutinized the DBD induced inactivation of M. aeruginosa by using the method of flow cytometry, which ensured sensitive and reliable assessment of algal status and population; with this we have distinguished and confirmed apoptotic cells caused by DBD plasma discharge, and unambiguously discriminated the residual effect apart from the direct inactivation effect. We have also confirmed that the residual effect is mainly due to the remaining H2O2 generated by DBD process, but the initial damage to the algal cells during the discharge also elicits intracellular ROS which can make a considerable contribution to the residual inactivation effect partly through the apoptosis mechanism. A schematic plot for explaining the inactivation effect by DBD treatment is illustrated in Fig. 8. Our focus on the residual inactivation effect in this work may therefore not only provide a better understanding of the DBD induced inactivation mechanism, but also facilitate us to optimize discharge conditions such as plasma treatment time and delay time, so as to further improve the DBD treatment efficiency and gain better control of algal blooms.

Bottom Line: Our results showed that the numbers of both dead and apoptotic cells increased with DBD treatment delay time, and hydrogen peroxide produced by DBD was the main reason for the time-delayed inactivation effect.However, apart from the influence of hydrogen peroxide, the DBD-induced initial injures on the algal cells during the discharge period also played a considerable role in the inactivation of the DBD treated cells, as indicated by the measurement of intracellular reactive oxygen species (ROS) inside the algal cells.We therefore propose an effective approach to utilization of non-thermal plasma technique that makes good use of the residual inactivation effect to optimize the experimental conditions in terms of discharge time and delay time, so that more efficient treatment of cyanobacterial blooms can be achieved.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Ion Beam Bio-engineering, Institute of Biotechnology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, 230031.

ABSTRACT
We report the new insight into the dielectric barrier discharge (DBD) induced inactivation of Microcystis aeruginosa, the dominant algae which caused harmful cyanobacterial blooms in many developing countries. In contrast with the previous work, we employed flow cytometry to examine the algal cells, so that we could assess the dead and living cells with more accuracy, and distinguish an intermediate state of algal cells which were verified as apoptotic. Our results showed that the numbers of both dead and apoptotic cells increased with DBD treatment delay time, and hydrogen peroxide produced by DBD was the main reason for the time-delayed inactivation effect. However, apart from the influence of hydrogen peroxide, the DBD-induced initial injures on the algal cells during the discharge period also played a considerable role in the inactivation of the DBD treated cells, as indicated by the measurement of intracellular reactive oxygen species (ROS) inside the algal cells. We therefore propose an effective approach to utilization of non-thermal plasma technique that makes good use of the residual inactivation effect to optimize the experimental conditions in terms of discharge time and delay time, so that more efficient treatment of cyanobacterial blooms can be achieved.

No MeSH data available.