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Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus

Neighbor-joining tree of candidate OBP proteins from Lepidoptera species.The protein names and sequences of the 193 OBPs that were used in this analysis are listed in Supplementary Table 8.
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f7: Neighbor-joining tree of candidate OBP proteins from Lepidoptera species.The protein names and sequences of the 193 OBPs that were used in this analysis are listed in Supplementary Table 8.

Mentions: A neighbor-joining tree of 193 OBP sequences was built from six different Lepidoptera species, including B. mori, A. ipsilon, H. armigera and the three closely related species of genus Spodoptera (S. litura, S. littoralis and S. exigua) (Fig. 7). It was shown that Lepidoptera OBPs can be divided into several distinct families, including the GOBP family, the PBP family, the Minus-C OBP family and the Plus-C OBP Family. The identified SlitPBP1-3 are clustered into the PBP family and the SlitGOBP1-2 are clustered into the GOBP family, respectively. In the PBP family, the PBP1, PBP2 and PBP3 from the genus Spodoptera (S. litura, S. littoralis and S. exigua) are each located in the same branch with the bootstrap values as high as 100. In the GOBP family, the GOBP1 and GOBP2 from the genus Spodoptera are each located in the same branch with the bootstrap values as high as 95. Three OBPs (SlitOBP11, SlitOBP12 and SlitOBP33) are clustered into the insect Minus-C OBP family, four OBPs (SlitOBP13, SlitOBP16, SlitOBP18 and SlitOBP27) are clustered into the insect Plus-C OBP family. The remaining 26 SlitOBPs are located in the same branch with corresponding homologous OBPs from the S. littoralis and S. exigua (Fig. 7). We found no species specific expansion and few numbers of gene duplication (SlitOBP14 and SlitOBP25).


Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Neighbor-joining tree of candidate OBP proteins from Lepidoptera species.The protein names and sequences of the 193 OBPs that were used in this analysis are listed in Supplementary Table 8.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561897&req=5

f7: Neighbor-joining tree of candidate OBP proteins from Lepidoptera species.The protein names and sequences of the 193 OBPs that were used in this analysis are listed in Supplementary Table 8.
Mentions: A neighbor-joining tree of 193 OBP sequences was built from six different Lepidoptera species, including B. mori, A. ipsilon, H. armigera and the three closely related species of genus Spodoptera (S. litura, S. littoralis and S. exigua) (Fig. 7). It was shown that Lepidoptera OBPs can be divided into several distinct families, including the GOBP family, the PBP family, the Minus-C OBP family and the Plus-C OBP Family. The identified SlitPBP1-3 are clustered into the PBP family and the SlitGOBP1-2 are clustered into the GOBP family, respectively. In the PBP family, the PBP1, PBP2 and PBP3 from the genus Spodoptera (S. litura, S. littoralis and S. exigua) are each located in the same branch with the bootstrap values as high as 100. In the GOBP family, the GOBP1 and GOBP2 from the genus Spodoptera are each located in the same branch with the bootstrap values as high as 95. Three OBPs (SlitOBP11, SlitOBP12 and SlitOBP33) are clustered into the insect Minus-C OBP family, four OBPs (SlitOBP13, SlitOBP16, SlitOBP18 and SlitOBP27) are clustered into the insect Plus-C OBP family. The remaining 26 SlitOBPs are located in the same branch with corresponding homologous OBPs from the S. littoralis and S. exigua (Fig. 7). We found no species specific expansion and few numbers of gene duplication (SlitOBP14 and SlitOBP25).

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus