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Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus

Motif analysis of Lepidoptera OBPs and CSPs.Parameters used for motif discovery were: minimum width = 6, maximum width = 10, maximum number of motif to find = 8. The upper parts in (A,B) listed the eight motifs discovered in the Lepidoptera OBPs and CSPs, receptively. All the motifs were discovered using MEME63 (version 4.9.1) on line server (http://meme.nbcr.net/meme/). The lower parts indicate approximate locations of each motif on the protein sequence. The numbers in the boxes correspond to the numbered motifs in the upper part of the figure, where small number indicates high conservation. The numbers on the bottom showed the approximate locations of each motif on the protein sequence, starting from the N-terminal. The protein names and sequences of the 384 OBPs and 225 CSPs from 36 different Lepidoptera species were listed in Supplementary Table 5.
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f6: Motif analysis of Lepidoptera OBPs and CSPs.Parameters used for motif discovery were: minimum width = 6, maximum width = 10, maximum number of motif to find = 8. The upper parts in (A,B) listed the eight motifs discovered in the Lepidoptera OBPs and CSPs, receptively. All the motifs were discovered using MEME63 (version 4.9.1) on line server (http://meme.nbcr.net/meme/). The lower parts indicate approximate locations of each motif on the protein sequence. The numbers in the boxes correspond to the numbered motifs in the upper part of the figure, where small number indicates high conservation. The numbers on the bottom showed the approximate locations of each motif on the protein sequence, starting from the N-terminal. The protein names and sequences of the 384 OBPs and 225 CSPs from 36 different Lepidoptera species were listed in Supplementary Table 5.

Mentions: When compared the motif-patterns of 384 OBPs from 36 Lepidoptera species we found 17 different motif-patterns, and 274 OBPs (71.4%) had the most common five motif-patterns, with 99 OBPs had the same motif-pattern as 8-6-2-1-4-5-3-7, thirty-six OBPs had the same motif-pattern as 8-6-2-1-4-5-3, forty-five OBPs had only two motifs with the order as 6-3, fifty-one OBPs had three motifs with the order as 6-1-3, forty-three OBPs only had one motif motif 6 (Fig. 6A). The remaining 61 OBPs shared the other 12 motif-patterns and none motif was found in 49 OBPs. While the motif-patterns of the 225 Lepidoptera CSPs were more conserved than the OBPs, 142 CSPs (63.1%) had the most common two motif-patterns, with 101 CSPs had motif-pattern as 8-4-5-1-6-2-7-3, and 41 CSPs had motif-pattern as 8-4-5-1-6-2-3. The 41 CSPs lost motif 7 compared with the 101 CSPs (Fig. 6B). The remaining 83 CSPs shared other 25 different motif-pattern. It should be noticed that the motif-patterns discovered by MEME in Figs 5 and 6 are not comparable, because different sets of sequences were used in each analysis.


Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Motif analysis of Lepidoptera OBPs and CSPs.Parameters used for motif discovery were: minimum width = 6, maximum width = 10, maximum number of motif to find = 8. The upper parts in (A,B) listed the eight motifs discovered in the Lepidoptera OBPs and CSPs, receptively. All the motifs were discovered using MEME63 (version 4.9.1) on line server (http://meme.nbcr.net/meme/). The lower parts indicate approximate locations of each motif on the protein sequence. The numbers in the boxes correspond to the numbered motifs in the upper part of the figure, where small number indicates high conservation. The numbers on the bottom showed the approximate locations of each motif on the protein sequence, starting from the N-terminal. The protein names and sequences of the 384 OBPs and 225 CSPs from 36 different Lepidoptera species were listed in Supplementary Table 5.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561897&req=5

f6: Motif analysis of Lepidoptera OBPs and CSPs.Parameters used for motif discovery were: minimum width = 6, maximum width = 10, maximum number of motif to find = 8. The upper parts in (A,B) listed the eight motifs discovered in the Lepidoptera OBPs and CSPs, receptively. All the motifs were discovered using MEME63 (version 4.9.1) on line server (http://meme.nbcr.net/meme/). The lower parts indicate approximate locations of each motif on the protein sequence. The numbers in the boxes correspond to the numbered motifs in the upper part of the figure, where small number indicates high conservation. The numbers on the bottom showed the approximate locations of each motif on the protein sequence, starting from the N-terminal. The protein names and sequences of the 384 OBPs and 225 CSPs from 36 different Lepidoptera species were listed in Supplementary Table 5.
Mentions: When compared the motif-patterns of 384 OBPs from 36 Lepidoptera species we found 17 different motif-patterns, and 274 OBPs (71.4%) had the most common five motif-patterns, with 99 OBPs had the same motif-pattern as 8-6-2-1-4-5-3-7, thirty-six OBPs had the same motif-pattern as 8-6-2-1-4-5-3, forty-five OBPs had only two motifs with the order as 6-3, fifty-one OBPs had three motifs with the order as 6-1-3, forty-three OBPs only had one motif motif 6 (Fig. 6A). The remaining 61 OBPs shared the other 12 motif-patterns and none motif was found in 49 OBPs. While the motif-patterns of the 225 Lepidoptera CSPs were more conserved than the OBPs, 142 CSPs (63.1%) had the most common two motif-patterns, with 101 CSPs had motif-pattern as 8-4-5-1-6-2-7-3, and 41 CSPs had motif-pattern as 8-4-5-1-6-2-3. The 41 CSPs lost motif 7 compared with the 101 CSPs (Fig. 6B). The remaining 83 CSPs shared other 25 different motif-pattern. It should be noticed that the motif-patterns discovered by MEME in Figs 5 and 6 are not comparable, because different sets of sequences were used in each analysis.

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus