Limits...
Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus

Motif analysis of OBPs in the genus Spodoptera.Parameters used for motif discovery were: minimum width = 6, maximum width = 10, maximum number of motif to find = 8. The upper parts listed the eight motifs discovered in the 78 OBPs using MEME63 (version 4.9.1) on line server (http://meme.nbcr.net/meme/). The lower parts indicate approximate locations of each motif on the protein sequence. The numbers in the boxes correspond to the numbered motifs in the upper part of the figure, where small number indicates high conservation. The numbers on the bottom showed the approximate locations of each motif on the protein sequence, starting from the N-terminal. This figure just listed the most common 10 motif-patterns which presented in 66 OBPs, with each motif-pattern present in more than two OBPs, the remaining 12 OBPs had 8 different motif-patterns with each of them presented in less than three OBPs. The protein names and sequences of the 78 of OBPs from S. litura, S. littoralis and S. exigua were listed in Supplementary Table 4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4561897&req=5

f5: Motif analysis of OBPs in the genus Spodoptera.Parameters used for motif discovery were: minimum width = 6, maximum width = 10, maximum number of motif to find = 8. The upper parts listed the eight motifs discovered in the 78 OBPs using MEME63 (version 4.9.1) on line server (http://meme.nbcr.net/meme/). The lower parts indicate approximate locations of each motif on the protein sequence. The numbers in the boxes correspond to the numbered motifs in the upper part of the figure, where small number indicates high conservation. The numbers on the bottom showed the approximate locations of each motif on the protein sequence, starting from the N-terminal. This figure just listed the most common 10 motif-patterns which presented in 66 OBPs, with each motif-pattern present in more than two OBPs, the remaining 12 OBPs had 8 different motif-patterns with each of them presented in less than three OBPs. The protein names and sequences of the 78 of OBPs from S. litura, S. littoralis and S. exigua were listed in Supplementary Table 4.

Mentions: The conserved motifs are important elements of functional domains. In order to compare the motif-pattern of OBP proteins in the genus Spodoptera, a total of 78 OBPs with intact ORFs from S. litura (31 OBPs), S. littoralis (30 OBPs) and S. exigua (17 OBPs) were combined into one set of sequences and then submitted to the MEME server to discover the conserved motifs. The results indicated that eight motifs were found and the OBPs in each species had different motif-patterns, but the homologous OBPs among S. litura, S. littoralis and S. exigua had similar motif-patterns (Fig. 5). Eighteen different motif-patterns were presented in the tested 78 OBPs, in Fig. 5 we just listed and analysis the most common 10 motif-patterns which presented in 66 OBPs, with each motif-pattern present in more than two OBPs, the remaining 12 OBPs had 8 different motif-patterns with each of them presented in less than three OBPs. The three PBPs of the three species had the same motif-pattern with the motif order as 4-1-5-8-2, the GOBP1/2 of the three species had one more motif 3 at the N-terminal compared with PBP1/2/3, with the motif order as 3-4-1-5-8-2. Twelve homologous OBPs (SlitOBP3/7/11/17, SlittoOBP3/14/17/18/20 and SexiOBP2/5/10) had four motifs with an order as 3-1-7-2; sixteen homologous OBPs (SlitOBP5/6/12/22/23/28, SlittoOBP11/12/23/24/25/28, SexiABP1 and SexiOBP1/3/4) had five motifs with the order as 3-1-7-6-2 (Fig. 5). We also found some interesting regular patterns as follows in Fig. 5: the motif 2 and motif 1 existed in 61 out of 66 OBPs and located at same position (motif 2 at the C-terminal and motif 1 in the middle) with the exception of SlitOBP16/18/27 and SlittoOBP1/16 that did not have motif 1. The motif 4, 5 and 8 were only found in the PBP1/2/3 and GOBP1/2 in the three species and located in the N-terminal, central part and the C-terminal, respectively. Interestingly GOBPs had motif 3 which is common for OBPs and motif 4-1-5-8-2 which is common for PBPs. The motif 7 was only found in OBPs but in PBP/GOBP complex.


Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Motif analysis of OBPs in the genus Spodoptera.Parameters used for motif discovery were: minimum width = 6, maximum width = 10, maximum number of motif to find = 8. The upper parts listed the eight motifs discovered in the 78 OBPs using MEME63 (version 4.9.1) on line server (http://meme.nbcr.net/meme/). The lower parts indicate approximate locations of each motif on the protein sequence. The numbers in the boxes correspond to the numbered motifs in the upper part of the figure, where small number indicates high conservation. The numbers on the bottom showed the approximate locations of each motif on the protein sequence, starting from the N-terminal. This figure just listed the most common 10 motif-patterns which presented in 66 OBPs, with each motif-pattern present in more than two OBPs, the remaining 12 OBPs had 8 different motif-patterns with each of them presented in less than three OBPs. The protein names and sequences of the 78 of OBPs from S. litura, S. littoralis and S. exigua were listed in Supplementary Table 4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561897&req=5

f5: Motif analysis of OBPs in the genus Spodoptera.Parameters used for motif discovery were: minimum width = 6, maximum width = 10, maximum number of motif to find = 8. The upper parts listed the eight motifs discovered in the 78 OBPs using MEME63 (version 4.9.1) on line server (http://meme.nbcr.net/meme/). The lower parts indicate approximate locations of each motif on the protein sequence. The numbers in the boxes correspond to the numbered motifs in the upper part of the figure, where small number indicates high conservation. The numbers on the bottom showed the approximate locations of each motif on the protein sequence, starting from the N-terminal. This figure just listed the most common 10 motif-patterns which presented in 66 OBPs, with each motif-pattern present in more than two OBPs, the remaining 12 OBPs had 8 different motif-patterns with each of them presented in less than three OBPs. The protein names and sequences of the 78 of OBPs from S. litura, S. littoralis and S. exigua were listed in Supplementary Table 4.
Mentions: The conserved motifs are important elements of functional domains. In order to compare the motif-pattern of OBP proteins in the genus Spodoptera, a total of 78 OBPs with intact ORFs from S. litura (31 OBPs), S. littoralis (30 OBPs) and S. exigua (17 OBPs) were combined into one set of sequences and then submitted to the MEME server to discover the conserved motifs. The results indicated that eight motifs were found and the OBPs in each species had different motif-patterns, but the homologous OBPs among S. litura, S. littoralis and S. exigua had similar motif-patterns (Fig. 5). Eighteen different motif-patterns were presented in the tested 78 OBPs, in Fig. 5 we just listed and analysis the most common 10 motif-patterns which presented in 66 OBPs, with each motif-pattern present in more than two OBPs, the remaining 12 OBPs had 8 different motif-patterns with each of them presented in less than three OBPs. The three PBPs of the three species had the same motif-pattern with the motif order as 4-1-5-8-2, the GOBP1/2 of the three species had one more motif 3 at the N-terminal compared with PBP1/2/3, with the motif order as 3-4-1-5-8-2. Twelve homologous OBPs (SlitOBP3/7/11/17, SlittoOBP3/14/17/18/20 and SexiOBP2/5/10) had four motifs with an order as 3-1-7-2; sixteen homologous OBPs (SlitOBP5/6/12/22/23/28, SlittoOBP11/12/23/24/25/28, SexiABP1 and SexiOBP1/3/4) had five motifs with the order as 3-1-7-6-2 (Fig. 5). We also found some interesting regular patterns as follows in Fig. 5: the motif 2 and motif 1 existed in 61 out of 66 OBPs and located at same position (motif 2 at the C-terminal and motif 1 in the middle) with the exception of SlitOBP16/18/27 and SlittoOBP1/16 that did not have motif 1. The motif 4, 5 and 8 were only found in the PBP1/2/3 and GOBP1/2 in the three species and located in the N-terminal, central part and the C-terminal, respectively. Interestingly GOBPs had motif 3 which is common for OBPs and motif 4-1-5-8-2 which is common for PBPs. The motif 7 was only found in OBPs but in PBP/GOBP complex.

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.


Related in: MedlinePlus