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Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.


Alignment of the S. litura OBPs.Full-length amino acid sequences of S. litura OBPs are aligned by ClustalX 2.1. Green boxes show conserved cysteine residues. Accession numbers of the S. litura OBPs are listed in Table 2.
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f4: Alignment of the S. litura OBPs.Full-length amino acid sequences of S. litura OBPs are aligned by ClustalX 2.1. Green boxes show conserved cysteine residues. Accession numbers of the S. litura OBPs are listed in Table 2.

Mentions: We identified 38 putative OBP genes in the S. litura 454 and Hiseq2000 antennal transcriptome data. These include three pheromone binding proteins (PBPs) and two general odorant binding proteins (GOBPs) (Table 2). Thirty of the 38 OBP genes (except SlitOBP1, SlitOBP2, SlitOBP14, SlitOBP24, SlitOBP26, SlitOBP30-32) have intact ORFs with lengths ranging from 387 bp to 1017 bp, all the full-length OBPs have a signal peptide at their N-terminal, a signature of secretory proteins (Table 2). Based on the number and location of the conserved cysteines, the 30 full-length SlitOBPs can be divided into three families: SlitOBP11, SlitOBP12 and SlitOBP33 belong to the Minus-C OBP family, which have no conserved cysteines C2 and C5 (Fig. 4); SlitOBP13, SlitOBP16, SlitOBP18 and SlitOBP27 belong to the Plus-C OBP family, which have additional 2, 3, 6 and 2 cysteines located downstream of conserved C6 in addition to the six conserved cysteines. Furthermore, the conserved C2 and C3 of the four Plus-C OBPs are separated by 4 amino acid residues rather than usual 3 of the Classic OBP, and the conserved C5 and C6 of three Plus-C OBPs (SlitOBP16, SlitOBP18 and SlitOBP27) are separated by 7 amino acid residues rather than usual 8 as in the Plus-C OBP SlitOBP13 and the non-Plus-C SlitOBPs (Fig. 4); The remaining 23 SlitOBPs belong to the Classic OBP family, which all having typical six conserved cysteines and spacing between them (Fig. 4).


Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Alignment of the S. litura OBPs.Full-length amino acid sequences of S. litura OBPs are aligned by ClustalX 2.1. Green boxes show conserved cysteine residues. Accession numbers of the S. litura OBPs are listed in Table 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561897&req=5

f4: Alignment of the S. litura OBPs.Full-length amino acid sequences of S. litura OBPs are aligned by ClustalX 2.1. Green boxes show conserved cysteine residues. Accession numbers of the S. litura OBPs are listed in Table 2.
Mentions: We identified 38 putative OBP genes in the S. litura 454 and Hiseq2000 antennal transcriptome data. These include three pheromone binding proteins (PBPs) and two general odorant binding proteins (GOBPs) (Table 2). Thirty of the 38 OBP genes (except SlitOBP1, SlitOBP2, SlitOBP14, SlitOBP24, SlitOBP26, SlitOBP30-32) have intact ORFs with lengths ranging from 387 bp to 1017 bp, all the full-length OBPs have a signal peptide at their N-terminal, a signature of secretory proteins (Table 2). Based on the number and location of the conserved cysteines, the 30 full-length SlitOBPs can be divided into three families: SlitOBP11, SlitOBP12 and SlitOBP33 belong to the Minus-C OBP family, which have no conserved cysteines C2 and C5 (Fig. 4); SlitOBP13, SlitOBP16, SlitOBP18 and SlitOBP27 belong to the Plus-C OBP family, which have additional 2, 3, 6 and 2 cysteines located downstream of conserved C6 in addition to the six conserved cysteines. Furthermore, the conserved C2 and C3 of the four Plus-C OBPs are separated by 4 amino acid residues rather than usual 3 of the Classic OBP, and the conserved C5 and C6 of three Plus-C OBPs (SlitOBP16, SlitOBP18 and SlitOBP27) are separated by 7 amino acid residues rather than usual 8 as in the Plus-C OBP SlitOBP13 and the non-Plus-C SlitOBPs (Fig. 4); The remaining 23 SlitOBPs belong to the Classic OBP family, which all having typical six conserved cysteines and spacing between them (Fig. 4).

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.