Limits...
Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.


Top 10 best hits of the BLASTn results.All S. litura antennal unigenes were used in BLASTn search the GenBank entries. The best hits with an E-value < = 1.0E-5 for each query was grouped according to species.
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f2: Top 10 best hits of the BLASTn results.All S. litura antennal unigenes were used in BLASTn search the GenBank entries. The best hits with an E-value < = 1.0E-5 for each query was grouped according to species.

Mentions: Homology searching of the 21223 S. litura antennal unigenes with other insect species was conducted using the BLASTx and BLASTn programs with the E-value cut-off of 10e-533. The results indicated that 10438 of the 21223 unigenes (49.2%) had BLASTx hits in the non-redundant protein (nr) databases and 8957 unigenes (42.2%) had BLASTn hits in the non-redundant nucleotide sequence (nt) databases. Most annotated S. litura antennal unigenes had the highest hits with the Lepidoptera insect genes (5173 of the 8957 nt-hit unigenes); the highest number of hits include 1235 unigenes that were homologous to Spodoptera frugiperda genes, 934 unigenes that were homologous to Bombyx mori genes, 910 and 834 unigenes that were homologous to S. litura and S. littoralis genes, respectively. The second highest number of hits were with the Dipteran species genes, with 457 hits of the D. melanogaster genes, and 352 and 287 hits that were homologous to genes of the mosquitoes Culex quinquefasciatus and Anopheles gambiae, respectively (Fig. 2).


Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura.

Gu SH, Zhou JJ, Gao S, Wang DH, Li XC, Guo YY, Zhang YJ - Sci Rep (2015)

Top 10 best hits of the BLASTn results.All S. litura antennal unigenes were used in BLASTn search the GenBank entries. The best hits with an E-value < = 1.0E-5 for each query was grouped according to species.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561897&req=5

f2: Top 10 best hits of the BLASTn results.All S. litura antennal unigenes were used in BLASTn search the GenBank entries. The best hits with an E-value < = 1.0E-5 for each query was grouped according to species.
Mentions: Homology searching of the 21223 S. litura antennal unigenes with other insect species was conducted using the BLASTx and BLASTn programs with the E-value cut-off of 10e-533. The results indicated that 10438 of the 21223 unigenes (49.2%) had BLASTx hits in the non-redundant protein (nr) databases and 8957 unigenes (42.2%) had BLASTn hits in the non-redundant nucleotide sequence (nt) databases. Most annotated S. litura antennal unigenes had the highest hits with the Lepidoptera insect genes (5173 of the 8957 nt-hit unigenes); the highest number of hits include 1235 unigenes that were homologous to Spodoptera frugiperda genes, 934 unigenes that were homologous to Bombyx mori genes, 910 and 834 unigenes that were homologous to S. litura and S. littoralis genes, respectively. The second highest number of hits were with the Dipteran species genes, with 457 hits of the D. melanogaster genes, and 352 and 287 hits that were homologous to genes of the mosquitoes Culex quinquefasciatus and Anopheles gambiae, respectively (Fig. 2).

Bottom Line: Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here.The motif-patterns between Lepidoptera OBPs and CSPs are also compared.The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

No MeSH data available.