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Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus

Effect on P. vulgaris emergence of treatments using the commercial product (Tebuzin 250 SC) and the nanoparticle formulations at different fungicide concentrations.(A) Green mass of the aerial part, 15 days after germination; (B) green mass of the root part, 15 days after germination; (C) dry mass of the aerial part after drying for 7 days; (D) dry mass of the root part after drying for 7 days. The level of significance used was p < 0.05 for the differences observed between the groups, where significant differences are indicated as follows: a* (compared to the control); b* (compared to 0.7 mg/mL); c* (compared to 0.5 mg/mL); d* (compared to 0.2 mg/mL); and e* (compared to 0.1 mg/mL).
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f9: Effect on P. vulgaris emergence of treatments using the commercial product (Tebuzin 250 SC) and the nanoparticle formulations at different fungicide concentrations.(A) Green mass of the aerial part, 15 days after germination; (B) green mass of the root part, 15 days after germination; (C) dry mass of the aerial part after drying for 7 days; (D) dry mass of the root part after drying for 7 days. The level of significance used was p < 0.05 for the differences observed between the groups, where significant differences are indicated as follows: a* (compared to the control); b* (compared to 0.7 mg/mL); c* (compared to 0.5 mg/mL); d* (compared to 0.2 mg/mL); and e* (compared to 0.1 mg/mL).

Mentions: The influence of the commercial and nanoparticle formulations on plant emergence was evaluated using P. vulgaris seeds. The germination index of the seeds (92%) was not affected by the commercial product or by the nanoparticle formulations (with or without fungicides). However, in the case of emergence, there was a progressive decrease in the fresh weights of roots and shoots when the concentrations of the fungicides were increased (Fig. 9). The fresh weight of the aerial and root parts of the seedlings were analyzed for 15 days after emergence, comparing plants treated using the different formulations with untreated plants. There was a progressive reduction in the biomass of the aerial and root parts when the concentration of the fungicides was increased (Fig. 9A,B). Smaller effects were observed using the PCL nanocapsules containing the two fungicides, compared to the commercial product and the SLN formulation. This could be explained by the more gradual release of the fungicides from the interior of the nanocapsules. The greatest effect on the emergence was observed for the loaded SLNs, for which plant growth was lower than for both the control and the commercial formulation. The results therefore indicated that in terms of fresh plant mass, the loaded NCs caused least impact on plant development, relative to the control. Similar trends were observed for the dry masses of the aerial and root parts of the plants.


Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Effect on P. vulgaris emergence of treatments using the commercial product (Tebuzin 250 SC) and the nanoparticle formulations at different fungicide concentrations.(A) Green mass of the aerial part, 15 days after germination; (B) green mass of the root part, 15 days after germination; (C) dry mass of the aerial part after drying for 7 days; (D) dry mass of the root part after drying for 7 days. The level of significance used was p < 0.05 for the differences observed between the groups, where significant differences are indicated as follows: a* (compared to the control); b* (compared to 0.7 mg/mL); c* (compared to 0.5 mg/mL); d* (compared to 0.2 mg/mL); and e* (compared to 0.1 mg/mL).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561881&req=5

f9: Effect on P. vulgaris emergence of treatments using the commercial product (Tebuzin 250 SC) and the nanoparticle formulations at different fungicide concentrations.(A) Green mass of the aerial part, 15 days after germination; (B) green mass of the root part, 15 days after germination; (C) dry mass of the aerial part after drying for 7 days; (D) dry mass of the root part after drying for 7 days. The level of significance used was p < 0.05 for the differences observed between the groups, where significant differences are indicated as follows: a* (compared to the control); b* (compared to 0.7 mg/mL); c* (compared to 0.5 mg/mL); d* (compared to 0.2 mg/mL); and e* (compared to 0.1 mg/mL).
Mentions: The influence of the commercial and nanoparticle formulations on plant emergence was evaluated using P. vulgaris seeds. The germination index of the seeds (92%) was not affected by the commercial product or by the nanoparticle formulations (with or without fungicides). However, in the case of emergence, there was a progressive decrease in the fresh weights of roots and shoots when the concentrations of the fungicides were increased (Fig. 9). The fresh weight of the aerial and root parts of the seedlings were analyzed for 15 days after emergence, comparing plants treated using the different formulations with untreated plants. There was a progressive reduction in the biomass of the aerial and root parts when the concentration of the fungicides was increased (Fig. 9A,B). Smaller effects were observed using the PCL nanocapsules containing the two fungicides, compared to the commercial product and the SLN formulation. This could be explained by the more gradual release of the fungicides from the interior of the nanocapsules. The greatest effect on the emergence was observed for the loaded SLNs, for which plant growth was lower than for both the control and the commercial formulation. The results therefore indicated that in terms of fresh plant mass, the loaded NCs caused least impact on plant development, relative to the control. Similar trends were observed for the dry masses of the aerial and root parts of the plants.

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus