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Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity evaluation of the different formulations (NC_PCL, NC_MBC_TBZ, SLN, SLN_MBC:TBZ and Commercial Formulation).The fungicide concentrations were investigate in the range of 31.25 and 250 μg/mL. The assays were performed using Balb/c 3T3 mouse fibroblasts (A); MC-3T3 osteoblast (B) and HeLa adenocarcinoma (C) and the MTT reduction test (n = 3). NC_PCL and SLN were tested in the same dilution conditions from the systems containing fungicides.
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f5: Cytotoxicity evaluation of the different formulations (NC_PCL, NC_MBC_TBZ, SLN, SLN_MBC:TBZ and Commercial Formulation).The fungicide concentrations were investigate in the range of 31.25 and 250 μg/mL. The assays were performed using Balb/c 3T3 mouse fibroblasts (A); MC-3T3 osteoblast (B) and HeLa adenocarcinoma (C) and the MTT reduction test (n = 3). NC_PCL and SLN were tested in the same dilution conditions from the systems containing fungicides.

Mentions: Apart from the physio-chemical and morphological characterizations, the in vitro cellular viability test was conducted to assess the cytotoxicity of the solid lipid nanoparticles loading fungicides (Fig. 5). For this, there different cell types (two normal and one cancerous cell lines) such as preosteoblast (MC3T3-E1); adenocarcinoma (HeLa) and fibroblast (Balb/c 3T3) were employed to evaluate percentage cell viability under the presence of prepared nanoparticles and compared the viability with that of the commercial formulations. The results of the cellular viability assays obtained for normal cells (3T3 and MC3T3) clearly showed that independent of the cell types employed, the current nanoparticles were less toxic than the commercial fungicides (Fig. 5). This can be evidenced from the fact that the percentage cell viability in the presence of newly prepared nanoparticles was lower than that of the commercial product. Moreover, the experimental results indicates that the effect of exposure of the cells to the commercial formulation was dose-dependent, with cellular viability values below 25% and 60% for the cell lines 3T3 and MC-3T3 respectively at the highest concentration, which clearly revealed the cytotoxicity of the fungicides. However, the results obtained with the adenocarcinoma cells (HeLa) showed that the cytotoxicity of the solid lipid nanoparticles loading fungicides was higher than commercial formulation. This result can be attributed to the difference in uptake of these nanoparticles by the cells43. Furthermore, it is already known that carbendazim possesses the ability to inhibit the proliferation of human cancer cells4445.


Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Cytotoxicity evaluation of the different formulations (NC_PCL, NC_MBC_TBZ, SLN, SLN_MBC:TBZ and Commercial Formulation).The fungicide concentrations were investigate in the range of 31.25 and 250 μg/mL. The assays were performed using Balb/c 3T3 mouse fibroblasts (A); MC-3T3 osteoblast (B) and HeLa adenocarcinoma (C) and the MTT reduction test (n = 3). NC_PCL and SLN were tested in the same dilution conditions from the systems containing fungicides.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561881&req=5

f5: Cytotoxicity evaluation of the different formulations (NC_PCL, NC_MBC_TBZ, SLN, SLN_MBC:TBZ and Commercial Formulation).The fungicide concentrations were investigate in the range of 31.25 and 250 μg/mL. The assays were performed using Balb/c 3T3 mouse fibroblasts (A); MC-3T3 osteoblast (B) and HeLa adenocarcinoma (C) and the MTT reduction test (n = 3). NC_PCL and SLN were tested in the same dilution conditions from the systems containing fungicides.
Mentions: Apart from the physio-chemical and morphological characterizations, the in vitro cellular viability test was conducted to assess the cytotoxicity of the solid lipid nanoparticles loading fungicides (Fig. 5). For this, there different cell types (two normal and one cancerous cell lines) such as preosteoblast (MC3T3-E1); adenocarcinoma (HeLa) and fibroblast (Balb/c 3T3) were employed to evaluate percentage cell viability under the presence of prepared nanoparticles and compared the viability with that of the commercial formulations. The results of the cellular viability assays obtained for normal cells (3T3 and MC3T3) clearly showed that independent of the cell types employed, the current nanoparticles were less toxic than the commercial fungicides (Fig. 5). This can be evidenced from the fact that the percentage cell viability in the presence of newly prepared nanoparticles was lower than that of the commercial product. Moreover, the experimental results indicates that the effect of exposure of the cells to the commercial formulation was dose-dependent, with cellular viability values below 25% and 60% for the cell lines 3T3 and MC-3T3 respectively at the highest concentration, which clearly revealed the cytotoxicity of the fungicides. However, the results obtained with the adenocarcinoma cells (HeLa) showed that the cytotoxicity of the solid lipid nanoparticles loading fungicides was higher than commercial formulation. This result can be attributed to the difference in uptake of these nanoparticles by the cells43. Furthermore, it is already known that carbendazim possesses the ability to inhibit the proliferation of human cancer cells4445.

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus