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Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus

Morphological (TEM) and size distribution (PCS) analysis of the nanoparticles containing MBC and TBZ, performed immediately after preparation and then after 120 days of storage.(A) NC-PCL, using TEM magnifications of 27,800x and 15,600x (Day 0) and 129,300 and 21,560x (Day 120); (B) SLN, using TEM magnifications of 77,500x and 27,800x (Day 0) and 21,600x and 89,230x (Day 120).
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f4: Morphological (TEM) and size distribution (PCS) analysis of the nanoparticles containing MBC and TBZ, performed immediately after preparation and then after 120 days of storage.(A) NC-PCL, using TEM magnifications of 27,800x and 15,600x (Day 0) and 129,300 and 21,560x (Day 120); (B) SLN, using TEM magnifications of 77,500x and 27,800x (Day 0) and 21,600x and 89,230x (Day 120).

Mentions: Morphological characterization of the prepared nanoparticles was also done in the present study. For this, TEM was employed to investigate the morphology of the two types of nanoparticle. The NCs and SLNs were both spherical (Fig. 4) and the average particle sizes were 490 and 191.6 nm, respectively (Fig. 4A,B). The agglomerates visible in the micrographs resulted from the sample drying process. These sizes were smaller than those determined using photon correlation spectroscopy, which can be explained by the fact that the particles observed by TEM were dehydrated, while the PCS technique measured particles present in an aqueous medium.


Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Morphological (TEM) and size distribution (PCS) analysis of the nanoparticles containing MBC and TBZ, performed immediately after preparation and then after 120 days of storage.(A) NC-PCL, using TEM magnifications of 27,800x and 15,600x (Day 0) and 129,300 and 21,560x (Day 120); (B) SLN, using TEM magnifications of 77,500x and 27,800x (Day 0) and 21,600x and 89,230x (Day 120).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561881&req=5

f4: Morphological (TEM) and size distribution (PCS) analysis of the nanoparticles containing MBC and TBZ, performed immediately after preparation and then after 120 days of storage.(A) NC-PCL, using TEM magnifications of 27,800x and 15,600x (Day 0) and 129,300 and 21,560x (Day 120); (B) SLN, using TEM magnifications of 77,500x and 27,800x (Day 0) and 21,600x and 89,230x (Day 120).
Mentions: Morphological characterization of the prepared nanoparticles was also done in the present study. For this, TEM was employed to investigate the morphology of the two types of nanoparticle. The NCs and SLNs were both spherical (Fig. 4) and the average particle sizes were 490 and 191.6 nm, respectively (Fig. 4A,B). The agglomerates visible in the micrographs resulted from the sample drying process. These sizes were smaller than those determined using photon correlation spectroscopy, which can be explained by the fact that the particles observed by TEM were dehydrated, while the PCS technique measured particles present in an aqueous medium.

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus