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Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus

Evaluation of interaction between the fungicides and components of the PCL nanocapsule formulation.FTIR spectra for: (A) MBC, (B) TBZ, (C) PCL, (D) NC-PCL, (E) NC-MBC:TBZ. The arrows indicate the main characteristic absorption bands in each spectrum.
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f2: Evaluation of interaction between the fungicides and components of the PCL nanocapsule formulation.FTIR spectra for: (A) MBC, (B) TBZ, (C) PCL, (D) NC-PCL, (E) NC-MBC:TBZ. The arrows indicate the main characteristic absorption bands in each spectrum.

Mentions: In order to characterize these systems FTIR was used to identify possible interactions between the fungicides and the nanoparticles, as well as to determine whether the components of the formulations were altered in any way during the preparation process. Figures 2 and 3 show the spectra for the different samples. The spectrum for MBC (Fig. 2A) revealed characteristic bands including aromatic C=C stretching at 1595 cm−1, stretching of ester C=O at 1630 cm−1, and stretching of benzene ring C−H at 3056 cm−1. The spectrum for TBZ (Fig. 2B) showed stretching vibration of benzene ring C−H at 3042 cm−1, vibration of benzene ring C=C bonds between 1570 and 1511 cm−1, and a band at 3300 cm−1 related to the alcohol group of the TBZ molecule. The PCL polymer (Fig. 2C) showed characteristic bands including the stretching of ester carbonyls (C=O) at 1735 cm−1 and O−H at 3440 cm−1, as well as C−H stretching of saturated carbon present in the polymeric chains of PCL between 3000 and 2800 cm−1. The spectrum for the nanoparticles without fungicides (Fig. 2D) showed that the bands remained unaltered compared to the spectrum for the PCL polymer. However, in the case of the nanoparticles containing the fungicides (Fig. 2E), in addition to the characteristic bands of the PCL polymer, there was a band related to the stretching of C=C of the aromatic ring of carbendazim, which was not observed in the spectrum of the NCs alone. However, no band corresponding to C=C stretching of the aromatic ring of tebuconazole was observed, probably due to the overlap with the carbonyl band of the PCL polymer.


Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Evaluation of interaction between the fungicides and components of the PCL nanocapsule formulation.FTIR spectra for: (A) MBC, (B) TBZ, (C) PCL, (D) NC-PCL, (E) NC-MBC:TBZ. The arrows indicate the main characteristic absorption bands in each spectrum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561881&req=5

f2: Evaluation of interaction between the fungicides and components of the PCL nanocapsule formulation.FTIR spectra for: (A) MBC, (B) TBZ, (C) PCL, (D) NC-PCL, (E) NC-MBC:TBZ. The arrows indicate the main characteristic absorption bands in each spectrum.
Mentions: In order to characterize these systems FTIR was used to identify possible interactions between the fungicides and the nanoparticles, as well as to determine whether the components of the formulations were altered in any way during the preparation process. Figures 2 and 3 show the spectra for the different samples. The spectrum for MBC (Fig. 2A) revealed characteristic bands including aromatic C=C stretching at 1595 cm−1, stretching of ester C=O at 1630 cm−1, and stretching of benzene ring C−H at 3056 cm−1. The spectrum for TBZ (Fig. 2B) showed stretching vibration of benzene ring C−H at 3042 cm−1, vibration of benzene ring C=C bonds between 1570 and 1511 cm−1, and a band at 3300 cm−1 related to the alcohol group of the TBZ molecule. The PCL polymer (Fig. 2C) showed characteristic bands including the stretching of ester carbonyls (C=O) at 1735 cm−1 and O−H at 3440 cm−1, as well as C−H stretching of saturated carbon present in the polymeric chains of PCL between 3000 and 2800 cm−1. The spectrum for the nanoparticles without fungicides (Fig. 2D) showed that the bands remained unaltered compared to the spectrum for the PCL polymer. However, in the case of the nanoparticles containing the fungicides (Fig. 2E), in addition to the characteristic bands of the PCL polymer, there was a band related to the stretching of C=C of the aromatic ring of carbendazim, which was not observed in the spectrum of the NCs alone. However, no band corresponding to C=C stretching of the aromatic ring of tebuconazole was observed, probably due to the overlap with the carbonyl band of the PCL polymer.

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus