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Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus

Determination of nanoparticle stability: (A) nanoparticle diameter (z-average, nm); (B) polydispersivity index; (C) zeta potential (mV); (D) encapsulation efficiency.Measurements were made of the formulations containing the polymeric nanocapsules and solid lipid nanoparticles, with and without the fungicides, at ambient temperature after different periods of storage (0, 15, 30, 60, 90, and 120 days). The values shown represent the averages of three determinations. The level of significance was p < 0.05 for the statistical differences observed between the groups, where the a* represents statistical differences compared to 0 day; b* compared to 15 days; c* for the 30 days; d* for the 60 days and e* for the 90 days.
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f1: Determination of nanoparticle stability: (A) nanoparticle diameter (z-average, nm); (B) polydispersivity index; (C) zeta potential (mV); (D) encapsulation efficiency.Measurements were made of the formulations containing the polymeric nanocapsules and solid lipid nanoparticles, with and without the fungicides, at ambient temperature after different periods of storage (0, 15, 30, 60, 90, and 120 days). The values shown represent the averages of three determinations. The level of significance was p < 0.05 for the statistical differences observed between the groups, where the a* represents statistical differences compared to 0 day; b* compared to 15 days; c* for the 30 days; d* for the 60 days and e* for the 90 days.

Mentions: The stability of the formulations was evaluated using measurements of average diameter, polydispersivity index, zeta potential, and encapsulation efficiency. These are the essential parameters used to obtain information concerning the nature of colloidal systems. The analyses were performed immediately after preparation and then after 15, 30, 60, 90, and 120 days of storage at ambient temperature (25 °C). The results obtained are presented in Fig. 1.


Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications.

Campos EV, de Oliveira JL, da Silva CM, Pascoli M, Pasquoto T, Lima R, Abhilash PC, Fraceto LF - Sci Rep (2015)

Determination of nanoparticle stability: (A) nanoparticle diameter (z-average, nm); (B) polydispersivity index; (C) zeta potential (mV); (D) encapsulation efficiency.Measurements were made of the formulations containing the polymeric nanocapsules and solid lipid nanoparticles, with and without the fungicides, at ambient temperature after different periods of storage (0, 15, 30, 60, 90, and 120 days). The values shown represent the averages of three determinations. The level of significance was p < 0.05 for the statistical differences observed between the groups, where the a* represents statistical differences compared to 0 day; b* compared to 15 days; c* for the 30 days; d* for the 60 days and e* for the 90 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561881&req=5

f1: Determination of nanoparticle stability: (A) nanoparticle diameter (z-average, nm); (B) polydispersivity index; (C) zeta potential (mV); (D) encapsulation efficiency.Measurements were made of the formulations containing the polymeric nanocapsules and solid lipid nanoparticles, with and without the fungicides, at ambient temperature after different periods of storage (0, 15, 30, 60, 90, and 120 days). The values shown represent the averages of three determinations. The level of significance was p < 0.05 for the statistical differences observed between the groups, where the a* represents statistical differences compared to 0 day; b* compared to 15 days; c* for the 30 days; d* for the 60 days and e* for the 90 days.
Mentions: The stability of the formulations was evaluated using measurements of average diameter, polydispersivity index, zeta potential, and encapsulation efficiency. These are the essential parameters used to obtain information concerning the nature of colloidal systems. The analyses were performed immediately after preparation and then after 15, 30, 60, 90, and 120 days of storage at ambient temperature (25 °C). The results obtained are presented in Fig. 1.

Bottom Line: Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles.The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity.These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Engineering, State University of São Paulo (UNESP), Sorocaba, SP, Brazil.

ABSTRACT
Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

No MeSH data available.


Related in: MedlinePlus