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Effects of the Pinggan Qianyang Recipe on MicroRNA Gene Expression in the Aortic Tissue of Spontaneously Hypertensive Rats.

Zhong G, Fang X, Wang D, Chen Q, Tang T - Evid Based Complement Alternat Med (2015)

Bottom Line: Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups.These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process.The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

View Article: PubMed Central - PubMed

Affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The present study aimed to investigate the relationship between miRNAs and in spontaneously hypertensive rats (SHR) vascular remodeling and analyze the impact of the Pinggan Qianyang recipe (PQR) on miRNAs. Mammalian miRNA microarrays containing 509 miRNA genes were employed to analyze the differentially expressed miRNAs in the three groups. MiRNAs were considered to be up- or downregulated when the fluorescent intensity ratio between the two groups was over 4-fold. Validation of those miRNAs changed in SHR after PQR treatment was used by real-time quantitative RT-PCR (qRT-PCR). Compared with the normal group, a total of 32 miRNAs were differentially expressed by more than twofold; among these, 18 were upregulated and 14 were downregulated in the model group. Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups. These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process. The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

No MeSH data available.


Validation of miRNA microarray data by qRT-PCR. (a) miR-20a; (b) miR-145; (c) miRNA-98; (d) miR-30. The relative expression of four miRNAs was normalized to the expression of the internal control gene (U6).
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fig5: Validation of miRNA microarray data by qRT-PCR. (a) miR-20a; (b) miR-145; (c) miRNA-98; (d) miR-30. The relative expression of four miRNAs was normalized to the expression of the internal control gene (U6).

Mentions: qRT-PCR is a quantitative and specific method that can be used to distinguish a single nucleotide difference between miRNAs. Thus, involution was obtained by miChip analysis for four selected miRNAs that showed either high (miR-145) or low (miR-30) signal intensities, or high (miR-20a) or low (miRNA-98) differential expression values among the three groups. The results of qRT-PCR analysis were often more reliable than those of the microarray analysis. qRT-PCR showed that miR-145 and miR-20a expression was downregulated in the model group compared with their expression in the PQR group, which was consistent with the microarray results. Thus, the miRNA expression profiles obtained by qRT-PCR fully support the results of miChip analysis (Figure 5).


Effects of the Pinggan Qianyang Recipe on MicroRNA Gene Expression in the Aortic Tissue of Spontaneously Hypertensive Rats.

Zhong G, Fang X, Wang D, Chen Q, Tang T - Evid Based Complement Alternat Med (2015)

Validation of miRNA microarray data by qRT-PCR. (a) miR-20a; (b) miR-145; (c) miRNA-98; (d) miR-30. The relative expression of four miRNAs was normalized to the expression of the internal control gene (U6).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4561872&req=5

fig5: Validation of miRNA microarray data by qRT-PCR. (a) miR-20a; (b) miR-145; (c) miRNA-98; (d) miR-30. The relative expression of four miRNAs was normalized to the expression of the internal control gene (U6).
Mentions: qRT-PCR is a quantitative and specific method that can be used to distinguish a single nucleotide difference between miRNAs. Thus, involution was obtained by miChip analysis for four selected miRNAs that showed either high (miR-145) or low (miR-30) signal intensities, or high (miR-20a) or low (miRNA-98) differential expression values among the three groups. The results of qRT-PCR analysis were often more reliable than those of the microarray analysis. qRT-PCR showed that miR-145 and miR-20a expression was downregulated in the model group compared with their expression in the PQR group, which was consistent with the microarray results. Thus, the miRNA expression profiles obtained by qRT-PCR fully support the results of miChip analysis (Figure 5).

Bottom Line: Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups.These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process.The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

View Article: PubMed Central - PubMed

Affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The present study aimed to investigate the relationship between miRNAs and in spontaneously hypertensive rats (SHR) vascular remodeling and analyze the impact of the Pinggan Qianyang recipe (PQR) on miRNAs. Mammalian miRNA microarrays containing 509 miRNA genes were employed to analyze the differentially expressed miRNAs in the three groups. MiRNAs were considered to be up- or downregulated when the fluorescent intensity ratio between the two groups was over 4-fold. Validation of those miRNAs changed in SHR after PQR treatment was used by real-time quantitative RT-PCR (qRT-PCR). Compared with the normal group, a total of 32 miRNAs were differentially expressed by more than twofold; among these, 18 were upregulated and 14 were downregulated in the model group. Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups. These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process. The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

No MeSH data available.