Limits...
Effects of the Pinggan Qianyang Recipe on MicroRNA Gene Expression in the Aortic Tissue of Spontaneously Hypertensive Rats.

Zhong G, Fang X, Wang D, Chen Q, Tang T - Evid Based Complement Alternat Med (2015)

Bottom Line: Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups.These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process.The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

View Article: PubMed Central - PubMed

Affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The present study aimed to investigate the relationship between miRNAs and in spontaneously hypertensive rats (SHR) vascular remodeling and analyze the impact of the Pinggan Qianyang recipe (PQR) on miRNAs. Mammalian miRNA microarrays containing 509 miRNA genes were employed to analyze the differentially expressed miRNAs in the three groups. MiRNAs were considered to be up- or downregulated when the fluorescent intensity ratio between the two groups was over 4-fold. Validation of those miRNAs changed in SHR after PQR treatment was used by real-time quantitative RT-PCR (qRT-PCR). Compared with the normal group, a total of 32 miRNAs were differentially expressed by more than twofold; among these, 18 were upregulated and 14 were downregulated in the model group. Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups. These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process. The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

No MeSH data available.


Detection of miRNA by microarray analysis. Total RNA extracted from three groups of rat aortic tissue were covalently labeled with Cy3 (green) and Cy5 (red) and hybridized to the array. The microarray slides contained two replicate subarrays. (a) Normal group and model group; (b) model group and PQR group.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4561872&req=5

fig4: Detection of miRNA by microarray analysis. Total RNA extracted from three groups of rat aortic tissue were covalently labeled with Cy3 (green) and Cy5 (red) and hybridized to the array. The microarray slides contained two replicate subarrays. (a) Normal group and model group; (b) model group and PQR group.

Mentions: To determine which miRNAs are potentially involved in the underlying mechanism of PQR treatment for essential hypertension, we tested miRNA levels in all rats by microarray analysis. We found that miRNA expression was remarkably aberrant in the model group compared with that of the normal group. In the model group, 32 of the 509 rat aortic miRNAs analyzed were differentially expressed (P < 0.01), with 18 miRNAs upregulated and 14 miRNAs downregulated. After 4 weeks of PQR treatment, we found that 17 of the 32 aortic miRNAs were differentially expressed; seven were upregulated and 10 were downregulated. Significant time course changes of miRNA expression were observed in the aortic tissue; more than 46.8% miRNAs were dysregulated (down- or upregulated) after PQR treatment (Figure 2(a)). All differential expression levels of miRNAs at three time points are listed in Figure 4 and Table 2. These data indicate that the development of essential hypertension involves a wave of expression of sequential classes of miRNAs. The temporal regulation of these miRNAs indicates that they might play an important role in PQR treatment of essential hypertension.


Effects of the Pinggan Qianyang Recipe on MicroRNA Gene Expression in the Aortic Tissue of Spontaneously Hypertensive Rats.

Zhong G, Fang X, Wang D, Chen Q, Tang T - Evid Based Complement Alternat Med (2015)

Detection of miRNA by microarray analysis. Total RNA extracted from three groups of rat aortic tissue were covalently labeled with Cy3 (green) and Cy5 (red) and hybridized to the array. The microarray slides contained two replicate subarrays. (a) Normal group and model group; (b) model group and PQR group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4561872&req=5

fig4: Detection of miRNA by microarray analysis. Total RNA extracted from three groups of rat aortic tissue were covalently labeled with Cy3 (green) and Cy5 (red) and hybridized to the array. The microarray slides contained two replicate subarrays. (a) Normal group and model group; (b) model group and PQR group.
Mentions: To determine which miRNAs are potentially involved in the underlying mechanism of PQR treatment for essential hypertension, we tested miRNA levels in all rats by microarray analysis. We found that miRNA expression was remarkably aberrant in the model group compared with that of the normal group. In the model group, 32 of the 509 rat aortic miRNAs analyzed were differentially expressed (P < 0.01), with 18 miRNAs upregulated and 14 miRNAs downregulated. After 4 weeks of PQR treatment, we found that 17 of the 32 aortic miRNAs were differentially expressed; seven were upregulated and 10 were downregulated. Significant time course changes of miRNA expression were observed in the aortic tissue; more than 46.8% miRNAs were dysregulated (down- or upregulated) after PQR treatment (Figure 2(a)). All differential expression levels of miRNAs at three time points are listed in Figure 4 and Table 2. These data indicate that the development of essential hypertension involves a wave of expression of sequential classes of miRNAs. The temporal regulation of these miRNAs indicates that they might play an important role in PQR treatment of essential hypertension.

Bottom Line: Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups.These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process.The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

View Article: PubMed Central - PubMed

Affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The present study aimed to investigate the relationship between miRNAs and in spontaneously hypertensive rats (SHR) vascular remodeling and analyze the impact of the Pinggan Qianyang recipe (PQR) on miRNAs. Mammalian miRNA microarrays containing 509 miRNA genes were employed to analyze the differentially expressed miRNAs in the three groups. MiRNAs were considered to be up- or downregulated when the fluorescent intensity ratio between the two groups was over 4-fold. Validation of those miRNAs changed in SHR after PQR treatment was used by real-time quantitative RT-PCR (qRT-PCR). Compared with the normal group, a total of 32 miRNAs were differentially expressed by more than twofold; among these, 18 were upregulated and 14 were downregulated in the model group. Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups. These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process. The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

No MeSH data available.