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Effects of the Pinggan Qianyang Recipe on MicroRNA Gene Expression in the Aortic Tissue of Spontaneously Hypertensive Rats.

Zhong G, Fang X, Wang D, Chen Q, Tang T - Evid Based Complement Alternat Med (2015)

Bottom Line: Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups.These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process.The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

View Article: PubMed Central - PubMed

Affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The present study aimed to investigate the relationship between miRNAs and in spontaneously hypertensive rats (SHR) vascular remodeling and analyze the impact of the Pinggan Qianyang recipe (PQR) on miRNAs. Mammalian miRNA microarrays containing 509 miRNA genes were employed to analyze the differentially expressed miRNAs in the three groups. MiRNAs were considered to be up- or downregulated when the fluorescent intensity ratio between the two groups was over 4-fold. Validation of those miRNAs changed in SHR after PQR treatment was used by real-time quantitative RT-PCR (qRT-PCR). Compared with the normal group, a total of 32 miRNAs were differentially expressed by more than twofold; among these, 18 were upregulated and 14 were downregulated in the model group. Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups. These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process. The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

No MeSH data available.


Related in: MedlinePlus

SBP changes in WKY rats or SHR receiving an i.a. of PQR or distilled water at various times. Data are shown as the mean ± SD for twenty rats of each group. P values for statistical significance were as ★P < 0.01, compared with the model group; ▲P < 0.05, and ◆P < 0.01, compared with the PQR group, respectively.
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fig1: SBP changes in WKY rats or SHR receiving an i.a. of PQR or distilled water at various times. Data are shown as the mean ± SD for twenty rats of each group. P values for statistical significance were as ★P < 0.01, compared with the model group; ▲P < 0.05, and ◆P < 0.01, compared with the PQR group, respectively.

Mentions: At the beginning of treatment, SBP was 126 ± 11 mmHg in the normal group and 208 ± 14 mmHg in the model and PQR groups (P < 0.01). However, a decrease in SBP was observed in the PQR group after 2 weeks of treatment (P < 0.05). After 4 weeks of treatment, the SBP of the PQR group was approximately 45 mmHg lower than at the beginning of treatment (Figure 1).


Effects of the Pinggan Qianyang Recipe on MicroRNA Gene Expression in the Aortic Tissue of Spontaneously Hypertensive Rats.

Zhong G, Fang X, Wang D, Chen Q, Tang T - Evid Based Complement Alternat Med (2015)

SBP changes in WKY rats or SHR receiving an i.a. of PQR or distilled water at various times. Data are shown as the mean ± SD for twenty rats of each group. P values for statistical significance were as ★P < 0.01, compared with the model group; ▲P < 0.05, and ◆P < 0.01, compared with the PQR group, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4561872&req=5

fig1: SBP changes in WKY rats or SHR receiving an i.a. of PQR or distilled water at various times. Data are shown as the mean ± SD for twenty rats of each group. P values for statistical significance were as ★P < 0.01, compared with the model group; ▲P < 0.05, and ◆P < 0.01, compared with the PQR group, respectively.
Mentions: At the beginning of treatment, SBP was 126 ± 11 mmHg in the normal group and 208 ± 14 mmHg in the model and PQR groups (P < 0.01). However, a decrease in SBP was observed in the PQR group after 2 weeks of treatment (P < 0.05). After 4 weeks of treatment, the SBP of the PQR group was approximately 45 mmHg lower than at the beginning of treatment (Figure 1).

Bottom Line: Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups.These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process.The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

View Article: PubMed Central - PubMed

Affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

ABSTRACT
The present study aimed to investigate the relationship between miRNAs and in spontaneously hypertensive rats (SHR) vascular remodeling and analyze the impact of the Pinggan Qianyang recipe (PQR) on miRNAs. Mammalian miRNA microarrays containing 509 miRNA genes were employed to analyze the differentially expressed miRNAs in the three groups. MiRNAs were considered to be up- or downregulated when the fluorescent intensity ratio between the two groups was over 4-fold. Validation of those miRNAs changed in SHR after PQR treatment was used by real-time quantitative RT-PCR (qRT-PCR). Compared with the normal group, a total of 32 miRNAs were differentially expressed by more than twofold; among these, 18 were upregulated and 14 were downregulated in the model group. Compared with the normal group, there were a number of 17 miRNAs which were significantly expressed by more than twofold in the different expressions of 32 miRNAs; among these, 10 were downregulated and 7 were upregulated in the PQR group. qRT-PCR verified that miR-20a, miR-145, miR-30, and miR-98 were significantly expressed in the three groups. These data show that PQR could exert its antihypertensive effect through deterioration of the vascular remodeling process. The mechanism might be associated with regulating differentially expressed miRNAs in aorta tissue.

No MeSH data available.


Related in: MedlinePlus