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Geriatric Respondents and Non-Respondents to Probiotic Intervention Can be Differentiated by Inherent Gut Microbiome Composition.

Senan S, Prajapati JB, Joshi CG, Sreeja V, Gohel MK, Trivedi S, Patel RM, Pandya H, Singh US, Phatak A, Patel HA - Front Microbiol (2015)

Bottom Line: Among respondents and non-respondents, the chief genera of phylum Firmicutes that showed significant differences are Lactobacillus, Clostridium, Eubacterium, and Blautia (q < 0.002), while in the genera of phylum Proteobacteria included Shigella, Escherichia, Burkholderia and Camphylobacter (q < 0.002).We have identified potential microbial biomarkers and taxonomic patterns that correlate with a positive response to probiotic intervention in geriatric volunteers.Future work with larger cohorts of geriatrics with diverse dietary influences could reveal the potential of the signature patterns of microbiota for personalized nutrition.

View Article: PubMed Central - PubMed

Affiliation: Department of Dairy Science, South Dakota State University , Brookings, SD , USA.

ABSTRACT

Scope: Probiotic interventions are known to have been shown to influence the composition of the intestinal microbiota in geriatrics. The growing concern is the apparent variation in response to identical strain dosage among human volunteers. One factor that governs this variation is the host gut microbiome. In this study, we attempted to define a core gut metagenome, which could act as a predisposition signature marker of inherent bacterial community that can help predict the success of a probiotic intervention.

Methods and results: To characterize the geriatric gut microbiome, we designed primers targeting the 16S rRNA hypervariable region V2-V3 followed by semiconductor sequencing using Ion Torrent PGM. Among respondents and non-respondents, the chief genera of phylum Firmicutes that showed significant differences are Lactobacillus, Clostridium, Eubacterium, and Blautia (q < 0.002), while in the genera of phylum Proteobacteria included Shigella, Escherichia, Burkholderia and Camphylobacter (q < 0.002).

Conclusion: We have identified potential microbial biomarkers and taxonomic patterns that correlate with a positive response to probiotic intervention in geriatric volunteers. Future work with larger cohorts of geriatrics with diverse dietary influences could reveal the potential of the signature patterns of microbiota for personalized nutrition.

No MeSH data available.


Related in: MedlinePlus

Inter-individual variation in the geriatric gut microbiome pre- and post-probiotic feeding.
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Figure 1: Inter-individual variation in the geriatric gut microbiome pre- and post-probiotic feeding.

Mentions: The relative abundance of major genera in the elderly gut metagenome and high-level of inter-individual variation is shown in Figure 1. We presume that the inter-individual differences are indicative of a highly personal fecal microbiota profile, which determines the response of the host to probiotics. Host factors probably play a major effect in shaping the intestinal microbial ecosystem during an intervention. In the present study, we attempted to understand the core microbiome of respondents and non-respondents to probiotics.


Geriatric Respondents and Non-Respondents to Probiotic Intervention Can be Differentiated by Inherent Gut Microbiome Composition.

Senan S, Prajapati JB, Joshi CG, Sreeja V, Gohel MK, Trivedi S, Patel RM, Pandya H, Singh US, Phatak A, Patel HA - Front Microbiol (2015)

Inter-individual variation in the geriatric gut microbiome pre- and post-probiotic feeding.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561823&req=5

Figure 1: Inter-individual variation in the geriatric gut microbiome pre- and post-probiotic feeding.
Mentions: The relative abundance of major genera in the elderly gut metagenome and high-level of inter-individual variation is shown in Figure 1. We presume that the inter-individual differences are indicative of a highly personal fecal microbiota profile, which determines the response of the host to probiotics. Host factors probably play a major effect in shaping the intestinal microbial ecosystem during an intervention. In the present study, we attempted to understand the core microbiome of respondents and non-respondents to probiotics.

Bottom Line: Among respondents and non-respondents, the chief genera of phylum Firmicutes that showed significant differences are Lactobacillus, Clostridium, Eubacterium, and Blautia (q < 0.002), while in the genera of phylum Proteobacteria included Shigella, Escherichia, Burkholderia and Camphylobacter (q < 0.002).We have identified potential microbial biomarkers and taxonomic patterns that correlate with a positive response to probiotic intervention in geriatric volunteers.Future work with larger cohorts of geriatrics with diverse dietary influences could reveal the potential of the signature patterns of microbiota for personalized nutrition.

View Article: PubMed Central - PubMed

Affiliation: Department of Dairy Science, South Dakota State University , Brookings, SD , USA.

ABSTRACT

Scope: Probiotic interventions are known to have been shown to influence the composition of the intestinal microbiota in geriatrics. The growing concern is the apparent variation in response to identical strain dosage among human volunteers. One factor that governs this variation is the host gut microbiome. In this study, we attempted to define a core gut metagenome, which could act as a predisposition signature marker of inherent bacterial community that can help predict the success of a probiotic intervention.

Methods and results: To characterize the geriatric gut microbiome, we designed primers targeting the 16S rRNA hypervariable region V2-V3 followed by semiconductor sequencing using Ion Torrent PGM. Among respondents and non-respondents, the chief genera of phylum Firmicutes that showed significant differences are Lactobacillus, Clostridium, Eubacterium, and Blautia (q < 0.002), while in the genera of phylum Proteobacteria included Shigella, Escherichia, Burkholderia and Camphylobacter (q < 0.002).

Conclusion: We have identified potential microbial biomarkers and taxonomic patterns that correlate with a positive response to probiotic intervention in geriatric volunteers. Future work with larger cohorts of geriatrics with diverse dietary influences could reveal the potential of the signature patterns of microbiota for personalized nutrition.

No MeSH data available.


Related in: MedlinePlus