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Natural variation in cross-talk between glucosinolates and onset of flowering in Arabidopsis.

Jensen LM, Jepsen HS, Halkier BA, Kliebenstein DJ, Burow M - Front Plant Sci (2015)

Bottom Line: We have introduced the two highly similar enzymes into two different AOP () accessions, Col-0 and Cph-0, and found that the genes differ in their ability to affect glucosinolate levels and flowering time across the accessions.This indicated that the different glucosinolates produced by AOP2 and AOP3 serve specific regulatory roles in controlling these phenotypes.This variation likely reflects an adaptation to survival in different environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant and Environmental Sciences, Faculty of Science, DNRF Center DynaMo, University of Copenhagen Frederiksberg, Denmark ; Department of Plant and Environmental Sciences, Faculty of Science, Copenhagen Plant Science Centre, University of Copenhagen Frederiksberg, Denmark.

ABSTRACT
Naturally variable regulatory networks control different biological processes including reproduction and defense. This variation within regulatory networks enables plants to optimize defense and reproduction in different environments. In this study we investigate the ability of two enzyme-encoding genes in the glucosinolate pathway, AOP2 and AOP3, to affect glucosinolate accumulation and flowering time. We have introduced the two highly similar enzymes into two different AOP () accessions, Col-0 and Cph-0, and found that the genes differ in their ability to affect glucosinolate levels and flowering time across the accessions. This indicated that the different glucosinolates produced by AOP2 and AOP3 serve specific regulatory roles in controlling these phenotypes. While the changes in glucosinolate levels were similar in both accessions, the effect on flowering time was dependent on the genetic background pointing to natural variation in cross-talk between defense chemistry and onset of flowering. This variation likely reflects an adaptation to survival in different environments.

No MeSH data available.


Related in: MedlinePlus

Effects of AOP2 and AOP3 on glucosinolate accumulation in Col-0. Glucosinolate concentrations in leaves of Col-0 WT (black), n = 28, Col-0 AOP2 (light gray), n = 18 (2 independent insertion lines), and Col-0 AOP3 (dark gray), n = 25 (1 line), as (A) Total SC and LC, (B) C3 glucosinolates, and (C) C4 substrate and products. Means (+ standard deviations) are shown for two experimental repeats of the same lines. Nested ANOVA across independent lines and post testing was used for statistical analysis. Letters indicate significant differences (P < 0.05) between genotypes.
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Figure 3: Effects of AOP2 and AOP3 on glucosinolate accumulation in Col-0. Glucosinolate concentrations in leaves of Col-0 WT (black), n = 28, Col-0 AOP2 (light gray), n = 18 (2 independent insertion lines), and Col-0 AOP3 (dark gray), n = 25 (1 line), as (A) Total SC and LC, (B) C3 glucosinolates, and (C) C4 substrate and products. Means (+ standard deviations) are shown for two experimental repeats of the same lines. Nested ANOVA across independent lines and post testing was used for statistical analysis. Letters indicate significant differences (P < 0.05) between genotypes.

Mentions: In agreement with previous work using the Brassica oleracea or Arabidopsis accession Pi AOP2 (Li and Quiros, 2003; Wentzell et al., 2007; Neal et al., 2010), constitutive expression of AOP2 in the Col-0 background lead to the formation of 2-propenyl and 3-butenyl glucosinolate from 3msp to 4msb, respectively (Figures 2, 3B,C, for all individual glucosinolates see Figure S1). The presence of a functional GS-OH within Col-0 further modified the 3-butenyl side chain to 2R- and 2S-2-hydroxy-3-butenyl (Figure 3C), (Hansen et al., 2008). AOP3 expression in Col-0 led to the conversion of 3msp to 3ohp and interestingly, we also detected small amounts of 4-hydroxybutyl glucosinolate (4ohb), (Figures 3B,C). Thus, AOP3 appears to be able to convert 4msb to 4ohb in planta, an activity that could not be detected in vitro (Kliebenstein et al., 2001c). However, based on the ratios of substrates and products in Col-0, AOP3 seems to have a preference for 3msp.


Natural variation in cross-talk between glucosinolates and onset of flowering in Arabidopsis.

Jensen LM, Jepsen HS, Halkier BA, Kliebenstein DJ, Burow M - Front Plant Sci (2015)

Effects of AOP2 and AOP3 on glucosinolate accumulation in Col-0. Glucosinolate concentrations in leaves of Col-0 WT (black), n = 28, Col-0 AOP2 (light gray), n = 18 (2 independent insertion lines), and Col-0 AOP3 (dark gray), n = 25 (1 line), as (A) Total SC and LC, (B) C3 glucosinolates, and (C) C4 substrate and products. Means (+ standard deviations) are shown for two experimental repeats of the same lines. Nested ANOVA across independent lines and post testing was used for statistical analysis. Letters indicate significant differences (P < 0.05) between genotypes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561820&req=5

Figure 3: Effects of AOP2 and AOP3 on glucosinolate accumulation in Col-0. Glucosinolate concentrations in leaves of Col-0 WT (black), n = 28, Col-0 AOP2 (light gray), n = 18 (2 independent insertion lines), and Col-0 AOP3 (dark gray), n = 25 (1 line), as (A) Total SC and LC, (B) C3 glucosinolates, and (C) C4 substrate and products. Means (+ standard deviations) are shown for two experimental repeats of the same lines. Nested ANOVA across independent lines and post testing was used for statistical analysis. Letters indicate significant differences (P < 0.05) between genotypes.
Mentions: In agreement with previous work using the Brassica oleracea or Arabidopsis accession Pi AOP2 (Li and Quiros, 2003; Wentzell et al., 2007; Neal et al., 2010), constitutive expression of AOP2 in the Col-0 background lead to the formation of 2-propenyl and 3-butenyl glucosinolate from 3msp to 4msb, respectively (Figures 2, 3B,C, for all individual glucosinolates see Figure S1). The presence of a functional GS-OH within Col-0 further modified the 3-butenyl side chain to 2R- and 2S-2-hydroxy-3-butenyl (Figure 3C), (Hansen et al., 2008). AOP3 expression in Col-0 led to the conversion of 3msp to 3ohp and interestingly, we also detected small amounts of 4-hydroxybutyl glucosinolate (4ohb), (Figures 3B,C). Thus, AOP3 appears to be able to convert 4msb to 4ohb in planta, an activity that could not be detected in vitro (Kliebenstein et al., 2001c). However, based on the ratios of substrates and products in Col-0, AOP3 seems to have a preference for 3msp.

Bottom Line: We have introduced the two highly similar enzymes into two different AOP () accessions, Col-0 and Cph-0, and found that the genes differ in their ability to affect glucosinolate levels and flowering time across the accessions.This indicated that the different glucosinolates produced by AOP2 and AOP3 serve specific regulatory roles in controlling these phenotypes.This variation likely reflects an adaptation to survival in different environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant and Environmental Sciences, Faculty of Science, DNRF Center DynaMo, University of Copenhagen Frederiksberg, Denmark ; Department of Plant and Environmental Sciences, Faculty of Science, Copenhagen Plant Science Centre, University of Copenhagen Frederiksberg, Denmark.

ABSTRACT
Naturally variable regulatory networks control different biological processes including reproduction and defense. This variation within regulatory networks enables plants to optimize defense and reproduction in different environments. In this study we investigate the ability of two enzyme-encoding genes in the glucosinolate pathway, AOP2 and AOP3, to affect glucosinolate accumulation and flowering time. We have introduced the two highly similar enzymes into two different AOP () accessions, Col-0 and Cph-0, and found that the genes differ in their ability to affect glucosinolate levels and flowering time across the accessions. This indicated that the different glucosinolates produced by AOP2 and AOP3 serve specific regulatory roles in controlling these phenotypes. While the changes in glucosinolate levels were similar in both accessions, the effect on flowering time was dependent on the genetic background pointing to natural variation in cross-talk between defense chemistry and onset of flowering. This variation likely reflects an adaptation to survival in different environments.

No MeSH data available.


Related in: MedlinePlus