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Cryotolerance of apple tree bud is independent of endodormancy.

Bilavcik A, Zamecnik J, Faltus M - Front Plant Sci (2015)

Bottom Line: The cryosurvival of vegetative apple buds of both cultivars correlated with their cold hardening without direct regard to their particular phase of dormancy.Both cultivars had the highest cryosurvival in December and January.The presented data were compared with our previous results from a dormancy study of in vitro apple culture.

View Article: PubMed Central - PubMed

Affiliation: Plant Physiology and Cryobiology Laboratory, Crop Research Institute Prague, Czech Republic.

ABSTRACT
Increasing interest in cryopreservation of dormant buds reveals the need for better understanding of the role of dormancy in cryotolerance. Dormancy stage and low-temperature survival of vegetative apple buds (Malus domestica Borkh.), cultivars 'Sampion' and 'Spartan', collected from orchard were evaluated during three seasons contrasting in temperature and precipitation throughout the arrested plant growth period. During each season, the cultivars differed either in the onset of the endodormancy or in the length of the endodormant period. A simple relation between endodormancy of the buds and their water content was not detected. The cryosurvival of vegetative apple buds of both cultivars correlated with their cold hardening without direct regard to their particular phase of dormancy. The period of the highest bud cryotolerance after low-temperature exposure overlapped with the endodormant period in some evaluated seasons. Both cultivars had the highest cryosurvival in December and January. The presented data were compared with our previous results from a dormancy study of in vitro apple culture. Endodormancy coincided with the period of successful cryosurvival of apple buds after liquid nitrogen exposure, but as such, it was not decisive for their survival and did not limit their successful cryopreservation.

No MeSH data available.


Related in: MedlinePlus

Survival and water content of non-dehydrated (A) and dehydrated (B) vegetative buds of apple cultivars ‘Sampion’ and ‘Spartan’ (B) in the seasons of 1999/2000. ND was cryopreserved immediately after sampling from the orchard. D variant was cryopreserved after frost dehydration of at least 1 week at –4°C. Survival was evaluated after the first step of cryopreservation protocol at –30°C (–30), and after the second step, reaching the LN temperature. Survival was tested by examination of oxidative browning of tissues on the longitudinal cut of the bud. Water content was measured immediately before cryopreservation procedure. Bars indicate SD (p < 0.05).
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Figure 5: Survival and water content of non-dehydrated (A) and dehydrated (B) vegetative buds of apple cultivars ‘Sampion’ and ‘Spartan’ (B) in the seasons of 1999/2000. ND was cryopreserved immediately after sampling from the orchard. D variant was cryopreserved after frost dehydration of at least 1 week at –4°C. Survival was evaluated after the first step of cryopreservation protocol at –30°C (–30), and after the second step, reaching the LN temperature. Survival was tested by examination of oxidative browning of tissues on the longitudinal cut of the bud. Water content was measured immediately before cryopreservation procedure. Bars indicate SD (p < 0.05).

Mentions: The survival of ‘Sampion’ and ‘Spartan’ D-30 variant ranged from 4 to 93% and from 0 to 76%, respectively. The survival of ‘Sampion’ and ‘Spartan’ DLN variant ranged from 0 to 74% and from 0 to 69%, respectively. Water content of ‘Sampion’ and ‘Spartan’ dormant buds after dehydration ranged from 0.37 to 0.50 gH2O g-1DM and from 0.40 to 0.50 gH2O g-1DM, respectively. The dehydration time of the D variant ranged from 8 to 36 days. The course of survival of ‘Sampion’ and ‘Spartan’ dormant buds during their cryopreservation sampled in the winter period of 1999/2000 is shown in Figure 5.


Cryotolerance of apple tree bud is independent of endodormancy.

Bilavcik A, Zamecnik J, Faltus M - Front Plant Sci (2015)

Survival and water content of non-dehydrated (A) and dehydrated (B) vegetative buds of apple cultivars ‘Sampion’ and ‘Spartan’ (B) in the seasons of 1999/2000. ND was cryopreserved immediately after sampling from the orchard. D variant was cryopreserved after frost dehydration of at least 1 week at –4°C. Survival was evaluated after the first step of cryopreservation protocol at –30°C (–30), and after the second step, reaching the LN temperature. Survival was tested by examination of oxidative browning of tissues on the longitudinal cut of the bud. Water content was measured immediately before cryopreservation procedure. Bars indicate SD (p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561819&req=5

Figure 5: Survival and water content of non-dehydrated (A) and dehydrated (B) vegetative buds of apple cultivars ‘Sampion’ and ‘Spartan’ (B) in the seasons of 1999/2000. ND was cryopreserved immediately after sampling from the orchard. D variant was cryopreserved after frost dehydration of at least 1 week at –4°C. Survival was evaluated after the first step of cryopreservation protocol at –30°C (–30), and after the second step, reaching the LN temperature. Survival was tested by examination of oxidative browning of tissues on the longitudinal cut of the bud. Water content was measured immediately before cryopreservation procedure. Bars indicate SD (p < 0.05).
Mentions: The survival of ‘Sampion’ and ‘Spartan’ D-30 variant ranged from 4 to 93% and from 0 to 76%, respectively. The survival of ‘Sampion’ and ‘Spartan’ DLN variant ranged from 0 to 74% and from 0 to 69%, respectively. Water content of ‘Sampion’ and ‘Spartan’ dormant buds after dehydration ranged from 0.37 to 0.50 gH2O g-1DM and from 0.40 to 0.50 gH2O g-1DM, respectively. The dehydration time of the D variant ranged from 8 to 36 days. The course of survival of ‘Sampion’ and ‘Spartan’ dormant buds during their cryopreservation sampled in the winter period of 1999/2000 is shown in Figure 5.

Bottom Line: The cryosurvival of vegetative apple buds of both cultivars correlated with their cold hardening without direct regard to their particular phase of dormancy.Both cultivars had the highest cryosurvival in December and January.The presented data were compared with our previous results from a dormancy study of in vitro apple culture.

View Article: PubMed Central - PubMed

Affiliation: Plant Physiology and Cryobiology Laboratory, Crop Research Institute Prague, Czech Republic.

ABSTRACT
Increasing interest in cryopreservation of dormant buds reveals the need for better understanding of the role of dormancy in cryotolerance. Dormancy stage and low-temperature survival of vegetative apple buds (Malus domestica Borkh.), cultivars 'Sampion' and 'Spartan', collected from orchard were evaluated during three seasons contrasting in temperature and precipitation throughout the arrested plant growth period. During each season, the cultivars differed either in the onset of the endodormancy or in the length of the endodormant period. A simple relation between endodormancy of the buds and their water content was not detected. The cryosurvival of vegetative apple buds of both cultivars correlated with their cold hardening without direct regard to their particular phase of dormancy. The period of the highest bud cryotolerance after low-temperature exposure overlapped with the endodormant period in some evaluated seasons. Both cultivars had the highest cryosurvival in December and January. The presented data were compared with our previous results from a dormancy study of in vitro apple culture. Endodormancy coincided with the period of successful cryosurvival of apple buds after liquid nitrogen exposure, but as such, it was not decisive for their survival and did not limit their successful cryopreservation.

No MeSH data available.


Related in: MedlinePlus