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Neurochemical measurements in the zebrafish brain.

Jones LJ, McCutcheon JE, Young AM, Norton WH - Front Behav Neurosci (2015)

Bottom Line: In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon.We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain.This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Psychology and Behaviour, University of Leicester Leicester, UK.

ABSTRACT
The zebrafish is an ideal model organism for behavioral genetics and neuroscience. The high conservation of genes and neurotransmitter pathways between zebrafish and other vertebrates permits the translation of research between species. Zebrafish behavior can be studied at both larval and adult stages and recent research has begun to establish zebrafish models for human disease. Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that permits the detection of neurotransmitter release and reuptake. In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon. We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain. This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.

No MeSH data available.


Related in: MedlinePlus

Statistical analysis of stimulated release. (A) Template voltammogram resulting from exposing an electrode in a flow cell to 2 μM dopamine and 80 μM histamine in the presence of an acidic pH shift (−0.25 pH units, pH 7.4 → pH 7.15). (B) Template voltammogram resulting from exposing an electrode in a flow cell to 2 μM dopamine and 20 μM histamine. (C–F) Cyclic voltammograms obtained by either electrical- (C,E) or high K+ aCSF stimulation (D,F) of the telencephalon. The r2-value for (A,C) is 0.846; the r2-value for (A,E) is 0.833; the r2-value for (B,D) is 0.857 and the r2-value for (B,F) is 0.416.
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Figure 7: Statistical analysis of stimulated release. (A) Template voltammogram resulting from exposing an electrode in a flow cell to 2 μM dopamine and 80 μM histamine in the presence of an acidic pH shift (−0.25 pH units, pH 7.4 → pH 7.15). (B) Template voltammogram resulting from exposing an electrode in a flow cell to 2 μM dopamine and 20 μM histamine. (C–F) Cyclic voltammograms obtained by either electrical- (C,E) or high K+ aCSF stimulation (D,F) of the telencephalon. The r2-value for (A,C) is 0.846; the r2-value for (A,E) is 0.833; the r2-value for (B,D) is 0.857 and the r2-value for (B,F) is 0.416.

Mentions: In order to compare the similarity of experimental recordings in tissue to template flow cell data we used the CV match function in TarHeel. We calculated r2-values between experimental cyclic voltammograms from the telencephalon obtained using optimal stimulation parameters and multiple template cyclic voltammograms (Robinson and Wightman, 2007). Comparison of voltammograms produced by electrical stimulation (four experiments from two sagittal sections taken from two fish) to a mixture of 2 μM dopamine, 80 μM histamine and an acidic pH shift of −0.25 units produced r2-values that exceeded the accepted threshold of 0.75 in 3 out of 4 stimulations analyzed (for example, Figures 7A,C, r2 = 0.846; Figures 7A,E, r2 = 0.833; for further values see Table 1). In one case, a template of 4 μM dopamine, 160 μM histamine and an acidic pH shift of −0.25 units produced the highest r2-value of 0.878 (see Table 1). High K+ aCSF stimulation of the telencephalon produced a voltammogram at ~10 s following stimulation, which we also compared to multiple template cyclic voltammograms (two experiments from two fish) that were significantly similar to a combination of 2 μM dopamine and 20 μM histamine (Figures 7B,D, r2 = 0.857; for further values see Table 1). However, it was not possible to produce a template voltammogram in the flow cell that gave a significant match to a voltammogram extracted at ~30 s following high K+ aCSF stimulation. In summary, the analytes released by electrical stimulation of the zebrafish forebrain are significantly similar to a combination of dopamine, histamine and an acidic pH change of −0.25 units, whereas the analytes released ~10 s after high K+ stimulation are indicated to be more similar to a combination of dopamine and histamine.


Neurochemical measurements in the zebrafish brain.

Jones LJ, McCutcheon JE, Young AM, Norton WH - Front Behav Neurosci (2015)

Statistical analysis of stimulated release. (A) Template voltammogram resulting from exposing an electrode in a flow cell to 2 μM dopamine and 80 μM histamine in the presence of an acidic pH shift (−0.25 pH units, pH 7.4 → pH 7.15). (B) Template voltammogram resulting from exposing an electrode in a flow cell to 2 μM dopamine and 20 μM histamine. (C–F) Cyclic voltammograms obtained by either electrical- (C,E) or high K+ aCSF stimulation (D,F) of the telencephalon. The r2-value for (A,C) is 0.846; the r2-value for (A,E) is 0.833; the r2-value for (B,D) is 0.857 and the r2-value for (B,F) is 0.416.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561813&req=5

Figure 7: Statistical analysis of stimulated release. (A) Template voltammogram resulting from exposing an electrode in a flow cell to 2 μM dopamine and 80 μM histamine in the presence of an acidic pH shift (−0.25 pH units, pH 7.4 → pH 7.15). (B) Template voltammogram resulting from exposing an electrode in a flow cell to 2 μM dopamine and 20 μM histamine. (C–F) Cyclic voltammograms obtained by either electrical- (C,E) or high K+ aCSF stimulation (D,F) of the telencephalon. The r2-value for (A,C) is 0.846; the r2-value for (A,E) is 0.833; the r2-value for (B,D) is 0.857 and the r2-value for (B,F) is 0.416.
Mentions: In order to compare the similarity of experimental recordings in tissue to template flow cell data we used the CV match function in TarHeel. We calculated r2-values between experimental cyclic voltammograms from the telencephalon obtained using optimal stimulation parameters and multiple template cyclic voltammograms (Robinson and Wightman, 2007). Comparison of voltammograms produced by electrical stimulation (four experiments from two sagittal sections taken from two fish) to a mixture of 2 μM dopamine, 80 μM histamine and an acidic pH shift of −0.25 units produced r2-values that exceeded the accepted threshold of 0.75 in 3 out of 4 stimulations analyzed (for example, Figures 7A,C, r2 = 0.846; Figures 7A,E, r2 = 0.833; for further values see Table 1). In one case, a template of 4 μM dopamine, 160 μM histamine and an acidic pH shift of −0.25 units produced the highest r2-value of 0.878 (see Table 1). High K+ aCSF stimulation of the telencephalon produced a voltammogram at ~10 s following stimulation, which we also compared to multiple template cyclic voltammograms (two experiments from two fish) that were significantly similar to a combination of 2 μM dopamine and 20 μM histamine (Figures 7B,D, r2 = 0.857; for further values see Table 1). However, it was not possible to produce a template voltammogram in the flow cell that gave a significant match to a voltammogram extracted at ~30 s following high K+ aCSF stimulation. In summary, the analytes released by electrical stimulation of the zebrafish forebrain are significantly similar to a combination of dopamine, histamine and an acidic pH change of −0.25 units, whereas the analytes released ~10 s after high K+ stimulation are indicated to be more similar to a combination of dopamine and histamine.

Bottom Line: In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon.We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain.This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Psychology and Behaviour, University of Leicester Leicester, UK.

ABSTRACT
The zebrafish is an ideal model organism for behavioral genetics and neuroscience. The high conservation of genes and neurotransmitter pathways between zebrafish and other vertebrates permits the translation of research between species. Zebrafish behavior can be studied at both larval and adult stages and recent research has begun to establish zebrafish models for human disease. Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that permits the detection of neurotransmitter release and reuptake. In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon. We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain. This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.

No MeSH data available.


Related in: MedlinePlus