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Neurochemical measurements in the zebrafish brain.

Jones LJ, McCutcheon JE, Young AM, Norton WH - Front Behav Neurosci (2015)

Bottom Line: In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon.We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain.This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Psychology and Behaviour, University of Leicester Leicester, UK.

ABSTRACT
The zebrafish is an ideal model organism for behavioral genetics and neuroscience. The high conservation of genes and neurotransmitter pathways between zebrafish and other vertebrates permits the translation of research between species. Zebrafish behavior can be studied at both larval and adult stages and recent research has begun to establish zebrafish models for human disease. Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that permits the detection of neurotransmitter release and reuptake. In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon. We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain. This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.

No MeSH data available.


Fast scan cyclic voltammetry (FCSV) setup and position of the stimulating- and recording electrodes. (A) Diagram showing components of the FSCV setup and the position of the recording electrode in the adult zebrafish brain. The applied voltage waveform (top graph) and a representative cyclic voltammogram for dopamine (lower graph) are also shown, with the forward scan in black and the reverse scan in red. (B) Schematic representation showing a lateral and dorsal view of the adult zebrafish brain. The black asterisk marks the position of the recording electrode in the telencephalon. (C) Diagram showing characteristic non-Faradaic background signal that is subtracted to generate the background-subtracted voltammograms shown throughout the paper. Abbreviations: Cb, cerebellum; Hy, hypothalamus; OB, olfactory bulb; T, telencephalon; TeO, optic tectum.
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Figure 1: Fast scan cyclic voltammetry (FCSV) setup and position of the stimulating- and recording electrodes. (A) Diagram showing components of the FSCV setup and the position of the recording electrode in the adult zebrafish brain. The applied voltage waveform (top graph) and a representative cyclic voltammogram for dopamine (lower graph) are also shown, with the forward scan in black and the reverse scan in red. (B) Schematic representation showing a lateral and dorsal view of the adult zebrafish brain. The black asterisk marks the position of the recording electrode in the telencephalon. (C) Diagram showing characteristic non-Faradaic background signal that is subtracted to generate the background-subtracted voltammograms shown throughout the paper. Abbreviations: Cb, cerebellum; Hy, hypothalamus; OB, olfactory bulb; T, telencephalon; TeO, optic tectum.

Mentions: The setup for FSCV was custom built and consists of a tissue bath, stimulating electrode, recording and reference electrodes connected to a computer and amplifier (Figure 1A). Carbon fiber microelectrodes (tip size 7 × 120 μm) were used as recording electrodes and an Ag/AgCl electrode as a reference. The recording and reference electrodes were connected to a potentiostat and headstage circuit (ChemClamp, Dagan Instruments, USA) and a computer running TarHeel (Chapel Hill, University of North Carolina) voltammetry software. The waveform (Figure 1A) was applied at 10 Hz.


Neurochemical measurements in the zebrafish brain.

Jones LJ, McCutcheon JE, Young AM, Norton WH - Front Behav Neurosci (2015)

Fast scan cyclic voltammetry (FCSV) setup and position of the stimulating- and recording electrodes. (A) Diagram showing components of the FSCV setup and the position of the recording electrode in the adult zebrafish brain. The applied voltage waveform (top graph) and a representative cyclic voltammogram for dopamine (lower graph) are also shown, with the forward scan in black and the reverse scan in red. (B) Schematic representation showing a lateral and dorsal view of the adult zebrafish brain. The black asterisk marks the position of the recording electrode in the telencephalon. (C) Diagram showing characteristic non-Faradaic background signal that is subtracted to generate the background-subtracted voltammograms shown throughout the paper. Abbreviations: Cb, cerebellum; Hy, hypothalamus; OB, olfactory bulb; T, telencephalon; TeO, optic tectum.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561813&req=5

Figure 1: Fast scan cyclic voltammetry (FCSV) setup and position of the stimulating- and recording electrodes. (A) Diagram showing components of the FSCV setup and the position of the recording electrode in the adult zebrafish brain. The applied voltage waveform (top graph) and a representative cyclic voltammogram for dopamine (lower graph) are also shown, with the forward scan in black and the reverse scan in red. (B) Schematic representation showing a lateral and dorsal view of the adult zebrafish brain. The black asterisk marks the position of the recording electrode in the telencephalon. (C) Diagram showing characteristic non-Faradaic background signal that is subtracted to generate the background-subtracted voltammograms shown throughout the paper. Abbreviations: Cb, cerebellum; Hy, hypothalamus; OB, olfactory bulb; T, telencephalon; TeO, optic tectum.
Mentions: The setup for FSCV was custom built and consists of a tissue bath, stimulating electrode, recording and reference electrodes connected to a computer and amplifier (Figure 1A). Carbon fiber microelectrodes (tip size 7 × 120 μm) were used as recording electrodes and an Ag/AgCl electrode as a reference. The recording and reference electrodes were connected to a potentiostat and headstage circuit (ChemClamp, Dagan Instruments, USA) and a computer running TarHeel (Chapel Hill, University of North Carolina) voltammetry software. The waveform (Figure 1A) was applied at 10 Hz.

Bottom Line: In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon.We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain.This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Psychology and Behaviour, University of Leicester Leicester, UK.

ABSTRACT
The zebrafish is an ideal model organism for behavioral genetics and neuroscience. The high conservation of genes and neurotransmitter pathways between zebrafish and other vertebrates permits the translation of research between species. Zebrafish behavior can be studied at both larval and adult stages and recent research has begun to establish zebrafish models for human disease. Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that permits the detection of neurotransmitter release and reuptake. In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon. We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain. This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.

No MeSH data available.