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Effect of Glucagon-like Peptide-1 on the Differentiation of Adipose-derived Stem Cells into Osteoblasts and Adipocytes.

Lee HM, Joo BS, Lee CH, Kim HY, Ock JH, Lee YS - J Menopausal Med (2015)

Bottom Line: In contrast, GLP-1 significantly suppressed the expression of adipocyte-specific markers, peroxisome proliferator-activated receptor gamma (PPAR-γ), lipoprotein lipase (LPL) and adipocyte protein 2 (AP2).This decreased expression of adipocyte specific markers caused by GLP-1 was significantly reversed by the treatment of extracellular signal-regulated kinase (ERK) inhibitor, PD98059 (P < 0.05).This result demonstrates that GLP-1 stimulates osteoblast differentiation in ADSCs, whereas it inhibits adipocyte differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Natural Science College, Pusan National University, Busan, Korea.

ABSTRACT

Objectives: Glucagon-like peptide-1 (GLP-1) is an intestinally secreted hormone and it plays an important role in the regulation of glucose homeostasis. However, the possible role of GLP-1 in the differentiation of adipose-derived stem cells (ADSCs) remains unknown. Therefore this study investigated the effect of GLP-1 on the differentiation of ADSCs into osteoblasts and adipocytes.

Methods: ADSCs were isolated from human adipose tissues of the abdomens, cultured and characterized by flow cytometry and multi-lineage potential assay. ADSCs were induced in osteogenic and adipogenic media treated with two different doses (10 and 100 nM) of GLP-1, and then the effect of GLP-1 on differentiation of ADSCs into osteoblast and adipocyte was examined. The signaling pathway involved in these processes was also examined.

Results: Isolated human ADSCs expressed mesenchymal stem cell (MSC) specific markers as well as GLP-1 receptor (GLP-1R) proteins. They also showed multiple-lineage potential of MSC. GLP-1 was upregulated the activity and mRNA expression of osteoblast-specific marker, alkaline phosphatase and the mineralization of calcium. In contrast, GLP-1 significantly suppressed the expression of adipocyte-specific markers, peroxisome proliferator-activated receptor gamma (PPAR-γ), lipoprotein lipase (LPL) and adipocyte protein 2 (AP2). This decreased expression of adipocyte specific markers caused by GLP-1 was significantly reversed by the treatment of extracellular signal-regulated kinase (ERK) inhibitor, PD98059 (P < 0.05).

Conclusion: This result demonstrates that GLP-1 stimulates osteoblast differentiation in ADSCs, whereas it inhibits adipocyte differentiation. The ERK signaling pathway seems to be involved in these differentiation processes mediated by GLP-1.

No MeSH data available.


Glucagon-like peptide-1 (GLP-1) receptor expression in adipose-derived stem cells (ADSCs) was detected by immunofluorescence staining (green). Nuclei were labeled with DAPI (blue). Original magnification, × 200.
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Figure 2: Glucagon-like peptide-1 (GLP-1) receptor expression in adipose-derived stem cells (ADSCs) was detected by immunofluorescence staining (green). Nuclei were labeled with DAPI (blue). Original magnification, × 200.

Mentions: Prior to the examination of the effect of GLP-1 on adipogenic and osteogenic differentiation, whether GLP-1R is expressed in ADSCs was tested by immunofluorescence staining using anti-GLP-1R antibody. Fig. 2 showed GLP-1R positive stained cells.


Effect of Glucagon-like Peptide-1 on the Differentiation of Adipose-derived Stem Cells into Osteoblasts and Adipocytes.

Lee HM, Joo BS, Lee CH, Kim HY, Ock JH, Lee YS - J Menopausal Med (2015)

Glucagon-like peptide-1 (GLP-1) receptor expression in adipose-derived stem cells (ADSCs) was detected by immunofluorescence staining (green). Nuclei were labeled with DAPI (blue). Original magnification, × 200.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561747&req=5

Figure 2: Glucagon-like peptide-1 (GLP-1) receptor expression in adipose-derived stem cells (ADSCs) was detected by immunofluorescence staining (green). Nuclei were labeled with DAPI (blue). Original magnification, × 200.
Mentions: Prior to the examination of the effect of GLP-1 on adipogenic and osteogenic differentiation, whether GLP-1R is expressed in ADSCs was tested by immunofluorescence staining using anti-GLP-1R antibody. Fig. 2 showed GLP-1R positive stained cells.

Bottom Line: In contrast, GLP-1 significantly suppressed the expression of adipocyte-specific markers, peroxisome proliferator-activated receptor gamma (PPAR-γ), lipoprotein lipase (LPL) and adipocyte protein 2 (AP2).This decreased expression of adipocyte specific markers caused by GLP-1 was significantly reversed by the treatment of extracellular signal-regulated kinase (ERK) inhibitor, PD98059 (P < 0.05).This result demonstrates that GLP-1 stimulates osteoblast differentiation in ADSCs, whereas it inhibits adipocyte differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Natural Science College, Pusan National University, Busan, Korea.

ABSTRACT

Objectives: Glucagon-like peptide-1 (GLP-1) is an intestinally secreted hormone and it plays an important role in the regulation of glucose homeostasis. However, the possible role of GLP-1 in the differentiation of adipose-derived stem cells (ADSCs) remains unknown. Therefore this study investigated the effect of GLP-1 on the differentiation of ADSCs into osteoblasts and adipocytes.

Methods: ADSCs were isolated from human adipose tissues of the abdomens, cultured and characterized by flow cytometry and multi-lineage potential assay. ADSCs were induced in osteogenic and adipogenic media treated with two different doses (10 and 100 nM) of GLP-1, and then the effect of GLP-1 on differentiation of ADSCs into osteoblast and adipocyte was examined. The signaling pathway involved in these processes was also examined.

Results: Isolated human ADSCs expressed mesenchymal stem cell (MSC) specific markers as well as GLP-1 receptor (GLP-1R) proteins. They also showed multiple-lineage potential of MSC. GLP-1 was upregulated the activity and mRNA expression of osteoblast-specific marker, alkaline phosphatase and the mineralization of calcium. In contrast, GLP-1 significantly suppressed the expression of adipocyte-specific markers, peroxisome proliferator-activated receptor gamma (PPAR-γ), lipoprotein lipase (LPL) and adipocyte protein 2 (AP2). This decreased expression of adipocyte specific markers caused by GLP-1 was significantly reversed by the treatment of extracellular signal-regulated kinase (ERK) inhibitor, PD98059 (P < 0.05).

Conclusion: This result demonstrates that GLP-1 stimulates osteoblast differentiation in ADSCs, whereas it inhibits adipocyte differentiation. The ERK signaling pathway seems to be involved in these differentiation processes mediated by GLP-1.

No MeSH data available.