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Expression of Vitamin D Receptor in Seminal Vesicles of Cholesterol Formula Mice.

Kim TH, Lee HH, Kim JM, Choi SD, Lee A, Hwang SY, Kim M, Kim YS, Yeom S - J Menopausal Med (2015)

Bottom Line: Male C57BL/6 mice aged 5 weeks were raised for 13 weeks.There was no significant difference in VDR reactivity in seminal vesicles, body weight of rat and weight of seminal vesicles between HC group and normal control group.But we confirmed the VDR expression in seminal vesicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Korea.

ABSTRACT

Objectives: Genomic function of vitamin D receptor (VDR) indicates spermatogenesis that is important for in male reproductive organ authors evaluated the VDR expression in seminal vesicles with high cholesterol (HC) formula diet rat, because there is no report about relationship or difference in VDR in seminal vesicles between HC and control.

Methods: Male C57BL/6 mice aged 5 weeks were raised for 13 weeks. After one week of adaptation-period, they were fed different diet on normal AIN-93G diet, or HC diet containing 2% cholesterol for 12 weeks. The antibodies used were rabbit anti-VDR primary polyclonal.

Results: There was no significant difference in VDR reactivity in seminal vesicles, body weight of rat and weight of seminal vesicles between HC group and normal control group.

Conclusion: Our data give the no difference in expression of VDR of seminal vesicles rat between HC formula diet and normal AIN-93G diet. But we confirmed the VDR expression in seminal vesicles.

No MeSH data available.


Related in: MedlinePlus

Localization of vitamin D receptor in the seminal vesicle of each of groups as brown color. Immunohistochemistry was performed as described in the Material and Methods section. (A) normal control group (NC), (B, C) high cholesterol (HC) group, magnification: × 100. NC and diet on normal AIN-93G diet, HC diet on HC.
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Figure 1: Localization of vitamin D receptor in the seminal vesicle of each of groups as brown color. Immunohistochemistry was performed as described in the Material and Methods section. (A) normal control group (NC), (B, C) high cholesterol (HC) group, magnification: × 100. NC and diet on normal AIN-93G diet, HC diet on HC.

Mentions: The results of VDR have been described in Figure 1 as IHC. Epithelial cells of seminal vesicle showed reactivity in the nucleus with weak stain in cytoplasm (Fig. 1).


Expression of Vitamin D Receptor in Seminal Vesicles of Cholesterol Formula Mice.

Kim TH, Lee HH, Kim JM, Choi SD, Lee A, Hwang SY, Kim M, Kim YS, Yeom S - J Menopausal Med (2015)

Localization of vitamin D receptor in the seminal vesicle of each of groups as brown color. Immunohistochemistry was performed as described in the Material and Methods section. (A) normal control group (NC), (B, C) high cholesterol (HC) group, magnification: × 100. NC and diet on normal AIN-93G diet, HC diet on HC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561746&req=5

Figure 1: Localization of vitamin D receptor in the seminal vesicle of each of groups as brown color. Immunohistochemistry was performed as described in the Material and Methods section. (A) normal control group (NC), (B, C) high cholesterol (HC) group, magnification: × 100. NC and diet on normal AIN-93G diet, HC diet on HC.
Mentions: The results of VDR have been described in Figure 1 as IHC. Epithelial cells of seminal vesicle showed reactivity in the nucleus with weak stain in cytoplasm (Fig. 1).

Bottom Line: Male C57BL/6 mice aged 5 weeks were raised for 13 weeks.There was no significant difference in VDR reactivity in seminal vesicles, body weight of rat and weight of seminal vesicles between HC group and normal control group.But we confirmed the VDR expression in seminal vesicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Korea.

ABSTRACT

Objectives: Genomic function of vitamin D receptor (VDR) indicates spermatogenesis that is important for in male reproductive organ authors evaluated the VDR expression in seminal vesicles with high cholesterol (HC) formula diet rat, because there is no report about relationship or difference in VDR in seminal vesicles between HC and control.

Methods: Male C57BL/6 mice aged 5 weeks were raised for 13 weeks. After one week of adaptation-period, they were fed different diet on normal AIN-93G diet, or HC diet containing 2% cholesterol for 12 weeks. The antibodies used were rabbit anti-VDR primary polyclonal.

Results: There was no significant difference in VDR reactivity in seminal vesicles, body weight of rat and weight of seminal vesicles between HC group and normal control group.

Conclusion: Our data give the no difference in expression of VDR of seminal vesicles rat between HC formula diet and normal AIN-93G diet. But we confirmed the VDR expression in seminal vesicles.

No MeSH data available.


Related in: MedlinePlus