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The Enterobacterium Trabulsiella odontotermitis Presents Novel Adaptations Related to Its Association with Fungus-Growing Termites.

Sapountzis P, Gruntjes T, Otani S, Estevez J, da Costa RR, Plunkett G, Perna NT, Poulsen M - Appl. Environ. Microbiol. (2015)

Bottom Line: Fungus-growing termites rely on symbiotic microorganisms to help break down plant material and to obtain nutrients.Taking diverse approaches, we obtained a solid phylogenetic placement of T. odontotermitis among the Enterobacteriaceae, investigated the physiology and enzymatic profiles of T. odontotermitis isolates, determined the localization of the bacterium in the termite gut, compared draft genomes of two T. odontotermitis isolates to those of their close relatives, and examined the expression of genes relevant to host colonization and putative symbiont functions.Our findings support the hypothesis that T. odontotermitis is a facultative symbiont mainly located in the paunch compartment of the gut, with possible roles in carbohydrate metabolism and aflatoxin degradation, while displaying adaptations to association with the termite host, such as expressing genes for a type VI secretion system which has been demonstrated to assist bacterial competition, colonization, and survival within hosts.

View Article: PubMed Central - PubMed

Affiliation: Centre for Social Evolution, Department of Biology, University of Copenhagen, Copenhagen, Denmark Sapountzis@bio.ku.dk.

No MeSH data available.


Related in: MedlinePlus

Representative laser scanning confocal microscopy images of various gut tissues of Odontotermes soldiers. (A) Colon; (B) midgut; (C) paunch; (D) rectum. Trabulsiella bacteria can be seen as bright red/pink spots, while other bacteria are seen as bright blue spots and the insect cell nuclei are blue. The faint diffuse cytoplasmic blue (B and C) and red (B) areas, observed mostly in pictures of the colon and the paunch, represent nonspecific staining. Bars, 20 μm.
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Figure 3: Representative laser scanning confocal microscopy images of various gut tissues of Odontotermes soldiers. (A) Colon; (B) midgut; (C) paunch; (D) rectum. Trabulsiella bacteria can be seen as bright red/pink spots, while other bacteria are seen as bright blue spots and the insect cell nuclei are blue. The faint diffuse cytoplasmic blue (B and C) and red (B) areas, observed mostly in pictures of the colon and the paunch, represent nonspecific staining. Bars, 20 μm.

Mentions: FISH confocal microscopy of workers and soldiers of Macrotermes and Odontotermes colonies showed similar patterns, with Trabulsiella being present in highest abundance in the paunch and in the rectum and, to a lesser extent, in the colon (Fig. 3). While Trabulsiella was most abundant in the paunch, many other bacteria were abundant in the midgut and rectum (Fig. 3).


The Enterobacterium Trabulsiella odontotermitis Presents Novel Adaptations Related to Its Association with Fungus-Growing Termites.

Sapountzis P, Gruntjes T, Otani S, Estevez J, da Costa RR, Plunkett G, Perna NT, Poulsen M - Appl. Environ. Microbiol. (2015)

Representative laser scanning confocal microscopy images of various gut tissues of Odontotermes soldiers. (A) Colon; (B) midgut; (C) paunch; (D) rectum. Trabulsiella bacteria can be seen as bright red/pink spots, while other bacteria are seen as bright blue spots and the insect cell nuclei are blue. The faint diffuse cytoplasmic blue (B and C) and red (B) areas, observed mostly in pictures of the colon and the paunch, represent nonspecific staining. Bars, 20 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561680&req=5

Figure 3: Representative laser scanning confocal microscopy images of various gut tissues of Odontotermes soldiers. (A) Colon; (B) midgut; (C) paunch; (D) rectum. Trabulsiella bacteria can be seen as bright red/pink spots, while other bacteria are seen as bright blue spots and the insect cell nuclei are blue. The faint diffuse cytoplasmic blue (B and C) and red (B) areas, observed mostly in pictures of the colon and the paunch, represent nonspecific staining. Bars, 20 μm.
Mentions: FISH confocal microscopy of workers and soldiers of Macrotermes and Odontotermes colonies showed similar patterns, with Trabulsiella being present in highest abundance in the paunch and in the rectum and, to a lesser extent, in the colon (Fig. 3). While Trabulsiella was most abundant in the paunch, many other bacteria were abundant in the midgut and rectum (Fig. 3).

Bottom Line: Fungus-growing termites rely on symbiotic microorganisms to help break down plant material and to obtain nutrients.Taking diverse approaches, we obtained a solid phylogenetic placement of T. odontotermitis among the Enterobacteriaceae, investigated the physiology and enzymatic profiles of T. odontotermitis isolates, determined the localization of the bacterium in the termite gut, compared draft genomes of two T. odontotermitis isolates to those of their close relatives, and examined the expression of genes relevant to host colonization and putative symbiont functions.Our findings support the hypothesis that T. odontotermitis is a facultative symbiont mainly located in the paunch compartment of the gut, with possible roles in carbohydrate metabolism and aflatoxin degradation, while displaying adaptations to association with the termite host, such as expressing genes for a type VI secretion system which has been demonstrated to assist bacterial competition, colonization, and survival within hosts.

View Article: PubMed Central - PubMed

Affiliation: Centre for Social Evolution, Department of Biology, University of Copenhagen, Copenhagen, Denmark Sapountzis@bio.ku.dk.

No MeSH data available.


Related in: MedlinePlus