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Surgery plus anesthesia induces loss of attention in mice.

Ren Q, Peng M, Dong Y, Zhang Y, Chen M, Yin N, Marcantonio ER, Xie Z - Front Cell Neurosci (2015)

Bottom Line: There is a need to develop animal models to study postoperative delirium.We found that there was a loss of attention at 24, but not 12 or 48 h following the surgery plus anesthesia (49% ± 5 vs. 33% ± 2.9, P = 0.011, N = 12) in the mice without significantly affecting the speed of their movements.These findings suggested that the surgery plus isoflurane anesthesia might induce behavioral and biochemical/cellular changes associated with delirium.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesia, Zhongda Hospital, Southeast University Nanjing, China ; Geriatric Anesthesia Research Unit, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School Charlestown, MA, USA.

ABSTRACT
There is a need to develop animal models to study postoperative delirium. Inattention is one of the symptoms of delirium. Increases in the levels of α-synuclein and S100β have been reported to be associated with delirium. Therefore, we set out to determine the effects of surgery plus general anesthesia on the behavioral changes (including loss of attention) in mice and on the levels of α-synuclein and S100β in the brain tissues of these mice. C57BL/6J mice (2- to 8-months-old) had a simple laparotomy plus isoflurane anesthesia. The behavioral changes, including attention level and the speed of movements, were determined 12, 24, and 48 h after the surgery plus anesthesia in the mice. The levels of α-synuclein and S100β in the cortex of these mice following the surgery plus anesthesia were determined by Western blot analysis. We found that there was a loss of attention at 24, but not 12 or 48 h following the surgery plus anesthesia (49% ± 5 vs. 33% ± 2.9, P = 0.011, N = 12) in the mice without significantly affecting the speed of their movements. There were increases in the levels of total α-synuclein (139% ± 33.5 vs. 100% ± 13.7, P = 0.037, N = 6) and S100β (142% ± 7.7 vs. 100% ± 6, P = 0.002, N = 6) in the cortex of the mice 12 h following the surgery plus anesthesia. These findings suggested that the surgery plus isoflurane anesthesia might induce behavioral and biochemical/cellular changes associated with delirium. We could use the surgery plus anesthesia in mice to develop an animal model to study postoperative delirium.

No MeSH data available.


Related in: MedlinePlus

Surgery plus anesthesia decreases the attention level at 24, but not 12 or 48 h after the surgery plus anesthesia in mice. (A) Surgery plus anesthesia (black bar) does not decrease the attention level as compared to the sham condition (white bar) in mice 12 h after the surgery plus anesthesia. (B) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 12 h after the surgery plus anesthesia. (C) Surgery plus anesthesia (black bar) decreases the attention level as compared to the sham condition (white bar) in mice 24 h after the surgery plus anesthesia. (D) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 24 h after the surgery plus anesthesia. (E) Surgery plus anesthesia (black bar) does not decrease the attention level as compared to the sham condition (white bar) in mice 48 h after the surgery plus anesthesia. (F) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 48 h after the surgery plus anesthesia. N = 12 in the control condition group and N = 12 in the surgery plus anesthesia group. *P < 0.05.
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Figure 1: Surgery plus anesthesia decreases the attention level at 24, but not 12 or 48 h after the surgery plus anesthesia in mice. (A) Surgery plus anesthesia (black bar) does not decrease the attention level as compared to the sham condition (white bar) in mice 12 h after the surgery plus anesthesia. (B) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 12 h after the surgery plus anesthesia. (C) Surgery plus anesthesia (black bar) decreases the attention level as compared to the sham condition (white bar) in mice 24 h after the surgery plus anesthesia. (D) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 24 h after the surgery plus anesthesia. (E) Surgery plus anesthesia (black bar) does not decrease the attention level as compared to the sham condition (white bar) in mice 48 h after the surgery plus anesthesia. (F) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 48 h after the surgery plus anesthesia. N = 12 in the control condition group and N = 12 in the surgery plus anesthesia group. *P < 0.05.

Mentions: We assessed whether surgery plus anesthesia could affect the behavioral changes including attention level in the mice. The mice had open abdominal surgery plus isoflurane anesthesia as described in the Materials and Methods Section. Then, we measured the effects of the surgery plus anesthesia on attention level 12 (Figures 1A,B), 24 (Figures 1C,D) and 48 (Figures 1E,F) h after the surgery plus anesthesia. As can be seen in Figure 1, the surgery plus anesthesia (black bar, Figure 1A) did not significantly alter the attention level of the mice compared to the sham condition (white bar, Figure 1A) 12 h after the surgery plus anesthesia (Figure 1A, P = 0.588, Student's t-test, N = 12 in each group). The surgery plus anesthesia did not significantly alter the speed of the mice's movements 12 h after the surgery plus anesthesia (Figure 1B, P = 0.290, Student's t-test, N = 12 in each group).


Surgery plus anesthesia induces loss of attention in mice.

Ren Q, Peng M, Dong Y, Zhang Y, Chen M, Yin N, Marcantonio ER, Xie Z - Front Cell Neurosci (2015)

Surgery plus anesthesia decreases the attention level at 24, but not 12 or 48 h after the surgery plus anesthesia in mice. (A) Surgery plus anesthesia (black bar) does not decrease the attention level as compared to the sham condition (white bar) in mice 12 h after the surgery plus anesthesia. (B) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 12 h after the surgery plus anesthesia. (C) Surgery plus anesthesia (black bar) decreases the attention level as compared to the sham condition (white bar) in mice 24 h after the surgery plus anesthesia. (D) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 24 h after the surgery plus anesthesia. (E) Surgery plus anesthesia (black bar) does not decrease the attention level as compared to the sham condition (white bar) in mice 48 h after the surgery plus anesthesia. (F) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 48 h after the surgery plus anesthesia. N = 12 in the control condition group and N = 12 in the surgery plus anesthesia group. *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4561675&req=5

Figure 1: Surgery plus anesthesia decreases the attention level at 24, but not 12 or 48 h after the surgery plus anesthesia in mice. (A) Surgery plus anesthesia (black bar) does not decrease the attention level as compared to the sham condition (white bar) in mice 12 h after the surgery plus anesthesia. (B) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 12 h after the surgery plus anesthesia. (C) Surgery plus anesthesia (black bar) decreases the attention level as compared to the sham condition (white bar) in mice 24 h after the surgery plus anesthesia. (D) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 24 h after the surgery plus anesthesia. (E) Surgery plus anesthesia (black bar) does not decrease the attention level as compared to the sham condition (white bar) in mice 48 h after the surgery plus anesthesia. (F) Surgery plus anesthesia (black bar) does not decrease the speed of movement as compared to the sham condition (white bar) in mice 48 h after the surgery plus anesthesia. N = 12 in the control condition group and N = 12 in the surgery plus anesthesia group. *P < 0.05.
Mentions: We assessed whether surgery plus anesthesia could affect the behavioral changes including attention level in the mice. The mice had open abdominal surgery plus isoflurane anesthesia as described in the Materials and Methods Section. Then, we measured the effects of the surgery plus anesthesia on attention level 12 (Figures 1A,B), 24 (Figures 1C,D) and 48 (Figures 1E,F) h after the surgery plus anesthesia. As can be seen in Figure 1, the surgery plus anesthesia (black bar, Figure 1A) did not significantly alter the attention level of the mice compared to the sham condition (white bar, Figure 1A) 12 h after the surgery plus anesthesia (Figure 1A, P = 0.588, Student's t-test, N = 12 in each group). The surgery plus anesthesia did not significantly alter the speed of the mice's movements 12 h after the surgery plus anesthesia (Figure 1B, P = 0.290, Student's t-test, N = 12 in each group).

Bottom Line: There is a need to develop animal models to study postoperative delirium.We found that there was a loss of attention at 24, but not 12 or 48 h following the surgery plus anesthesia (49% ± 5 vs. 33% ± 2.9, P = 0.011, N = 12) in the mice without significantly affecting the speed of their movements.These findings suggested that the surgery plus isoflurane anesthesia might induce behavioral and biochemical/cellular changes associated with delirium.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesia, Zhongda Hospital, Southeast University Nanjing, China ; Geriatric Anesthesia Research Unit, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School Charlestown, MA, USA.

ABSTRACT
There is a need to develop animal models to study postoperative delirium. Inattention is one of the symptoms of delirium. Increases in the levels of α-synuclein and S100β have been reported to be associated with delirium. Therefore, we set out to determine the effects of surgery plus general anesthesia on the behavioral changes (including loss of attention) in mice and on the levels of α-synuclein and S100β in the brain tissues of these mice. C57BL/6J mice (2- to 8-months-old) had a simple laparotomy plus isoflurane anesthesia. The behavioral changes, including attention level and the speed of movements, were determined 12, 24, and 48 h after the surgery plus anesthesia in the mice. The levels of α-synuclein and S100β in the cortex of these mice following the surgery plus anesthesia were determined by Western blot analysis. We found that there was a loss of attention at 24, but not 12 or 48 h following the surgery plus anesthesia (49% ± 5 vs. 33% ± 2.9, P = 0.011, N = 12) in the mice without significantly affecting the speed of their movements. There were increases in the levels of total α-synuclein (139% ± 33.5 vs. 100% ± 13.7, P = 0.037, N = 6) and S100β (142% ± 7.7 vs. 100% ± 6, P = 0.002, N = 6) in the cortex of the mice 12 h following the surgery plus anesthesia. These findings suggested that the surgery plus isoflurane anesthesia might induce behavioral and biochemical/cellular changes associated with delirium. We could use the surgery plus anesthesia in mice to develop an animal model to study postoperative delirium.

No MeSH data available.


Related in: MedlinePlus