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Ongoing ecological speciation in Cotesia sesamiae, a biological control agent of cereal stem borers.

Kaiser L, Le Ru BP, Kaoula F, Paillusson C, Capdevielle-Dulac C, Obonyo JO, Herniou EA, Jancek S, Branca A, Calatayud PA, Silvain JF, Dupas S - Evol Appl (2015)

Bottom Line: To develop efficient and safe biological control, we need to reliably identify natural enemy species, determine their host range, and understand the mechanisms that drive host range evolution.We found that one highly supported lineage showed all the hallmarks of a cryptic species.It is associated with one host insect, Sesamia nonagrioides, and is reproductively isolated from the other two lineages by pre- and postmating barriers.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire Evolution, Génomes, Comportement et Ecologie, UMR CNRS-Univ. Paris-Sud-IRD, Univ. Paris-Saclay Gif-sur-Yvette Cedex, France ; INRA, UMR 1392, Institut d'Ecologie et des Sciences de l'Environnement de Paris Paris, France.

ABSTRACT
To develop efficient and safe biological control, we need to reliably identify natural enemy species, determine their host range, and understand the mechanisms that drive host range evolution. We investigated these points in Cotesia sesamiae, an African parasitic wasp of cereal stem borers. Phylogenetic analyses of 74 individual wasps, based on six mitochondrial and nuclear genes, revealed three lineages. We then investigated the ecological status (host plant and host insect ranges in the field, and host insect suitability tests) and the biological status (cross-mating tests) of the three lineages. We found that one highly supported lineage showed all the hallmarks of a cryptic species. It is associated with one host insect, Sesamia nonagrioides, and is reproductively isolated from the other two lineages by pre- and postmating barriers. The other two lineages had a more variable phylogenetic support, depending on the set of genes; they exhibited an overlapping and diversified range of host species and are not reproductively isolated from one another. We discuss the ecological conditions and mechanisms that likely generated this ongoing speciation and the relevance of this new specialist taxon in the genus Cotesia for biological control.

No MeSH data available.


Phylogeny of Cotesia sesamiae individuals and relatives based on concatenated mtDNA of 3 genes (CO1, 16S, and NADH) and nDNA of a nonviral (LWRH) and two viral genes (EP2 and histone) in relation to host insect and host plant species matrix. See Materials and methods for substitution model selection with PartitionFinder and phylogenetic tree inference in Mr Bayes. Posterior probabilities are given at nodes. All samples have a reference code corresponding to the data bank of the Laboratoire Evolution, Génomes, Comportement et Ecologie. Insect family: Cr, Crambidae; No, Noctuidae. Plant family: Po, Poaceae; Ty, Typhaceae; Cy, Cyperaceae.
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fig01: Phylogeny of Cotesia sesamiae individuals and relatives based on concatenated mtDNA of 3 genes (CO1, 16S, and NADH) and nDNA of a nonviral (LWRH) and two viral genes (EP2 and histone) in relation to host insect and host plant species matrix. See Materials and methods for substitution model selection with PartitionFinder and phylogenetic tree inference in Mr Bayes. Posterior probabilities are given at nodes. All samples have a reference code corresponding to the data bank of the Laboratoire Evolution, Génomes, Comportement et Ecologie. Insect family: Cr, Crambidae; No, Noctuidae. Plant family: Po, Poaceae; Ty, Typhaceae; Cy, Cyperaceae.

Mentions: Phylogenetic reconstructions obtained from the all-gene dataset, or for mtDNA+LWRH or PVD genes independently, strongly supported the monophyly of the three sister species in the C. flavipes complex, with posterior probability ranging from 0.98 to 1 and evidenced the relationship C. flavipes (C. chilonis, C. sesamiae) (Fig. 1: mtDNA+LWRH+PDV; Fig. S1A: mtDNA+LWRH; Fig. S1B: PDV). The mtDNA genes provided a lower support to the C. sesamiae lineage, and the LWRH gene failed to resolve relationships within the flavipes complex (Table 1).


Ongoing ecological speciation in Cotesia sesamiae, a biological control agent of cereal stem borers.

Kaiser L, Le Ru BP, Kaoula F, Paillusson C, Capdevielle-Dulac C, Obonyo JO, Herniou EA, Jancek S, Branca A, Calatayud PA, Silvain JF, Dupas S - Evol Appl (2015)

Phylogeny of Cotesia sesamiae individuals and relatives based on concatenated mtDNA of 3 genes (CO1, 16S, and NADH) and nDNA of a nonviral (LWRH) and two viral genes (EP2 and histone) in relation to host insect and host plant species matrix. See Materials and methods for substitution model selection with PartitionFinder and phylogenetic tree inference in Mr Bayes. Posterior probabilities are given at nodes. All samples have a reference code corresponding to the data bank of the Laboratoire Evolution, Génomes, Comportement et Ecologie. Insect family: Cr, Crambidae; No, Noctuidae. Plant family: Po, Poaceae; Ty, Typhaceae; Cy, Cyperaceae.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4561570&req=5

fig01: Phylogeny of Cotesia sesamiae individuals and relatives based on concatenated mtDNA of 3 genes (CO1, 16S, and NADH) and nDNA of a nonviral (LWRH) and two viral genes (EP2 and histone) in relation to host insect and host plant species matrix. See Materials and methods for substitution model selection with PartitionFinder and phylogenetic tree inference in Mr Bayes. Posterior probabilities are given at nodes. All samples have a reference code corresponding to the data bank of the Laboratoire Evolution, Génomes, Comportement et Ecologie. Insect family: Cr, Crambidae; No, Noctuidae. Plant family: Po, Poaceae; Ty, Typhaceae; Cy, Cyperaceae.
Mentions: Phylogenetic reconstructions obtained from the all-gene dataset, or for mtDNA+LWRH or PVD genes independently, strongly supported the monophyly of the three sister species in the C. flavipes complex, with posterior probability ranging from 0.98 to 1 and evidenced the relationship C. flavipes (C. chilonis, C. sesamiae) (Fig. 1: mtDNA+LWRH+PDV; Fig. S1A: mtDNA+LWRH; Fig. S1B: PDV). The mtDNA genes provided a lower support to the C. sesamiae lineage, and the LWRH gene failed to resolve relationships within the flavipes complex (Table 1).

Bottom Line: To develop efficient and safe biological control, we need to reliably identify natural enemy species, determine their host range, and understand the mechanisms that drive host range evolution.We found that one highly supported lineage showed all the hallmarks of a cryptic species.It is associated with one host insect, Sesamia nonagrioides, and is reproductively isolated from the other two lineages by pre- and postmating barriers.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire Evolution, Génomes, Comportement et Ecologie, UMR CNRS-Univ. Paris-Sud-IRD, Univ. Paris-Saclay Gif-sur-Yvette Cedex, France ; INRA, UMR 1392, Institut d'Ecologie et des Sciences de l'Environnement de Paris Paris, France.

ABSTRACT
To develop efficient and safe biological control, we need to reliably identify natural enemy species, determine their host range, and understand the mechanisms that drive host range evolution. We investigated these points in Cotesia sesamiae, an African parasitic wasp of cereal stem borers. Phylogenetic analyses of 74 individual wasps, based on six mitochondrial and nuclear genes, revealed three lineages. We then investigated the ecological status (host plant and host insect ranges in the field, and host insect suitability tests) and the biological status (cross-mating tests) of the three lineages. We found that one highly supported lineage showed all the hallmarks of a cryptic species. It is associated with one host insect, Sesamia nonagrioides, and is reproductively isolated from the other two lineages by pre- and postmating barriers. The other two lineages had a more variable phylogenetic support, depending on the set of genes; they exhibited an overlapping and diversified range of host species and are not reproductively isolated from one another. We discuss the ecological conditions and mechanisms that likely generated this ongoing speciation and the relevance of this new specialist taxon in the genus Cotesia for biological control.

No MeSH data available.