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The anti-inflammatory and antifibrotic effects of Coreopsis tinctoria Nutt on high-glucose-fat diet and streptozotocin-induced diabetic renal damage in rats.

Yao L, Li L, Li X, Li H, Zhang Y, Zhang R, Wang J, Mao X - BMC Complement Altern Med (2015)

Bottom Line: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis.However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1.AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-β1/Smad signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: College of Traditional Chinese Medicine, Xinjiang Medical University, Xinyi Street 393, Urumuqi, 830011, China. ylyl1026@sina.com.

ABSTRACT

Background: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis. Coreopsis tinctoria Nutt, traditionally used as a healthcare tea, has anti-inflammatory, anti-hyperlipidemia, and glycemic regulation activities. The aim of our study was to investigate the renal protective effect of ethyl acetate extract of C. tinctoria Nutt (AC) on high-glucose-fat diet and streptozotocin (STZ)-induced diabetic rats.

Methods: A diabetic rat model was induced by high-glucose-fat diet and intraperitoneal injection of 35 mg/kg STZ. After treatment with AC at a daily dose of 150, 300 or, 600 mg/kg for 4 weeks, metabolic and renal function parameters of serum and urine were examined. Degree of renal damage, renal proinflammatory cytokines, and fibrotic protein expression were analyzed by histopathology and immunohistochemistry. Renal AMP-activated protein kinase (AMPK) and transforming growth factor (TGF)-β1/Smad signaling pathway were determined by western blotting.

Results: Diabetic rats showed obvious renal dysfunction, inflammation and fibrosis. However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1. AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-β1/Smad signaling pathway. Meanwhile, AMPKα as a protective cytokine was markedly stimulated by AC.

Conclusion: In summary, AC controls blood glucose, inhibits inflammatory and fibrotic processes, suppresses the TGF-β1/Smad signaling pathway, and activates phosphorylation of AMPKα in the kidneys, which confirms the protective effects of AC in the early stage of diabetic kidney disease.

No MeSH data available.


Related in: MedlinePlus

Expression of TGF-β1, MCP-1 and ICAM-1 in kidney. a Immunohistochemistry of TGF-β1, MCP-1 and ICAM-1 (orginal magnification 400×). b TGF-β1, MCP-1 and ICAM-1 expression was semiquantitatively analyzed. Values were expressed as mean ± SEM (n = 4). #P < 0.01 versus normal group; *P < 0.05 versus control group; **P < 0.01 versus control group
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Fig3: Expression of TGF-β1, MCP-1 and ICAM-1 in kidney. a Immunohistochemistry of TGF-β1, MCP-1 and ICAM-1 (orginal magnification 400×). b TGF-β1, MCP-1 and ICAM-1 expression was semiquantitatively analyzed. Values were expressed as mean ± SEM (n = 4). #P < 0.01 versus normal group; *P < 0.05 versus control group; **P < 0.01 versus control group

Mentions: To investigate the anti-inflammatory and anti-fibrotic effects of AC, immunohistochemistry was used to determine protein expression of TGF-β1, MCP-1, ICAM-1, collagen IV and fibronectin in diabetic kidney. Positive expression was stained brown–yellow (Figs. 3a and 4a). Compared with the NC group, the DC group showed significant overexpression of the five cytokines (Figs. 3b and 4b; P < 0.01). All cytokine expression was decreased to some extent after AC and metformin treatment. AC 600 mg/kg had the most inhibitory effect of protein expression of TGF-β1, MCP-1, ICAM-1, collagen IV and fibronectin. These results indicate that AC has a protective effect on diabetic renal inflammation and fibrosis.Fig. 3


The anti-inflammatory and antifibrotic effects of Coreopsis tinctoria Nutt on high-glucose-fat diet and streptozotocin-induced diabetic renal damage in rats.

Yao L, Li L, Li X, Li H, Zhang Y, Zhang R, Wang J, Mao X - BMC Complement Altern Med (2015)

Expression of TGF-β1, MCP-1 and ICAM-1 in kidney. a Immunohistochemistry of TGF-β1, MCP-1 and ICAM-1 (orginal magnification 400×). b TGF-β1, MCP-1 and ICAM-1 expression was semiquantitatively analyzed. Values were expressed as mean ± SEM (n = 4). #P < 0.01 versus normal group; *P < 0.05 versus control group; **P < 0.01 versus control group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4561427&req=5

Fig3: Expression of TGF-β1, MCP-1 and ICAM-1 in kidney. a Immunohistochemistry of TGF-β1, MCP-1 and ICAM-1 (orginal magnification 400×). b TGF-β1, MCP-1 and ICAM-1 expression was semiquantitatively analyzed. Values were expressed as mean ± SEM (n = 4). #P < 0.01 versus normal group; *P < 0.05 versus control group; **P < 0.01 versus control group
Mentions: To investigate the anti-inflammatory and anti-fibrotic effects of AC, immunohistochemistry was used to determine protein expression of TGF-β1, MCP-1, ICAM-1, collagen IV and fibronectin in diabetic kidney. Positive expression was stained brown–yellow (Figs. 3a and 4a). Compared with the NC group, the DC group showed significant overexpression of the five cytokines (Figs. 3b and 4b; P < 0.01). All cytokine expression was decreased to some extent after AC and metformin treatment. AC 600 mg/kg had the most inhibitory effect of protein expression of TGF-β1, MCP-1, ICAM-1, collagen IV and fibronectin. These results indicate that AC has a protective effect on diabetic renal inflammation and fibrosis.Fig. 3

Bottom Line: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis.However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1.AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-β1/Smad signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: College of Traditional Chinese Medicine, Xinjiang Medical University, Xinyi Street 393, Urumuqi, 830011, China. ylyl1026@sina.com.

ABSTRACT

Background: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis. Coreopsis tinctoria Nutt, traditionally used as a healthcare tea, has anti-inflammatory, anti-hyperlipidemia, and glycemic regulation activities. The aim of our study was to investigate the renal protective effect of ethyl acetate extract of C. tinctoria Nutt (AC) on high-glucose-fat diet and streptozotocin (STZ)-induced diabetic rats.

Methods: A diabetic rat model was induced by high-glucose-fat diet and intraperitoneal injection of 35 mg/kg STZ. After treatment with AC at a daily dose of 150, 300 or, 600 mg/kg for 4 weeks, metabolic and renal function parameters of serum and urine were examined. Degree of renal damage, renal proinflammatory cytokines, and fibrotic protein expression were analyzed by histopathology and immunohistochemistry. Renal AMP-activated protein kinase (AMPK) and transforming growth factor (TGF)-β1/Smad signaling pathway were determined by western blotting.

Results: Diabetic rats showed obvious renal dysfunction, inflammation and fibrosis. However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1. AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-β1/Smad signaling pathway. Meanwhile, AMPKα as a protective cytokine was markedly stimulated by AC.

Conclusion: In summary, AC controls blood glucose, inhibits inflammatory and fibrotic processes, suppresses the TGF-β1/Smad signaling pathway, and activates phosphorylation of AMPKα in the kidneys, which confirms the protective effects of AC in the early stage of diabetic kidney disease.

No MeSH data available.


Related in: MedlinePlus