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The anti-inflammatory and antifibrotic effects of Coreopsis tinctoria Nutt on high-glucose-fat diet and streptozotocin-induced diabetic renal damage in rats.

Yao L, Li L, Li X, Li H, Zhang Y, Zhang R, Wang J, Mao X - BMC Complement Altern Med (2015)

Bottom Line: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis.However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1.AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-β1/Smad signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: College of Traditional Chinese Medicine, Xinjiang Medical University, Xinyi Street 393, Urumuqi, 830011, China. ylyl1026@sina.com.

ABSTRACT

Background: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis. Coreopsis tinctoria Nutt, traditionally used as a healthcare tea, has anti-inflammatory, anti-hyperlipidemia, and glycemic regulation activities. The aim of our study was to investigate the renal protective effect of ethyl acetate extract of C. tinctoria Nutt (AC) on high-glucose-fat diet and streptozotocin (STZ)-induced diabetic rats.

Methods: A diabetic rat model was induced by high-glucose-fat diet and intraperitoneal injection of 35 mg/kg STZ. After treatment with AC at a daily dose of 150, 300 or, 600 mg/kg for 4 weeks, metabolic and renal function parameters of serum and urine were examined. Degree of renal damage, renal proinflammatory cytokines, and fibrotic protein expression were analyzed by histopathology and immunohistochemistry. Renal AMP-activated protein kinase (AMPK) and transforming growth factor (TGF)-β1/Smad signaling pathway were determined by western blotting.

Results: Diabetic rats showed obvious renal dysfunction, inflammation and fibrosis. However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1. AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-β1/Smad signaling pathway. Meanwhile, AMPKα as a protective cytokine was markedly stimulated by AC.

Conclusion: In summary, AC controls blood glucose, inhibits inflammatory and fibrotic processes, suppresses the TGF-β1/Smad signaling pathway, and activates phosphorylation of AMPKα in the kidneys, which confirms the protective effects of AC in the early stage of diabetic kidney disease.

No MeSH data available.


Related in: MedlinePlus

Renal histology after treatment with or without AC and metformin. a Pathological features of the left kidney tissue stained with H&E and Masson stain (orginal magnification 400×). Each photomicrograph represented the pathological feature of four rats from each group. b Matrix expansion and area of fibrosis among the groups were semiquantitatively analyzed. Values were expressed as mean ± SEM (n = 4). #P < 0.01 versus normal group; *P < 0.05 versus control group; **P < 0.01 versus control group
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Fig2: Renal histology after treatment with or without AC and metformin. a Pathological features of the left kidney tissue stained with H&E and Masson stain (orginal magnification 400×). Each photomicrograph represented the pathological feature of four rats from each group. b Matrix expansion and area of fibrosis among the groups were semiquantitatively analyzed. Values were expressed as mean ± SEM (n = 4). #P < 0.01 versus normal group; *P < 0.05 versus control group; **P < 0.01 versus control group

Mentions: H&E and Masson’s stain were used to detect renal histology characteristics. Judging from H&E staining, glomerular and tubular lesions in the normal group were not obvious. The DC group showed a dramatic increase in mesangial matrix and basement membrane thickening, both in glomerular and tubular lesions (Fig. 2a). There was some improvement after treatment with 150 mg/kg AC (P < 0.05), 300 mg/kg AC (P < 0.05), 600 mg/kg AC (P < 0.01) and 200 mg/kg metformin (P < 0.05). Collagen deposition was determined by Masson’s stain. Heavy collagen deposition was clearly observed in the DC group. This abnormality was ameliorated after treatment with AC (150 mg/kg, P < 0.05; 300 mg/kg, P < 0.05; and 600 mg/kg, P < 0.01) and metformin (200 mg/kg, P < 0.01). These results confirmed the protective effect of AC and metformin on diabetic renal damage.Fig. 2


The anti-inflammatory and antifibrotic effects of Coreopsis tinctoria Nutt on high-glucose-fat diet and streptozotocin-induced diabetic renal damage in rats.

Yao L, Li L, Li X, Li H, Zhang Y, Zhang R, Wang J, Mao X - BMC Complement Altern Med (2015)

Renal histology after treatment with or without AC and metformin. a Pathological features of the left kidney tissue stained with H&E and Masson stain (orginal magnification 400×). Each photomicrograph represented the pathological feature of four rats from each group. b Matrix expansion and area of fibrosis among the groups were semiquantitatively analyzed. Values were expressed as mean ± SEM (n = 4). #P < 0.01 versus normal group; *P < 0.05 versus control group; **P < 0.01 versus control group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4561427&req=5

Fig2: Renal histology after treatment with or without AC and metformin. a Pathological features of the left kidney tissue stained with H&E and Masson stain (orginal magnification 400×). Each photomicrograph represented the pathological feature of four rats from each group. b Matrix expansion and area of fibrosis among the groups were semiquantitatively analyzed. Values were expressed as mean ± SEM (n = 4). #P < 0.01 versus normal group; *P < 0.05 versus control group; **P < 0.01 versus control group
Mentions: H&E and Masson’s stain were used to detect renal histology characteristics. Judging from H&E staining, glomerular and tubular lesions in the normal group were not obvious. The DC group showed a dramatic increase in mesangial matrix and basement membrane thickening, both in glomerular and tubular lesions (Fig. 2a). There was some improvement after treatment with 150 mg/kg AC (P < 0.05), 300 mg/kg AC (P < 0.05), 600 mg/kg AC (P < 0.01) and 200 mg/kg metformin (P < 0.05). Collagen deposition was determined by Masson’s stain. Heavy collagen deposition was clearly observed in the DC group. This abnormality was ameliorated after treatment with AC (150 mg/kg, P < 0.05; 300 mg/kg, P < 0.05; and 600 mg/kg, P < 0.01) and metformin (200 mg/kg, P < 0.01). These results confirmed the protective effect of AC and metformin on diabetic renal damage.Fig. 2

Bottom Line: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis.However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1.AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-β1/Smad signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: College of Traditional Chinese Medicine, Xinjiang Medical University, Xinyi Street 393, Urumuqi, 830011, China. ylyl1026@sina.com.

ABSTRACT

Background: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis. Coreopsis tinctoria Nutt, traditionally used as a healthcare tea, has anti-inflammatory, anti-hyperlipidemia, and glycemic regulation activities. The aim of our study was to investigate the renal protective effect of ethyl acetate extract of C. tinctoria Nutt (AC) on high-glucose-fat diet and streptozotocin (STZ)-induced diabetic rats.

Methods: A diabetic rat model was induced by high-glucose-fat diet and intraperitoneal injection of 35 mg/kg STZ. After treatment with AC at a daily dose of 150, 300 or, 600 mg/kg for 4 weeks, metabolic and renal function parameters of serum and urine were examined. Degree of renal damage, renal proinflammatory cytokines, and fibrotic protein expression were analyzed by histopathology and immunohistochemistry. Renal AMP-activated protein kinase (AMPK) and transforming growth factor (TGF)-β1/Smad signaling pathway were determined by western blotting.

Results: Diabetic rats showed obvious renal dysfunction, inflammation and fibrosis. However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1. AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-β1/Smad signaling pathway. Meanwhile, AMPKα as a protective cytokine was markedly stimulated by AC.

Conclusion: In summary, AC controls blood glucose, inhibits inflammatory and fibrotic processes, suppresses the TGF-β1/Smad signaling pathway, and activates phosphorylation of AMPKα in the kidneys, which confirms the protective effects of AC in the early stage of diabetic kidney disease.

No MeSH data available.


Related in: MedlinePlus