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Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.

Chen M, Meng H, Zhao Y, Chen F, Yu S - BMC Complement Altern Med (2015)

Bottom Line: Antitumor activities significantly increased in a dose-dependent manner.These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

View Article: PubMed Central - PubMed

Affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.

ABSTRACT

Background: In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).

Methods: Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.

Results: The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.

Conclusions: The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effects in vitro of three compounds and 5-fluorouracil (5-Fu) control against a CACO-2 cells, b HepG2 cells and c MCF-7 cells. GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride, EP: epicatechin. All results are the means ± SD (n = 3). *p < 0.05, statistically significant in comparison with control
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Fig6: Inhibitory effects in vitro of three compounds and 5-fluorouracil (5-Fu) control against a CACO-2 cells, b HepG2 cells and c MCF-7 cells. GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride, EP: epicatechin. All results are the means ± SD (n = 3). *p < 0.05, statistically significant in comparison with control

Mentions: The antitumor activities of five fractions against cancer cells are shown in Fig. 5. Among the five extraction fractions, the ethyl acetate fraction showed the highest inhibitory effect on CACO-2 (Fig. 5a), HepG2 (Fig. 5b) and MCF-7 (Fig. 5c) cell proliferation at the concentrations ranging from 25 to 400 μg/mL. The inhibitory effect was displayed in a dose-dependent manner. As shown in Fig. 6, the inhibitory rates of the ethyl acetate fraction on CACO-2, HepG2 and MCF-7 cells were higher than other fractions. The degree of antitumor activities of the ethyl acetate fractions showed that active compounds may be more concentrated in the ethyl acetate fraction than may be present in other fractions.Fig. 5


Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.

Chen M, Meng H, Zhao Y, Chen F, Yu S - BMC Complement Altern Med (2015)

Inhibitory effects in vitro of three compounds and 5-fluorouracil (5-Fu) control against a CACO-2 cells, b HepG2 cells and c MCF-7 cells. GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride, EP: epicatechin. All results are the means ± SD (n = 3). *p < 0.05, statistically significant in comparison with control
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4561420&req=5

Fig6: Inhibitory effects in vitro of three compounds and 5-fluorouracil (5-Fu) control against a CACO-2 cells, b HepG2 cells and c MCF-7 cells. GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride, EP: epicatechin. All results are the means ± SD (n = 3). *p < 0.05, statistically significant in comparison with control
Mentions: The antitumor activities of five fractions against cancer cells are shown in Fig. 5. Among the five extraction fractions, the ethyl acetate fraction showed the highest inhibitory effect on CACO-2 (Fig. 5a), HepG2 (Fig. 5b) and MCF-7 (Fig. 5c) cell proliferation at the concentrations ranging from 25 to 400 μg/mL. The inhibitory effect was displayed in a dose-dependent manner. As shown in Fig. 6, the inhibitory rates of the ethyl acetate fraction on CACO-2, HepG2 and MCF-7 cells were higher than other fractions. The degree of antitumor activities of the ethyl acetate fractions showed that active compounds may be more concentrated in the ethyl acetate fraction than may be present in other fractions.Fig. 5

Bottom Line: Antitumor activities significantly increased in a dose-dependent manner.These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

View Article: PubMed Central - PubMed

Affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.

ABSTRACT

Background: In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).

Methods: Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.

Results: The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.

Conclusions: The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

No MeSH data available.


Related in: MedlinePlus