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Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.

Chen M, Meng H, Zhao Y, Chen F, Yu S - BMC Complement Altern Med (2015)

Bottom Line: Antitumor activities significantly increased in a dose-dependent manner.These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

View Article: PubMed Central - PubMed

Affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.

ABSTRACT

Background: In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).

Methods: Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.

Results: The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.

Conclusions: The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

No MeSH data available.


Related in: MedlinePlus

The ORAC values of each fraction and compound isolated from SBM. a PEF: petroleum ether fraction; CF: chloroform fraction; EF: ethyl acetate fraction; BF: n-butanol fraction; WF: water fraction; b GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride; EP: epicatechin. All results are the means ± SD (n = 3). *p < 0.05, statistically significant in comparison with control
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Fig4: The ORAC values of each fraction and compound isolated from SBM. a PEF: petroleum ether fraction; CF: chloroform fraction; EF: ethyl acetate fraction; BF: n-butanol fraction; WF: water fraction; b GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride; EP: epicatechin. All results are the means ± SD (n = 3). *p < 0.05, statistically significant in comparison with control

Mentions: The oxygen radical absorbance capacities of samples were shown in Fig. 4. As shown in Fig. 4a, all the extraction fractions showed strong oxygen radical absorbance capacity. The ORAC values were in the decreasing order of ethyl acetate fraction > n-butanol fraction > water fraction > chloroform fraction > petroleum ether fraction. The ORAC values of three compounds are shown in Fig. 4b. GA showed stronger oxygen radical absorbance capacity than Vc while those of CGC and EP were relatively low.Fig. 4


Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.

Chen M, Meng H, Zhao Y, Chen F, Yu S - BMC Complement Altern Med (2015)

The ORAC values of each fraction and compound isolated from SBM. a PEF: petroleum ether fraction; CF: chloroform fraction; EF: ethyl acetate fraction; BF: n-butanol fraction; WF: water fraction; b GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride; EP: epicatechin. All results are the means ± SD (n = 3). *p < 0.05, statistically significant in comparison with control
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4561420&req=5

Fig4: The ORAC values of each fraction and compound isolated from SBM. a PEF: petroleum ether fraction; CF: chloroform fraction; EF: ethyl acetate fraction; BF: n-butanol fraction; WF: water fraction; b GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride; EP: epicatechin. All results are the means ± SD (n = 3). *p < 0.05, statistically significant in comparison with control
Mentions: The oxygen radical absorbance capacities of samples were shown in Fig. 4. As shown in Fig. 4a, all the extraction fractions showed strong oxygen radical absorbance capacity. The ORAC values were in the decreasing order of ethyl acetate fraction > n-butanol fraction > water fraction > chloroform fraction > petroleum ether fraction. The ORAC values of three compounds are shown in Fig. 4b. GA showed stronger oxygen radical absorbance capacity than Vc while those of CGC and EP were relatively low.Fig. 4

Bottom Line: Antitumor activities significantly increased in a dose-dependent manner.These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

View Article: PubMed Central - PubMed

Affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.

ABSTRACT

Background: In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).

Methods: Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.

Results: The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.

Conclusions: The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

No MeSH data available.


Related in: MedlinePlus