Limits...
Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.

Chen M, Meng H, Zhao Y, Chen F, Yu S - BMC Complement Altern Med (2015)

Bottom Line: Antitumor activities significantly increased in a dose-dependent manner.These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

View Article: PubMed Central - PubMed

Affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.

ABSTRACT

Background: In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).

Methods: Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.

Results: The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.

Conclusions: The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

No MeSH data available.


Related in: MedlinePlus

DPPH radical scavenging activities of each fraction and compound isolated from SBM. a PEF: petroleum ether fraction; CF: chloroform fraction; EF: ethyl acetate fraction; BF: n-butanol fraction; WF: water fraction; b GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride; EP: epicatechin All results are the means ± SD (n = 3)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4561420&req=5

Fig2: DPPH radical scavenging activities of each fraction and compound isolated from SBM. a PEF: petroleum ether fraction; CF: chloroform fraction; EF: ethyl acetate fraction; BF: n-butanol fraction; WF: water fraction; b GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride; EP: epicatechin All results are the means ± SD (n = 3)

Mentions: The DPPH radical scavenging activities of extractions, GA, CGC and EP were shown in Fig. 2. All fractions showed remarkable scavenging activity against DPPH radicals in a dose-dependent manner. The scavenging activity of the ethyl acetate fraction was higher than those of other fractions (Fig. 2a). GA showed higher scavenging activities against DPPH radicals than Vc. CGC and EP showed relatively lower scavenging activities (Fig. 2b).Fig. 2


Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.

Chen M, Meng H, Zhao Y, Chen F, Yu S - BMC Complement Altern Med (2015)

DPPH radical scavenging activities of each fraction and compound isolated from SBM. a PEF: petroleum ether fraction; CF: chloroform fraction; EF: ethyl acetate fraction; BF: n-butanol fraction; WF: water fraction; b GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride; EP: epicatechin All results are the means ± SD (n = 3)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4561420&req=5

Fig2: DPPH radical scavenging activities of each fraction and compound isolated from SBM. a PEF: petroleum ether fraction; CF: chloroform fraction; EF: ethyl acetate fraction; BF: n-butanol fraction; WF: water fraction; b GA: gallic acid, CGC: cyanidin-3-O-glucoside chloride; EP: epicatechin All results are the means ± SD (n = 3)
Mentions: The DPPH radical scavenging activities of extractions, GA, CGC and EP were shown in Fig. 2. All fractions showed remarkable scavenging activity against DPPH radicals in a dose-dependent manner. The scavenging activity of the ethyl acetate fraction was higher than those of other fractions (Fig. 2a). GA showed higher scavenging activities against DPPH radicals than Vc. CGC and EP showed relatively lower scavenging activities (Fig. 2b).Fig. 2

Bottom Line: Antitumor activities significantly increased in a dose-dependent manner.These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

View Article: PubMed Central - PubMed

Affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.

ABSTRACT

Background: In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).

Methods: Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.

Results: The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.

Conclusions: The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

No MeSH data available.


Related in: MedlinePlus