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Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.

Chen M, Meng H, Zhao Y, Chen F, Yu S - BMC Complement Altern Med (2015)

Bottom Line: Antitumor activities significantly increased in a dose-dependent manner.These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

View Article: PubMed Central - PubMed

Affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.

ABSTRACT

Background: In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).

Methods: Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.

Results: The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.

Conclusions: The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

No MeSH data available.


Related in: MedlinePlus

The extraction and separation procedure of sugar beet molasses
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Fig1: The extraction and separation procedure of sugar beet molasses

Mentions: The ethyl acetate fraction (70.0 g) was loaded onto a column (3.5 cm × 100 cm) of macroporous resin D101, and the column was stepwise eluted with water, 30, 50, 70, and 95 % ethanol at a flow rate of 10 mL/min to yield five sub-fractions. After HPLC analyses, 30 % ethanol sub-fraction was further purified using a Sephadex LH-20 column chromatography and eluted with methanol, followed by a semi-preparative HPLC eluting with methanol/water, to yield compound 1 (1293 mg), compound 2 (241 mg) and compound 3 (486 mg). The semi-preparative high performance liquid chromatography (HPLC) system consisted of a C18 column (RP18, 10 μm, 250 mm × 20 mm), a Waters 600 pump and a Waters 2998 Diode Array Detector (DAD) (Waters, Milford, MA, USA) analyzed at 35 °C. The mobile phase was 1 % acetic acid aqueous solution (A) and methanol (B) with a gradient program of 0–10 min, linear gradient 5–10 % B; 10–70 min, linear gradient 10–20 % B; 70–90 min, 20 % B isocratic; 90–130 min, linear gradient 20–40 % B; 130–140 min, linear gradient 40–100 % B and 140–180 min 100 % B isocratic elution at a flow rate of 15 mL/min. T 2 mL of samples were injected. Absorption wavelengths were set at 280 and 360 nm. The extraction and separation procedure of SBMP is shown in Fig. 1.Fig. 1


Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses.

Chen M, Meng H, Zhao Y, Chen F, Yu S - BMC Complement Altern Med (2015)

The extraction and separation procedure of sugar beet molasses
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4561420&req=5

Fig1: The extraction and separation procedure of sugar beet molasses
Mentions: The ethyl acetate fraction (70.0 g) was loaded onto a column (3.5 cm × 100 cm) of macroporous resin D101, and the column was stepwise eluted with water, 30, 50, 70, and 95 % ethanol at a flow rate of 10 mL/min to yield five sub-fractions. After HPLC analyses, 30 % ethanol sub-fraction was further purified using a Sephadex LH-20 column chromatography and eluted with methanol, followed by a semi-preparative HPLC eluting with methanol/water, to yield compound 1 (1293 mg), compound 2 (241 mg) and compound 3 (486 mg). The semi-preparative high performance liquid chromatography (HPLC) system consisted of a C18 column (RP18, 10 μm, 250 mm × 20 mm), a Waters 600 pump and a Waters 2998 Diode Array Detector (DAD) (Waters, Milford, MA, USA) analyzed at 35 °C. The mobile phase was 1 % acetic acid aqueous solution (A) and methanol (B) with a gradient program of 0–10 min, linear gradient 5–10 % B; 10–70 min, linear gradient 10–20 % B; 70–90 min, 20 % B isocratic; 90–130 min, linear gradient 20–40 % B; 130–140 min, linear gradient 40–100 % B and 140–180 min 100 % B isocratic elution at a flow rate of 15 mL/min. T 2 mL of samples were injected. Absorption wavelengths were set at 280 and 360 nm. The extraction and separation procedure of SBMP is shown in Fig. 1.Fig. 1

Bottom Line: Antitumor activities significantly increased in a dose-dependent manner.These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

View Article: PubMed Central - PubMed

Affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan road, Guangzhou, 510640, China.

ABSTRACT

Background: In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).

Methods: Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.

Results: The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13% against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.

Conclusions: The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.

No MeSH data available.


Related in: MedlinePlus