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Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles.

Yang WJ, Liu FC, Hsieh JS, Chen CH, Hsiao SY, Lin CS - Reprod. Biol. Endocrinol. (2015)

Bottom Line: Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001).Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01).There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu City, Taiwan. molinda11@yahoo.com.tw.

ABSTRACT

Background: To determine whether matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP-1 and TIMP-2) in human follicular fluid, have any relationships with oocyte maturation in vivo and subsequent fertilization during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles.

Methods: The follicular fluids were obtained from 150 female patients undergoing IVF/ICSI cycles and a total of 1504 oocytes were retrieved for analysis. MMP-2 and MMP-9 activities were measured using zymography assay. TIMP-1 and TIMP-2 concentrations were quantitatively assessed using enzyme-linked immunosorbent assay (ELISA).

Results: Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001). Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01). There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes. The TIMP-1 and TIMP-2 also showed no correlation with the oocyte maturation rate.

Conclusions: The level of gelatinase MMP-2 in human follicular fluid might be a reliable marker of mature oocytes during IVF/ICSI cycles. Furthermore, the MMP-2 expression has a strong association with higher fertilization rate. Further studies are needed to support this theory.

No MeSH data available.


Representative MMP-2 and MMP-9 zymography gel of human follicular fluids. Zymography assay was performed as described in the section of Materials and Methods. The gel was analyzed by 8 % SDS-PAGE and followed by Coomassie staining. There was related higher MMP-2 activity compared with MMP-9 activity detected in the follicular fluids. The zymography gel was loaded with 5 μl of samples diluted 5× in PBS to quantify proMMP-2 and proMMP-9 activity. The representative gel shows follicular fluids from 4 patients with 100 % MII oocytes (high oocyte maturity) and 4 patients with <100 % MII oocytes (low oocyte maturity), as well as one control lane with recombinant proMMP-2 and proMMP-9. MMP-9 (92 kDa) and MMP-2 (72 kDa) on the zymography gel were indicated
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Fig1: Representative MMP-2 and MMP-9 zymography gel of human follicular fluids. Zymography assay was performed as described in the section of Materials and Methods. The gel was analyzed by 8 % SDS-PAGE and followed by Coomassie staining. There was related higher MMP-2 activity compared with MMP-9 activity detected in the follicular fluids. The zymography gel was loaded with 5 μl of samples diluted 5× in PBS to quantify proMMP-2 and proMMP-9 activity. The representative gel shows follicular fluids from 4 patients with 100 % MII oocytes (high oocyte maturity) and 4 patients with <100 % MII oocytes (low oocyte maturity), as well as one control lane with recombinant proMMP-2 and proMMP-9. MMP-9 (92 kDa) and MMP-2 (72 kDa) on the zymography gel were indicated

Mentions: Representative MMP zymography assay of ovary follicular fluid was shown in Fig. 1. Almost of MMP-2 and MMP-9 detected in the sample of follicular fluids is latent form in the present study. Therefore, in this study, we could only detect and discuss the latent form of MMP-2 and MMP-9.Fig. 1


Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles.

Yang WJ, Liu FC, Hsieh JS, Chen CH, Hsiao SY, Lin CS - Reprod. Biol. Endocrinol. (2015)

Representative MMP-2 and MMP-9 zymography gel of human follicular fluids. Zymography assay was performed as described in the section of Materials and Methods. The gel was analyzed by 8 % SDS-PAGE and followed by Coomassie staining. There was related higher MMP-2 activity compared with MMP-9 activity detected in the follicular fluids. The zymography gel was loaded with 5 μl of samples diluted 5× in PBS to quantify proMMP-2 and proMMP-9 activity. The representative gel shows follicular fluids from 4 patients with 100 % MII oocytes (high oocyte maturity) and 4 patients with <100 % MII oocytes (low oocyte maturity), as well as one control lane with recombinant proMMP-2 and proMMP-9. MMP-9 (92 kDa) and MMP-2 (72 kDa) on the zymography gel were indicated
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4559921&req=5

Fig1: Representative MMP-2 and MMP-9 zymography gel of human follicular fluids. Zymography assay was performed as described in the section of Materials and Methods. The gel was analyzed by 8 % SDS-PAGE and followed by Coomassie staining. There was related higher MMP-2 activity compared with MMP-9 activity detected in the follicular fluids. The zymography gel was loaded with 5 μl of samples diluted 5× in PBS to quantify proMMP-2 and proMMP-9 activity. The representative gel shows follicular fluids from 4 patients with 100 % MII oocytes (high oocyte maturity) and 4 patients with <100 % MII oocytes (low oocyte maturity), as well as one control lane with recombinant proMMP-2 and proMMP-9. MMP-9 (92 kDa) and MMP-2 (72 kDa) on the zymography gel were indicated
Mentions: Representative MMP zymography assay of ovary follicular fluid was shown in Fig. 1. Almost of MMP-2 and MMP-9 detected in the sample of follicular fluids is latent form in the present study. Therefore, in this study, we could only detect and discuss the latent form of MMP-2 and MMP-9.Fig. 1

Bottom Line: Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001).Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01).There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu City, Taiwan. molinda11@yahoo.com.tw.

ABSTRACT

Background: To determine whether matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP-1 and TIMP-2) in human follicular fluid, have any relationships with oocyte maturation in vivo and subsequent fertilization during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles.

Methods: The follicular fluids were obtained from 150 female patients undergoing IVF/ICSI cycles and a total of 1504 oocytes were retrieved for analysis. MMP-2 and MMP-9 activities were measured using zymography assay. TIMP-1 and TIMP-2 concentrations were quantitatively assessed using enzyme-linked immunosorbent assay (ELISA).

Results: Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001). Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01). There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes. The TIMP-1 and TIMP-2 also showed no correlation with the oocyte maturation rate.

Conclusions: The level of gelatinase MMP-2 in human follicular fluid might be a reliable marker of mature oocytes during IVF/ICSI cycles. Furthermore, the MMP-2 expression has a strong association with higher fertilization rate. Further studies are needed to support this theory.

No MeSH data available.