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Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark.

Parvin MS, Das N, Jahan N, Akhter MA, Nahar L, Islam ME - BMC Res Notes (2015)

Bottom Line: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test.Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. rumiraj2@yahoo.com.

ABSTRACT

Background: The various parts of Cresecentia cujete have some important biological activities. In folklore medicine leaves are used to treat hematomas, tumors and hypertension. Fruit decoction is used to treat diarrhea, stomachaches, cold, bronchitis, cough, asthma, and urethritis. The present study was designed to explore the anti-inflammatory and antibacterial potential of C. cujete leaves and stem bark. Anti-inflammatory activity was evaluated by in vitro human red blood cell (HRBC) membrane stabilization method and antibacterial activity by disc diffusion method.

Methods: In vitro anti-inflammatory activity was evaluated by human red blood cell (HRBC) membrane stabilization method while in vitro antibacterial activity was evaluated using cultures of Escherichia coli and Staphylococcus aureus by disc diffusion method. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined by Folin-Ciocalteu's phenol reagent and by aluminium chloride method, respectively.

Results: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test. All the samples showed a dose dependent anti-inflammatory activity in HRBC membrane stabilization test. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined. Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.

Conclusion: Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

No MeSH data available.


Related in: MedlinePlus

Effect of CEE and CHF of C. cujete leaves and stem bark on hypotonic solution-induced hemolysis of erythrocyte membrane. Each value is expressed as mean ± SD (n = 3). CEE crude ethanol extract, CHF chloroform fraction
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Fig3: Effect of CEE and CHF of C. cujete leaves and stem bark on hypotonic solution-induced hemolysis of erythrocyte membrane. Each value is expressed as mean ± SD (n = 3). CEE crude ethanol extract, CHF chloroform fraction

Mentions: The results of anti-inflammatory activity determined by the human red blood cell membrane stabilization test were shown in Fig. 3. The CEE and CHF of leaves and bark showed a concentration dependent anti-inflammatory activity, and the protection percent increased with increase in the concentration of the samples. At concentration of 1.0 mg/ml, the CEE of leaves and bark produced 53.86 ± 6.37 and 61.85 ± 5.56 % inhibition of RBC hemolysis, respectively as compared with 75.80 ± 5.04 % produced by standard drug aspirin. Likewise, CHF of leaves and bark produced 48.74 ± 0.56 and 43.55 ± 6.20 % inhibition of RBC hemolysis, respectively. It is clear from the data that CEE of leaves and bark showed grater response than the CHF.Fig. 3


Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark.

Parvin MS, Das N, Jahan N, Akhter MA, Nahar L, Islam ME - BMC Res Notes (2015)

Effect of CEE and CHF of C. cujete leaves and stem bark on hypotonic solution-induced hemolysis of erythrocyte membrane. Each value is expressed as mean ± SD (n = 3). CEE crude ethanol extract, CHF chloroform fraction
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4559910&req=5

Fig3: Effect of CEE and CHF of C. cujete leaves and stem bark on hypotonic solution-induced hemolysis of erythrocyte membrane. Each value is expressed as mean ± SD (n = 3). CEE crude ethanol extract, CHF chloroform fraction
Mentions: The results of anti-inflammatory activity determined by the human red blood cell membrane stabilization test were shown in Fig. 3. The CEE and CHF of leaves and bark showed a concentration dependent anti-inflammatory activity, and the protection percent increased with increase in the concentration of the samples. At concentration of 1.0 mg/ml, the CEE of leaves and bark produced 53.86 ± 6.37 and 61.85 ± 5.56 % inhibition of RBC hemolysis, respectively as compared with 75.80 ± 5.04 % produced by standard drug aspirin. Likewise, CHF of leaves and bark produced 48.74 ± 0.56 and 43.55 ± 6.20 % inhibition of RBC hemolysis, respectively. It is clear from the data that CEE of leaves and bark showed grater response than the CHF.Fig. 3

Bottom Line: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test.Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. rumiraj2@yahoo.com.

ABSTRACT

Background: The various parts of Cresecentia cujete have some important biological activities. In folklore medicine leaves are used to treat hematomas, tumors and hypertension. Fruit decoction is used to treat diarrhea, stomachaches, cold, bronchitis, cough, asthma, and urethritis. The present study was designed to explore the anti-inflammatory and antibacterial potential of C. cujete leaves and stem bark. Anti-inflammatory activity was evaluated by in vitro human red blood cell (HRBC) membrane stabilization method and antibacterial activity by disc diffusion method.

Methods: In vitro anti-inflammatory activity was evaluated by human red blood cell (HRBC) membrane stabilization method while in vitro antibacterial activity was evaluated using cultures of Escherichia coli and Staphylococcus aureus by disc diffusion method. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined by Folin-Ciocalteu's phenol reagent and by aluminium chloride method, respectively.

Results: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test. All the samples showed a dose dependent anti-inflammatory activity in HRBC membrane stabilization test. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined. Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.

Conclusion: Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

No MeSH data available.


Related in: MedlinePlus