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Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark.

Parvin MS, Das N, Jahan N, Akhter MA, Nahar L, Islam ME - BMC Res Notes (2015)

Bottom Line: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test.Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. rumiraj2@yahoo.com.

ABSTRACT

Background: The various parts of Cresecentia cujete have some important biological activities. In folklore medicine leaves are used to treat hematomas, tumors and hypertension. Fruit decoction is used to treat diarrhea, stomachaches, cold, bronchitis, cough, asthma, and urethritis. The present study was designed to explore the anti-inflammatory and antibacterial potential of C. cujete leaves and stem bark. Anti-inflammatory activity was evaluated by in vitro human red blood cell (HRBC) membrane stabilization method and antibacterial activity by disc diffusion method.

Methods: In vitro anti-inflammatory activity was evaluated by human red blood cell (HRBC) membrane stabilization method while in vitro antibacterial activity was evaluated using cultures of Escherichia coli and Staphylococcus aureus by disc diffusion method. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined by Folin-Ciocalteu's phenol reagent and by aluminium chloride method, respectively.

Results: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test. All the samples showed a dose dependent anti-inflammatory activity in HRBC membrane stabilization test. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined. Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.

Conclusion: Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

No MeSH data available.


Related in: MedlinePlus

Total flavonoid content of leaves and bark. Values are represented as mg of QE/g of extract. Each value in the graph is represented as mean ± SD (n = 3)
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Fig2: Total flavonoid content of leaves and bark. Values are represented as mg of QE/g of extract. Each value in the graph is represented as mean ± SD (n = 3)

Mentions: TFC was expressed as mg of quercetin equivalents per gm of dry extract. Flavonoid contents varied widely among CEE and CHF of leaves and bark. CEE of leaves exhibited highest flavonoid contents (139.57 ± 3.75 mg of QE/gm of extract) followed by CHF of leaves, CHF and CEE of bark in the decreasing order (Fig. 2). TFC in the samples were determined with reference standard curve of quercetin (y = 0.0062x + 0.0039, R2 = 0.932).Fig. 2


Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark.

Parvin MS, Das N, Jahan N, Akhter MA, Nahar L, Islam ME - BMC Res Notes (2015)

Total flavonoid content of leaves and bark. Values are represented as mg of QE/g of extract. Each value in the graph is represented as mean ± SD (n = 3)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4559910&req=5

Fig2: Total flavonoid content of leaves and bark. Values are represented as mg of QE/g of extract. Each value in the graph is represented as mean ± SD (n = 3)
Mentions: TFC was expressed as mg of quercetin equivalents per gm of dry extract. Flavonoid contents varied widely among CEE and CHF of leaves and bark. CEE of leaves exhibited highest flavonoid contents (139.57 ± 3.75 mg of QE/gm of extract) followed by CHF of leaves, CHF and CEE of bark in the decreasing order (Fig. 2). TFC in the samples were determined with reference standard curve of quercetin (y = 0.0062x + 0.0039, R2 = 0.932).Fig. 2

Bottom Line: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test.Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. rumiraj2@yahoo.com.

ABSTRACT

Background: The various parts of Cresecentia cujete have some important biological activities. In folklore medicine leaves are used to treat hematomas, tumors and hypertension. Fruit decoction is used to treat diarrhea, stomachaches, cold, bronchitis, cough, asthma, and urethritis. The present study was designed to explore the anti-inflammatory and antibacterial potential of C. cujete leaves and stem bark. Anti-inflammatory activity was evaluated by in vitro human red blood cell (HRBC) membrane stabilization method and antibacterial activity by disc diffusion method.

Methods: In vitro anti-inflammatory activity was evaluated by human red blood cell (HRBC) membrane stabilization method while in vitro antibacterial activity was evaluated using cultures of Escherichia coli and Staphylococcus aureus by disc diffusion method. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined by Folin-Ciocalteu's phenol reagent and by aluminium chloride method, respectively.

Results: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test. All the samples showed a dose dependent anti-inflammatory activity in HRBC membrane stabilization test. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined. Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.

Conclusion: Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

No MeSH data available.


Related in: MedlinePlus