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Sex-related variations in bone microstructure of rabbits intramuscularly exposed to patulin.

Duranova H, Kovacova V, Babosova R, Omelka R, Adamkovicova M, Grosskopf B, Capcarova M, Martiniakova M - Acta Vet. Scand. (2015)

Bottom Line: A number of adverse health effects in humans and animals are associated with patulin intoxication.This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits.Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology and Anthropology, Constantine the Philosopher University, 949 74, Nitra, Slovakia. hduranova@ukf.sk.

ABSTRACT

Background: Patulin, a toxic mold metabolite, has been found as natural contaminant of processed fruits, most notably apples, apple juices and other apple-based products. A number of adverse health effects in humans and animals are associated with patulin intoxication. The current study was performed to analyse possible toxic effects of patulin on femoral bone microstructure in adult rabbits in detail. Fourteen clinically healthy four-month-old rabbits of both sexes (6 males and 8 females) were included in the study. Animals from the experimental groups (group E♂, n = 3; group E♀, n = 4) were injected intramuscularly with patulin at dose 10 μg/kg body weight two times a week for 28 days. The dose of patulin was estimated based on the maximum permitted level of patulin for apple products intended for infants and young children. Three males and four females without patulin administration served as controls (groups C♂ and C♀). Cortical bone thickness and qualitative and quantitative histological characteristics of compact bone tissue were investigated.

Results: Intramuscular applications of patulin significantly increased the thickness of cortical bone in both sexes of rabbits. In patulin-exposed males, an absence of primary vascular longitudinal bone tissue near the endosteal border was observed, which could be associated with intensive bone remodeling. Femoral diaphyses of females displayed a lower number of secondary osteons in the middle part of the substantia compacta, and occurrence of the osteons near the periosteum. This could indicate alterations in bone turnover. Histomorphometrical evaluations showed significantly increased sizes of the primary osteons' vascular canals (P < 0.05) in males exposed to patulin possibly due to mycotoxin-induced increased levels of testosterone.

Conclusions: This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits. Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.

No MeSH data available.


Related in: MedlinePlus

Microscopical structure of compact bone in rabbits from the group E♀. 1 Endosteal surface and the middle area of substantia compacta consisting of primary vascular longitudinal bone (arrows vascular canals of primary osteons). 2 Secondary osteons near the periosteum (arrows secondary osteons)
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Fig3: Microscopical structure of compact bone in rabbits from the group E♀. 1 Endosteal surface and the middle area of substantia compacta consisting of primary vascular longitudinal bone (arrows vascular canals of primary osteons). 2 Secondary osteons near the periosteum (arrows secondary osteons)

Mentions: In the femoral diaphysis of male rabbits exposed to patulin, subendosteal primary vascular longitudinal bone tissue was absent. This part of the bone consisted only of dense Haversian bone tissue. The periosteal surface was formed by primary vascular longitudinal bone which often also created the middle part of the compacta (Fig. 2). In female rabbits from group E♀ (Fig. 3), a lower number of secondary osteons was observed in the middle part of the bone (P < 0.05). From this part of the bone, secondary osteons were marginalized to periosteum suggesting alterations in bone turnover. In some individuals, the middle part of the substantia compacta consisted of primary vascular longitudinal bone, which in group C♀ rabbits created endosteal and periosteal surfaces.Fig. 2


Sex-related variations in bone microstructure of rabbits intramuscularly exposed to patulin.

Duranova H, Kovacova V, Babosova R, Omelka R, Adamkovicova M, Grosskopf B, Capcarova M, Martiniakova M - Acta Vet. Scand. (2015)

Microscopical structure of compact bone in rabbits from the group E♀. 1 Endosteal surface and the middle area of substantia compacta consisting of primary vascular longitudinal bone (arrows vascular canals of primary osteons). 2 Secondary osteons near the periosteum (arrows secondary osteons)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4559906&req=5

Fig3: Microscopical structure of compact bone in rabbits from the group E♀. 1 Endosteal surface and the middle area of substantia compacta consisting of primary vascular longitudinal bone (arrows vascular canals of primary osteons). 2 Secondary osteons near the periosteum (arrows secondary osteons)
Mentions: In the femoral diaphysis of male rabbits exposed to patulin, subendosteal primary vascular longitudinal bone tissue was absent. This part of the bone consisted only of dense Haversian bone tissue. The periosteal surface was formed by primary vascular longitudinal bone which often also created the middle part of the compacta (Fig. 2). In female rabbits from group E♀ (Fig. 3), a lower number of secondary osteons was observed in the middle part of the bone (P < 0.05). From this part of the bone, secondary osteons were marginalized to periosteum suggesting alterations in bone turnover. In some individuals, the middle part of the substantia compacta consisted of primary vascular longitudinal bone, which in group C♀ rabbits created endosteal and periosteal surfaces.Fig. 2

Bottom Line: A number of adverse health effects in humans and animals are associated with patulin intoxication.This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits.Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology and Anthropology, Constantine the Philosopher University, 949 74, Nitra, Slovakia. hduranova@ukf.sk.

ABSTRACT

Background: Patulin, a toxic mold metabolite, has been found as natural contaminant of processed fruits, most notably apples, apple juices and other apple-based products. A number of adverse health effects in humans and animals are associated with patulin intoxication. The current study was performed to analyse possible toxic effects of patulin on femoral bone microstructure in adult rabbits in detail. Fourteen clinically healthy four-month-old rabbits of both sexes (6 males and 8 females) were included in the study. Animals from the experimental groups (group E♂, n = 3; group E♀, n = 4) were injected intramuscularly with patulin at dose 10 μg/kg body weight two times a week for 28 days. The dose of patulin was estimated based on the maximum permitted level of patulin for apple products intended for infants and young children. Three males and four females without patulin administration served as controls (groups C♂ and C♀). Cortical bone thickness and qualitative and quantitative histological characteristics of compact bone tissue were investigated.

Results: Intramuscular applications of patulin significantly increased the thickness of cortical bone in both sexes of rabbits. In patulin-exposed males, an absence of primary vascular longitudinal bone tissue near the endosteal border was observed, which could be associated with intensive bone remodeling. Femoral diaphyses of females displayed a lower number of secondary osteons in the middle part of the substantia compacta, and occurrence of the osteons near the periosteum. This could indicate alterations in bone turnover. Histomorphometrical evaluations showed significantly increased sizes of the primary osteons' vascular canals (P < 0.05) in males exposed to patulin possibly due to mycotoxin-induced increased levels of testosterone.

Conclusions: This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits. Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.

No MeSH data available.


Related in: MedlinePlus