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Sex-related variations in bone microstructure of rabbits intramuscularly exposed to patulin.

Duranova H, Kovacova V, Babosova R, Omelka R, Adamkovicova M, Grosskopf B, Capcarova M, Martiniakova M - Acta Vet. Scand. (2015)

Bottom Line: A number of adverse health effects in humans and animals are associated with patulin intoxication.This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits.Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology and Anthropology, Constantine the Philosopher University, 949 74, Nitra, Slovakia. hduranova@ukf.sk.

ABSTRACT

Background: Patulin, a toxic mold metabolite, has been found as natural contaminant of processed fruits, most notably apples, apple juices and other apple-based products. A number of adverse health effects in humans and animals are associated with patulin intoxication. The current study was performed to analyse possible toxic effects of patulin on femoral bone microstructure in adult rabbits in detail. Fourteen clinically healthy four-month-old rabbits of both sexes (6 males and 8 females) were included in the study. Animals from the experimental groups (group E♂, n = 3; group E♀, n = 4) were injected intramuscularly with patulin at dose 10 μg/kg body weight two times a week for 28 days. The dose of patulin was estimated based on the maximum permitted level of patulin for apple products intended for infants and young children. Three males and four females without patulin administration served as controls (groups C♂ and C♀). Cortical bone thickness and qualitative and quantitative histological characteristics of compact bone tissue were investigated.

Results: Intramuscular applications of patulin significantly increased the thickness of cortical bone in both sexes of rabbits. In patulin-exposed males, an absence of primary vascular longitudinal bone tissue near the endosteal border was observed, which could be associated with intensive bone remodeling. Femoral diaphyses of females displayed a lower number of secondary osteons in the middle part of the substantia compacta, and occurrence of the osteons near the periosteum. This could indicate alterations in bone turnover. Histomorphometrical evaluations showed significantly increased sizes of the primary osteons' vascular canals (P < 0.05) in males exposed to patulin possibly due to mycotoxin-induced increased levels of testosterone.

Conclusions: This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits. Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.

No MeSH data available.


Related in: MedlinePlus

Microscopical structure of compact bone in rabbits from the control groups. 1 Primary vascular longitudinal bone tissue near endosteal and periosteal surfaces (arrows vascular canals of primary osteons). 2 Dense Haversian bone tissue creating the middle part of the substantia compacta (arrows secondary osteons)
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Fig1: Microscopical structure of compact bone in rabbits from the control groups. 1 Primary vascular longitudinal bone tissue near endosteal and periosteal surfaces (arrows vascular canals of primary osteons). 2 Dense Haversian bone tissue creating the middle part of the substantia compacta (arrows secondary osteons)

Mentions: The femoral diaphyses in male and female rabbits from the control groups displayed similar histological structure. Primary vascular longitudinal bone tissue, as a basic structural pattern of all bones, formed the inner layer surrounding the medullary cavity (endosteal border) and also periosteal surfaces of the substantia compacta. The tissue contained vascular canals which ran in a direction essentially parallel to the long axis of the bone. Near endosteal surfaces, primary vascular radial bone tissue composed of branching or non-branching vascular canals radiating from the marrow cavity, irregular and dense Haversian bone characterized by the occurrence of scattered secondary osteons and large numbers of secondary osteons, respectively, were also present. The middle part of the compact bone consisted of a layer of dense Haversian bone (Fig. 1).Fig. 1


Sex-related variations in bone microstructure of rabbits intramuscularly exposed to patulin.

Duranova H, Kovacova V, Babosova R, Omelka R, Adamkovicova M, Grosskopf B, Capcarova M, Martiniakova M - Acta Vet. Scand. (2015)

Microscopical structure of compact bone in rabbits from the control groups. 1 Primary vascular longitudinal bone tissue near endosteal and periosteal surfaces (arrows vascular canals of primary osteons). 2 Dense Haversian bone tissue creating the middle part of the substantia compacta (arrows secondary osteons)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4559906&req=5

Fig1: Microscopical structure of compact bone in rabbits from the control groups. 1 Primary vascular longitudinal bone tissue near endosteal and periosteal surfaces (arrows vascular canals of primary osteons). 2 Dense Haversian bone tissue creating the middle part of the substantia compacta (arrows secondary osteons)
Mentions: The femoral diaphyses in male and female rabbits from the control groups displayed similar histological structure. Primary vascular longitudinal bone tissue, as a basic structural pattern of all bones, formed the inner layer surrounding the medullary cavity (endosteal border) and also periosteal surfaces of the substantia compacta. The tissue contained vascular canals which ran in a direction essentially parallel to the long axis of the bone. Near endosteal surfaces, primary vascular radial bone tissue composed of branching or non-branching vascular canals radiating from the marrow cavity, irregular and dense Haversian bone characterized by the occurrence of scattered secondary osteons and large numbers of secondary osteons, respectively, were also present. The middle part of the compact bone consisted of a layer of dense Haversian bone (Fig. 1).Fig. 1

Bottom Line: A number of adverse health effects in humans and animals are associated with patulin intoxication.This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits.Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology and Anthropology, Constantine the Philosopher University, 949 74, Nitra, Slovakia. hduranova@ukf.sk.

ABSTRACT

Background: Patulin, a toxic mold metabolite, has been found as natural contaminant of processed fruits, most notably apples, apple juices and other apple-based products. A number of adverse health effects in humans and animals are associated with patulin intoxication. The current study was performed to analyse possible toxic effects of patulin on femoral bone microstructure in adult rabbits in detail. Fourteen clinically healthy four-month-old rabbits of both sexes (6 males and 8 females) were included in the study. Animals from the experimental groups (group E♂, n = 3; group E♀, n = 4) were injected intramuscularly with patulin at dose 10 μg/kg body weight two times a week for 28 days. The dose of patulin was estimated based on the maximum permitted level of patulin for apple products intended for infants and young children. Three males and four females without patulin administration served as controls (groups C♂ and C♀). Cortical bone thickness and qualitative and quantitative histological characteristics of compact bone tissue were investigated.

Results: Intramuscular applications of patulin significantly increased the thickness of cortical bone in both sexes of rabbits. In patulin-exposed males, an absence of primary vascular longitudinal bone tissue near the endosteal border was observed, which could be associated with intensive bone remodeling. Femoral diaphyses of females displayed a lower number of secondary osteons in the middle part of the substantia compacta, and occurrence of the osteons near the periosteum. This could indicate alterations in bone turnover. Histomorphometrical evaluations showed significantly increased sizes of the primary osteons' vascular canals (P < 0.05) in males exposed to patulin possibly due to mycotoxin-induced increased levels of testosterone.

Conclusions: This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits. Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.

No MeSH data available.


Related in: MedlinePlus