Replication kinetic of a representative outbreak-associ

Whole-genome Sequencing for Tracing the Transmission Link between Two ARD Outbreaks Caused by a Novel HAdV Serotype 7 Variant, China. Qiu S, Li P, Liu H, Wang Y, Liu N, Li C, Li S, Li M, Jiang Z, Sun H, Li Y, Xie J, Yang C, Wang J, Li H, Yi S, Wu Z, Jia L, Wang L, Hao R, Sun Y, Huang L, Ma H, Yuan Z, Song H - Sci Rep (2015) Bottom Line: WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates.Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate.Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions. View Article: PubMed Central - PubMed Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China. ABSTRACT From December 2012 to February 2013, two outbreaks of acute respiratory disease caused by HAdV-7 were reported in China. We investigated possible transmission links between these two seemingly unrelated outbreaks by integration of epidemiological and whole-genome sequencing (WGS) data. WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates. Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate. Epidemiological data supported that the first outbreak was caused by introduction of the novel HAdV-7 virus by an infected recruit upon arrival at the training base. Nosocomial transmission by close contacts was the most likely source leading to onset of the second HAdV-7 outbreak, establishing the apparent transmission link between the outbreaks. Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions. No MeSH data available. Related in: MedlinePlus	© Copyright Policy - open-access Related In: Results - Collection License Show All Figures getmorefigures.php?uid=PMC4559894&req=5 f5: Replication kinetic of a representative outbreak-associated isolate XY1 relative to non-outbreak-associated isolate CDC228.Since the outbreak isolates shared an indistinguishable genome content, a representative outbreak-associated isolate XY1 was used to evaluate the viral replication. The isolate XY1 was recovered from the first case (Index case A) from the Xiangyang outbreak, and the on-outbreak-associated isolate CDC228 was recovered from a sporadic ARD patient from Xiangyang City. Virus titers were determined in Hep-2 cells by measuring the 50% tissue culture infectious dose (TCID50). Mentions: The in vitro growth kinetics of viral replication were examined using isolate XY1 as representative of the outbreak-associated HAdV-7 viruses. Similar growth kinetics were observed with the non-outbreak associated isolate CDC228 between 0–12 h post-infection, while XY1 had a reproducibly higher viral titer than that of CDC228 between 12–48 h post-infection, indicating enhanced viral replication of outbreak isolates during this time period (Fig. 5).

Whole-genome Sequencing for Tracing the Transmission Link between Two ARD Outbreaks Caused by a Novel HAdV Serotype 7 Variant, China.

Qiu S, Li P, Liu H, Wang Y, Liu N, Li C, Li S, Li M, Jiang Z, Sun H, Li Y, Xie J, Yang C, Wang J, Li H, Yi S, Wu Z, Jia L, Wang L, Hao R, Sun Y, Huang L, Ma H, Yuan Z, Song H - Sci Rep (2015)

Bottom Line: WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates.Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate.Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.

ABSTRACT

From December 2012 to February 2013, two outbreaks of acute respiratory disease caused by HAdV-7 were reported in China. We investigated possible transmission links between these two seemingly unrelated outbreaks by integration of epidemiological and whole-genome sequencing (WGS) data. WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates. Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate. Epidemiological data supported that the first outbreak was caused by introduction of the novel HAdV-7 virus by an infected recruit upon arrival at the training base. Nosocomial transmission by close contacts was the most likely source leading to onset of the second HAdV-7 outbreak, establishing the apparent transmission link between the outbreaks. Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions.

No MeSH data available.

Related in: MedlinePlus

Replication kinetic of a representative outbreak-associated isolate XY1 relative to non-outbreak-associated isolate CDC228.Since the outbreak isolates shared an indistinguishable genome content, a representative outbreak-associated isolate XY1 was used to evaluate the viral replication. The isolate XY1 was recovered from the first case (Index case A) from the Xiangyang outbreak, and the on-outbreak-associated isolate CDC228 was recovered from a sporadic ARD patient from Xiangyang City. Virus titers were determined in Hep-2 cells by measuring the 50% tissue culture infectious dose (TCID50).

Related In: Results - Collection

License

Show All Figures

getmorefigures.php?uid=PMC4559894&req=5

f5: Replication kinetic of a representative outbreak-associated isolate XY1 relative to non-outbreak-associated isolate CDC228.Since the outbreak isolates shared an indistinguishable genome content, a representative outbreak-associated isolate XY1 was used to evaluate the viral replication. The isolate XY1 was recovered from the first case (Index case A) from the Xiangyang outbreak, and the on-outbreak-associated isolate CDC228 was recovered from a sporadic ARD patient from Xiangyang City. Virus titers were determined in Hep-2 cells by measuring the 50% tissue culture infectious dose (TCID50).

Mentions: The in vitro growth kinetics of viral replication were examined using isolate XY1 as representative of the outbreak-associated HAdV-7 viruses. Similar growth kinetics were observed with the non-outbreak associated isolate CDC228 between 0–12 h post-infection, while XY1 had a reproducibly higher viral titer than that of CDC228 between 12–48 h post-infection, indicating enhanced viral replication of outbreak isolates during this time period (Fig. 5).