Whole-genome Sequencing for Tracing the Transmission Link between Two ARD Outbreaks Caused by a Novel HAdV Serotype 7 Variant, China.
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WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates.Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate.Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions.
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PubMed Central - PubMed
Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
ABSTRACT
From December 2012 to February 2013, two outbreaks of acute respiratory disease caused by HAdV-7 were reported in China. We investigated possible transmission links between these two seemingly unrelated outbreaks by integration of epidemiological and whole-genome sequencing (WGS) data. WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates. Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate. Epidemiological data supported that the first outbreak was caused by introduction of the novel HAdV-7 virus by an infected recruit upon arrival at the training base. Nosocomial transmission by close contacts was the most likely source leading to onset of the second HAdV-7 outbreak, establishing the apparent transmission link between the outbreaks. Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions. No MeSH data available. Related in: MedlinePlus |
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f5: Replication kinetic of a representative outbreak-associated isolate XY1 relative to non-outbreak-associated isolate CDC228.Since the outbreak isolates shared an indistinguishable genome content, a representative outbreak-associated isolate XY1 was used to evaluate the viral replication. The isolate XY1 was recovered from the first case (Index case A) from the Xiangyang outbreak, and the on-outbreak-associated isolate CDC228 was recovered from a sporadic ARD patient from Xiangyang City. Virus titers were determined in Hep-2 cells by measuring the 50% tissue culture infectious dose (TCID50). Mentions: The in vitro growth kinetics of viral replication were examined using isolate XY1 as representative of the outbreak-associated HAdV-7 viruses. Similar growth kinetics were observed with the non-outbreak associated isolate CDC228 between 0–12 h post-infection, while XY1 had a reproducibly higher viral titer than that of CDC228 between 12–48 h post-infection, indicating enhanced viral replication of outbreak isolates during this time period (Fig. 5). |
View Article: PubMed Central - PubMed
Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
No MeSH data available.