Cases of confirmed and probable HAdV-7 infections durin

Whole-genome Sequencing for Tracing the Transmission Link between Two ARD Outbreaks Caused by a Novel HAdV Serotype 7 Variant, China. Qiu S, Li P, Liu H, Wang Y, Liu N, Li C, Li S, Li M, Jiang Z, Sun H, Li Y, Xie J, Yang C, Wang J, Li H, Yi S, Wu Z, Jia L, Wang L, Hao R, Sun Y, Huang L, Ma H, Yuan Z, Song H - Sci Rep (2015) Bottom Line: WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates.Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate.Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions. View Article: PubMed Central - PubMed Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China. ABSTRACT From December 2012 to February 2013, two outbreaks of acute respiratory disease caused by HAdV-7 were reported in China. We investigated possible transmission links between these two seemingly unrelated outbreaks by integration of epidemiological and whole-genome sequencing (WGS) data. WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates. Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate. Epidemiological data supported that the first outbreak was caused by introduction of the novel HAdV-7 virus by an infected recruit upon arrival at the training base. Nosocomial transmission by close contacts was the most likely source leading to onset of the second HAdV-7 outbreak, establishing the apparent transmission link between the outbreaks. Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions. No MeSH data available. Related in: MedlinePlus	© Copyright Policy - open-access Related In: Results - Collection License Show All Figures getmorefigures.php?uid=PMC4559894&req=5 f1: Cases of confirmed and probable HAdV-7 infections during the two outbreaks.A total of 946 persons were affected during the outbreaks. We collected 328 and 80 nasopharyngeal specimens from the two outbreak patients, respectively, and 323 HAdV-7 isolates were identified by PCR assays and viral culture. Mentions: Base A (Xiangyang) houses 1290 recruits, of whom 858 (66.5%) fell ill with ARD from December 10, 2012 to January 28, 2013 (Fig. 1). Among the 858 cases, 321 (37.4%) were hospitalized, 108 (12.6%) developed acute pneumonia and 37 were considered severe cases. Base B (Jingmen) houses 865 recruits; 88 (10.2%) were diagnosed with ARD from February 11 to 27, 2013. Among the 88 cases, 42 (47.7%) were hospitalized, 16 (18.2%) with pneumonia and 4 severe cases were recorded. Under normal circumstances, these training facilities are not directly connected; this is primarily due to the geographic separation (Figure S1) and self-contained management systems. The initial hypothesis was that these outbreaks were unrelated.

Whole-genome Sequencing for Tracing the Transmission Link between Two ARD Outbreaks Caused by a Novel HAdV Serotype 7 Variant, China.

Qiu S, Li P, Liu H, Wang Y, Liu N, Li C, Li S, Li M, Jiang Z, Sun H, Li Y, Xie J, Yang C, Wang J, Li H, Yi S, Wu Z, Jia L, Wang L, Hao R, Sun Y, Huang L, Ma H, Yuan Z, Song H - Sci Rep (2015)

Bottom Line: WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates.Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate.Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.

ABSTRACT

From December 2012 to February 2013, two outbreaks of acute respiratory disease caused by HAdV-7 were reported in China. We investigated possible transmission links between these two seemingly unrelated outbreaks by integration of epidemiological and whole-genome sequencing (WGS) data. WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates. Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate. Epidemiological data supported that the first outbreak was caused by introduction of the novel HAdV-7 virus by an infected recruit upon arrival at the training base. Nosocomial transmission by close contacts was the most likely source leading to onset of the second HAdV-7 outbreak, establishing the apparent transmission link between the outbreaks. Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions.

No MeSH data available.

Related in: MedlinePlus

Cases of confirmed and probable HAdV-7 infections during the two outbreaks.A total of 946 persons were affected during the outbreaks. We collected 328 and 80 nasopharyngeal specimens from the two outbreak patients, respectively, and 323 HAdV-7 isolates were identified by PCR assays and viral culture.

Related In: Results - Collection

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f1: Cases of confirmed and probable HAdV-7 infections during the two outbreaks.A total of 946 persons were affected during the outbreaks. We collected 328 and 80 nasopharyngeal specimens from the two outbreak patients, respectively, and 323 HAdV-7 isolates were identified by PCR assays and viral culture.

Mentions: Base A (Xiangyang) houses 1290 recruits, of whom 858 (66.5%) fell ill with ARD from December 10, 2012 to January 28, 2013 (Fig. 1). Among the 858 cases, 321 (37.4%) were hospitalized, 108 (12.6%) developed acute pneumonia and 37 were considered severe cases. Base B (Jingmen) houses 865 recruits; 88 (10.2%) were diagnosed with ARD from February 11 to 27, 2013. Among the 88 cases, 42 (47.7%) were hospitalized, 16 (18.2%) with pneumonia and 4 severe cases were recorded. Under normal circumstances, these training facilities are not directly connected; this is primarily due to the geographic separation (Figure S1) and self-contained management systems. The initial hypothesis was that these outbreaks were unrelated.