Whole-genome Sequencing for Tracing the Transmission Link between Two ARD Outbreaks Caused by a Novel HAdV Serotype 7 Variant, China.
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WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates.Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate.Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions.
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PubMed Central - PubMed
Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
ABSTRACT
From December 2012 to February 2013, two outbreaks of acute respiratory disease caused by HAdV-7 were reported in China. We investigated possible transmission links between these two seemingly unrelated outbreaks by integration of epidemiological and whole-genome sequencing (WGS) data. WGS analyses showed that the HAdV-7 isolates from the two outbreaks were genetically indistinguishable; however, a 12 bp deletion in the virus-associated RNA gene distinguished the outbreak isolates from other HAdV-7 isolates. Outbreak HAdV-7 isolates demonstrated increased viral replication compared to non-outbreak associated HAdV-7 isolate. Epidemiological data supported that the first outbreak was caused by introduction of the novel HAdV-7 virus by an infected recruit upon arrival at the training base. Nosocomial transmission by close contacts was the most likely source leading to onset of the second HAdV-7 outbreak, establishing the apparent transmission link between the outbreaks. Our findings imply that in-hospital contact investigations should be encouraged to reduce or interrupt further spread of infectious agents when treating outbreak cases, and WGS can provide useful information guiding infection-control interventions. No MeSH data available. Related in: MedlinePlus |
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f1: Cases of confirmed and probable HAdV-7 infections during the two outbreaks.A total of 946 persons were affected during the outbreaks. We collected 328 and 80 nasopharyngeal specimens from the two outbreak patients, respectively, and 323 HAdV-7 isolates were identified by PCR assays and viral culture. Mentions: Base A (Xiangyang) houses 1290 recruits, of whom 858 (66.5%) fell ill with ARD from December 10, 2012 to January 28, 2013 (Fig. 1). Among the 858 cases, 321 (37.4%) were hospitalized, 108 (12.6%) developed acute pneumonia and 37 were considered severe cases. Base B (Jingmen) houses 865 recruits; 88 (10.2%) were diagnosed with ARD from February 11 to 27, 2013. Among the 88 cases, 42 (47.7%) were hospitalized, 16 (18.2%) with pneumonia and 4 severe cases were recorded. Under normal circumstances, these training facilities are not directly connected; this is primarily due to the geographic separation (Figure S1) and self-contained management systems. The initial hypothesis was that these outbreaks were unrelated. |
View Article: PubMed Central - PubMed
Affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
No MeSH data available.