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The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.

Kuroda K, Kabata T, Hayashi K, Maeda T, Kajino Y, Iwai S, Fujita K, Hasegawa K, Inoue D, Sugimoto N, Tsuchiya H - BMC Musculoskelet Disord (2015)

Bottom Line: The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks.TNF-alpha-induced apoptotic stimulation was similar between the two groups.Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kurokazu0108@yahoo.co.jp.

ABSTRACT

Background: This study aimed to determine whether intra-articularly injected adipose-derived stem cells (ADSCs) inhibited articular cartilage degeneration during osteoarthritis (OA) development in a rabbit anterior cruciate ligament transection (ACLT) model. The paracrine effects of ADSCs on chondrocytes were investigated using a co-culture system.

Methods: ACLT was performed on both knee joints of 12 rabbits. ADSCs were isolated from the subcutaneous adipose tissue. ADSCs with hyaluronic acid were intra-articularly injected into the left knee, and hyaluronic acid was injected into the right knee. The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks. In addition, cell viability was determined using co-culture system of ADSCs and chondrocytes.

Results: Macroscopically, osteoarthritis progression was milder in the ADSC-treated knees than in the control knees 8 weeks after ACLT. Histologically, control knees showed obvious erosions in both the medial and lateral condyles at 8 weeks, while cartilage was predominantly retained in the ADSC-treated knees. At 12 weeks, the ADSC-treated knees showed a slight suppression of cartilage degeneration, unlike the control knees. Immunohistochemically, MMP-13 expression was less in the ADSC-treated cartilage than in the control knees. The cell viability of chondrocytes co-cultured with ADSCs was higher than that of chondrocytes cultured alone. TNF-alpha-induced apoptotic stimulation was similar between the two groups.

Conclusions: Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

No MeSH data available.


Related in: MedlinePlus

MMP-13 levels in medium of co-culture of ADSCs and chondrocytes with TNF-α and chondrocyte cultured alone with TNF-α. MMP-13 was measured by ELIZA
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Fig7: MMP-13 levels in medium of co-culture of ADSCs and chondrocytes with TNF-α and chondrocyte cultured alone with TNF-α. MMP-13 was measured by ELIZA

Mentions: To confirm the paracrine effect of ADSCs on MMP-13 expression, the concentration of MMP-13 in the co-culture and control groups was evaluated by ELIZA. At 72 h after TNF-α stimulation, the concentration of MMP-13 in the medium of the co-culture group was lower than that in the medium of the chondrocyte alone group, as shown in Fig. 7. This indicates that the expression of MMP-13 was reduced by the paracrine effect of the ADSCs.Fig. 7


The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.

Kuroda K, Kabata T, Hayashi K, Maeda T, Kajino Y, Iwai S, Fujita K, Hasegawa K, Inoue D, Sugimoto N, Tsuchiya H - BMC Musculoskelet Disord (2015)

MMP-13 levels in medium of co-culture of ADSCs and chondrocytes with TNF-α and chondrocyte cultured alone with TNF-α. MMP-13 was measured by ELIZA
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4559871&req=5

Fig7: MMP-13 levels in medium of co-culture of ADSCs and chondrocytes with TNF-α and chondrocyte cultured alone with TNF-α. MMP-13 was measured by ELIZA
Mentions: To confirm the paracrine effect of ADSCs on MMP-13 expression, the concentration of MMP-13 in the co-culture and control groups was evaluated by ELIZA. At 72 h after TNF-α stimulation, the concentration of MMP-13 in the medium of the co-culture group was lower than that in the medium of the chondrocyte alone group, as shown in Fig. 7. This indicates that the expression of MMP-13 was reduced by the paracrine effect of the ADSCs.Fig. 7

Bottom Line: The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks.TNF-alpha-induced apoptotic stimulation was similar between the two groups.Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kurokazu0108@yahoo.co.jp.

ABSTRACT

Background: This study aimed to determine whether intra-articularly injected adipose-derived stem cells (ADSCs) inhibited articular cartilage degeneration during osteoarthritis (OA) development in a rabbit anterior cruciate ligament transection (ACLT) model. The paracrine effects of ADSCs on chondrocytes were investigated using a co-culture system.

Methods: ACLT was performed on both knee joints of 12 rabbits. ADSCs were isolated from the subcutaneous adipose tissue. ADSCs with hyaluronic acid were intra-articularly injected into the left knee, and hyaluronic acid was injected into the right knee. The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks. In addition, cell viability was determined using co-culture system of ADSCs and chondrocytes.

Results: Macroscopically, osteoarthritis progression was milder in the ADSC-treated knees than in the control knees 8 weeks after ACLT. Histologically, control knees showed obvious erosions in both the medial and lateral condyles at 8 weeks, while cartilage was predominantly retained in the ADSC-treated knees. At 12 weeks, the ADSC-treated knees showed a slight suppression of cartilage degeneration, unlike the control knees. Immunohistochemically, MMP-13 expression was less in the ADSC-treated cartilage than in the control knees. The cell viability of chondrocytes co-cultured with ADSCs was higher than that of chondrocytes cultured alone. TNF-alpha-induced apoptotic stimulation was similar between the two groups.

Conclusions: Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

No MeSH data available.


Related in: MedlinePlus