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The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.

Kuroda K, Kabata T, Hayashi K, Maeda T, Kajino Y, Iwai S, Fujita K, Hasegawa K, Inoue D, Sugimoto N, Tsuchiya H - BMC Musculoskelet Disord (2015)

Bottom Line: The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks.TNF-alpha-induced apoptotic stimulation was similar between the two groups.Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kurokazu0108@yahoo.co.jp.

ABSTRACT

Background: This study aimed to determine whether intra-articularly injected adipose-derived stem cells (ADSCs) inhibited articular cartilage degeneration during osteoarthritis (OA) development in a rabbit anterior cruciate ligament transection (ACLT) model. The paracrine effects of ADSCs on chondrocytes were investigated using a co-culture system.

Methods: ACLT was performed on both knee joints of 12 rabbits. ADSCs were isolated from the subcutaneous adipose tissue. ADSCs with hyaluronic acid were intra-articularly injected into the left knee, and hyaluronic acid was injected into the right knee. The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks. In addition, cell viability was determined using co-culture system of ADSCs and chondrocytes.

Results: Macroscopically, osteoarthritis progression was milder in the ADSC-treated knees than in the control knees 8 weeks after ACLT. Histologically, control knees showed obvious erosions in both the medial and lateral condyles at 8 weeks, while cartilage was predominantly retained in the ADSC-treated knees. At 12 weeks, the ADSC-treated knees showed a slight suppression of cartilage degeneration, unlike the control knees. Immunohistochemically, MMP-13 expression was less in the ADSC-treated cartilage than in the control knees. The cell viability of chondrocytes co-cultured with ADSCs was higher than that of chondrocytes cultured alone. TNF-alpha-induced apoptotic stimulation was similar between the two groups.

Conclusions: Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

No MeSH data available.


Related in: MedlinePlus

DiI labeling at 8 weeks (a) and 12 weeks (b) after ACLT; After intra-articular injection of ADSCs labeled with DiI dye, frozen sections were prepared and then stained with hematoxylin and eosin. DiI positive cells survived and homed to the subintimal layers of the synovium (S) and ligament (L), not in the cartilage at 8 weeks after ACLT
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Fig5: DiI labeling at 8 weeks (a) and 12 weeks (b) after ACLT; After intra-articular injection of ADSCs labeled with DiI dye, frozen sections were prepared and then stained with hematoxylin and eosin. DiI positive cells survived and homed to the subintimal layers of the synovium (S) and ligament (L), not in the cartilage at 8 weeks after ACLT

Mentions: DiI positive areas are shown in Fig. 5a and b. Figure 5a reveals that intra-articular injected cells survived and homed to the subintimal layers of the synovium and ligament, but not the cartilage, 8 weeks after ACLT. On the other hand, at 12 weeks after ACLT, no DiI positive cells were observed (Fig. 5b).Fig. 5


The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.

Kuroda K, Kabata T, Hayashi K, Maeda T, Kajino Y, Iwai S, Fujita K, Hasegawa K, Inoue D, Sugimoto N, Tsuchiya H - BMC Musculoskelet Disord (2015)

DiI labeling at 8 weeks (a) and 12 weeks (b) after ACLT; After intra-articular injection of ADSCs labeled with DiI dye, frozen sections were prepared and then stained with hematoxylin and eosin. DiI positive cells survived and homed to the subintimal layers of the synovium (S) and ligament (L), not in the cartilage at 8 weeks after ACLT
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4559871&req=5

Fig5: DiI labeling at 8 weeks (a) and 12 weeks (b) after ACLT; After intra-articular injection of ADSCs labeled with DiI dye, frozen sections were prepared and then stained with hematoxylin and eosin. DiI positive cells survived and homed to the subintimal layers of the synovium (S) and ligament (L), not in the cartilage at 8 weeks after ACLT
Mentions: DiI positive areas are shown in Fig. 5a and b. Figure 5a reveals that intra-articular injected cells survived and homed to the subintimal layers of the synovium and ligament, but not the cartilage, 8 weeks after ACLT. On the other hand, at 12 weeks after ACLT, no DiI positive cells were observed (Fig. 5b).Fig. 5

Bottom Line: The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks.TNF-alpha-induced apoptotic stimulation was similar between the two groups.Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kurokazu0108@yahoo.co.jp.

ABSTRACT

Background: This study aimed to determine whether intra-articularly injected adipose-derived stem cells (ADSCs) inhibited articular cartilage degeneration during osteoarthritis (OA) development in a rabbit anterior cruciate ligament transection (ACLT) model. The paracrine effects of ADSCs on chondrocytes were investigated using a co-culture system.

Methods: ACLT was performed on both knee joints of 12 rabbits. ADSCs were isolated from the subcutaneous adipose tissue. ADSCs with hyaluronic acid were intra-articularly injected into the left knee, and hyaluronic acid was injected into the right knee. The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks. In addition, cell viability was determined using co-culture system of ADSCs and chondrocytes.

Results: Macroscopically, osteoarthritis progression was milder in the ADSC-treated knees than in the control knees 8 weeks after ACLT. Histologically, control knees showed obvious erosions in both the medial and lateral condyles at 8 weeks, while cartilage was predominantly retained in the ADSC-treated knees. At 12 weeks, the ADSC-treated knees showed a slight suppression of cartilage degeneration, unlike the control knees. Immunohistochemically, MMP-13 expression was less in the ADSC-treated cartilage than in the control knees. The cell viability of chondrocytes co-cultured with ADSCs was higher than that of chondrocytes cultured alone. TNF-alpha-induced apoptotic stimulation was similar between the two groups.

Conclusions: Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

No MeSH data available.


Related in: MedlinePlus