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The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.

Kuroda K, Kabata T, Hayashi K, Maeda T, Kajino Y, Iwai S, Fujita K, Hasegawa K, Inoue D, Sugimoto N, Tsuchiya H - BMC Musculoskelet Disord (2015)

Bottom Line: The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks.TNF-alpha-induced apoptotic stimulation was similar between the two groups.Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kurokazu0108@yahoo.co.jp.

ABSTRACT

Background: This study aimed to determine whether intra-articularly injected adipose-derived stem cells (ADSCs) inhibited articular cartilage degeneration during osteoarthritis (OA) development in a rabbit anterior cruciate ligament transection (ACLT) model. The paracrine effects of ADSCs on chondrocytes were investigated using a co-culture system.

Methods: ACLT was performed on both knee joints of 12 rabbits. ADSCs were isolated from the subcutaneous adipose tissue. ADSCs with hyaluronic acid were intra-articularly injected into the left knee, and hyaluronic acid was injected into the right knee. The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks. In addition, cell viability was determined using co-culture system of ADSCs and chondrocytes.

Results: Macroscopically, osteoarthritis progression was milder in the ADSC-treated knees than in the control knees 8 weeks after ACLT. Histologically, control knees showed obvious erosions in both the medial and lateral condyles at 8 weeks, while cartilage was predominantly retained in the ADSC-treated knees. At 12 weeks, the ADSC-treated knees showed a slight suppression of cartilage degeneration, unlike the control knees. Immunohistochemically, MMP-13 expression was less in the ADSC-treated cartilage than in the control knees. The cell viability of chondrocytes co-cultured with ADSCs was higher than that of chondrocytes cultured alone. TNF-alpha-induced apoptotic stimulation was similar between the two groups.

Conclusions: Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

No MeSH data available.


Related in: MedlinePlus

Macroscopic analyses of the femoral condyles. a Representative specimen 8 weeks after ACLT. To remove individual viability, both sides of the knees of the same individuals are shown. The surface of the cartilage was stained with India ink to identify any fibrillation and erosion. Laterality is shown as medial (M) and lateral (L). b Macroscopic osteoarthritis score 8 weeks after ACLT (n = 6). c Macroscopic osteoarthritis score 12 weeks after ACLT (n = 6)
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Fig2: Macroscopic analyses of the femoral condyles. a Representative specimen 8 weeks after ACLT. To remove individual viability, both sides of the knees of the same individuals are shown. The surface of the cartilage was stained with India ink to identify any fibrillation and erosion. Laterality is shown as medial (M) and lateral (L). b Macroscopic osteoarthritis score 8 weeks after ACLT (n = 6). c Macroscopic osteoarthritis score 12 weeks after ACLT (n = 6)

Mentions: Figure 2a shows an example of a typical control and ADSC-treated condyle, 8 weeks after ACLT, stained with india ink. In the control knees, erosions in both the medial and lateral condyles can be seen. On the other hand, in the ADSC-treated knees, no erosion was observed; there were no macroscopically identifiable cartilage lesions in the medial condyle, and little fibrillation in the lateral condyle.Fig. 2


The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.

Kuroda K, Kabata T, Hayashi K, Maeda T, Kajino Y, Iwai S, Fujita K, Hasegawa K, Inoue D, Sugimoto N, Tsuchiya H - BMC Musculoskelet Disord (2015)

Macroscopic analyses of the femoral condyles. a Representative specimen 8 weeks after ACLT. To remove individual viability, both sides of the knees of the same individuals are shown. The surface of the cartilage was stained with India ink to identify any fibrillation and erosion. Laterality is shown as medial (M) and lateral (L). b Macroscopic osteoarthritis score 8 weeks after ACLT (n = 6). c Macroscopic osteoarthritis score 12 weeks after ACLT (n = 6)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4559871&req=5

Fig2: Macroscopic analyses of the femoral condyles. a Representative specimen 8 weeks after ACLT. To remove individual viability, both sides of the knees of the same individuals are shown. The surface of the cartilage was stained with India ink to identify any fibrillation and erosion. Laterality is shown as medial (M) and lateral (L). b Macroscopic osteoarthritis score 8 weeks after ACLT (n = 6). c Macroscopic osteoarthritis score 12 weeks after ACLT (n = 6)
Mentions: Figure 2a shows an example of a typical control and ADSC-treated condyle, 8 weeks after ACLT, stained with india ink. In the control knees, erosions in both the medial and lateral condyles can be seen. On the other hand, in the ADSC-treated knees, no erosion was observed; there were no macroscopically identifiable cartilage lesions in the medial condyle, and little fibrillation in the lateral condyle.Fig. 2

Bottom Line: The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks.TNF-alpha-induced apoptotic stimulation was similar between the two groups.Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kurokazu0108@yahoo.co.jp.

ABSTRACT

Background: This study aimed to determine whether intra-articularly injected adipose-derived stem cells (ADSCs) inhibited articular cartilage degeneration during osteoarthritis (OA) development in a rabbit anterior cruciate ligament transection (ACLT) model. The paracrine effects of ADSCs on chondrocytes were investigated using a co-culture system.

Methods: ACLT was performed on both knee joints of 12 rabbits. ADSCs were isolated from the subcutaneous adipose tissue. ADSCs with hyaluronic acid were intra-articularly injected into the left knee, and hyaluronic acid was injected into the right knee. The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks. In addition, cell viability was determined using co-culture system of ADSCs and chondrocytes.

Results: Macroscopically, osteoarthritis progression was milder in the ADSC-treated knees than in the control knees 8 weeks after ACLT. Histologically, control knees showed obvious erosions in both the medial and lateral condyles at 8 weeks, while cartilage was predominantly retained in the ADSC-treated knees. At 12 weeks, the ADSC-treated knees showed a slight suppression of cartilage degeneration, unlike the control knees. Immunohistochemically, MMP-13 expression was less in the ADSC-treated cartilage than in the control knees. The cell viability of chondrocytes co-cultured with ADSCs was higher than that of chondrocytes cultured alone. TNF-alpha-induced apoptotic stimulation was similar between the two groups.

Conclusions: Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.

No MeSH data available.


Related in: MedlinePlus