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Differential expression of metabotropic glutamate and GABA receptors at neocortical glutamatergic and GABAergic axon terminals.

Bragina L, Bonifacino T, Bassi S, Milanese M, Bonanno G, Conti F - Front Cell Neurosci (2015)

Bottom Line: VGLUT1-positive puncta expressed mGluR1α (∼5%), mGluR5 (∼6%), mGluR2/3 (∼22%), mGluR7 (∼17%), and GABAB1 (∼40%); VGLUT2-positive terminals expressed mGluR1α (∼10%), mGluR5 (∼11%), mGluR2/3 (∼20%), mGluR7 (∼28%), and GABAB1 (∼25%); whereas VGAT-positive puncta expressed mGluR1α (∼27%), mGluR5 (∼24%), mGluR2/3 (∼38%), mGluR7 (∼31%), and GABAB1 (∼19%).Control experiments ruled out the possibility that postsynaptic mGluRs and GABAB1 might have significantly biased our results.Overall, these findings indicate that mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 expression differ significantly between glutamatergic and GABAergic axon terminals, and that the robust expression of heteroreceptors may contribute to the homeostatic regulation of the balance between excitation and inhibition.

View Article: PubMed Central - PubMed

Affiliation: Section of Neuroscience and Cell Biology, Department of Experimental and Clinical Medicine, Università Politecnica delle Marche Ancona, Italy ; Center for Neurobiology of Aging, Istituto Nazionale di Riposo e Cura per Anziani - Istituto di Ricovero e Cura a Carattere Scientifico Ancona, Italy.

ABSTRACT
Metabotropic glutamate (Glu) receptors (mGluRs) and GABAB receptors are highly expressed at presynaptic sites. To verify the possibility that the two classes of metabotropic receptors contribute to axon terminals heterogeneity, we studied the localization of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 in VGLUT1-, VGLUT2-, and VGAT- positive terminals in the cerebral cortex of adult rats. VGLUT1-positive puncta expressed mGluR1α (∼5%), mGluR5 (∼6%), mGluR2/3 (∼22%), mGluR7 (∼17%), and GABAB1 (∼40%); VGLUT2-positive terminals expressed mGluR1α (∼10%), mGluR5 (∼11%), mGluR2/3 (∼20%), mGluR7 (∼28%), and GABAB1 (∼25%); whereas VGAT-positive puncta expressed mGluR1α (∼27%), mGluR5 (∼24%), mGluR2/3 (∼38%), mGluR7 (∼31%), and GABAB1 (∼19%). Control experiments ruled out the possibility that postsynaptic mGluRs and GABAB1 might have significantly biased our results. We also performed functional assays in synaptosomal preparations, and showed that all agonists modify Glu and GABA levels, which return to baseline upon exposure to antagonists. Overall, these findings indicate that mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 expression differ significantly between glutamatergic and GABAergic axon terminals, and that the robust expression of heteroreceptors may contribute to the homeostatic regulation of the balance between excitation and inhibition.

No MeSH data available.


Related in: MedlinePlus

Expression of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 in VGLUT1+, VGLUT2+, and VGAT+ axon terminals in cerebral cortex. Values are mean ± SEM; ∗∗p < 0.01, ∗∗∗p < 0.001.
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Figure 3: Expression of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 in VGLUT1+, VGLUT2+, and VGAT+ axon terminals in cerebral cortex. Values are mean ± SEM; ∗∗p < 0.01, ∗∗∗p < 0.001.

Mentions: Expression of mGluRs and GABAB1 in VGLUT1+ cortical axon terminals was studied in 25 sections from eight rats (Figures 2 and 3).


Differential expression of metabotropic glutamate and GABA receptors at neocortical glutamatergic and GABAergic axon terminals.

Bragina L, Bonifacino T, Bassi S, Milanese M, Bonanno G, Conti F - Front Cell Neurosci (2015)

Expression of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 in VGLUT1+, VGLUT2+, and VGAT+ axon terminals in cerebral cortex. Values are mean ± SEM; ∗∗p < 0.01, ∗∗∗p < 0.001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4559644&req=5

Figure 3: Expression of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 in VGLUT1+, VGLUT2+, and VGAT+ axon terminals in cerebral cortex. Values are mean ± SEM; ∗∗p < 0.01, ∗∗∗p < 0.001.
Mentions: Expression of mGluRs and GABAB1 in VGLUT1+ cortical axon terminals was studied in 25 sections from eight rats (Figures 2 and 3).

Bottom Line: VGLUT1-positive puncta expressed mGluR1α (∼5%), mGluR5 (∼6%), mGluR2/3 (∼22%), mGluR7 (∼17%), and GABAB1 (∼40%); VGLUT2-positive terminals expressed mGluR1α (∼10%), mGluR5 (∼11%), mGluR2/3 (∼20%), mGluR7 (∼28%), and GABAB1 (∼25%); whereas VGAT-positive puncta expressed mGluR1α (∼27%), mGluR5 (∼24%), mGluR2/3 (∼38%), mGluR7 (∼31%), and GABAB1 (∼19%).Control experiments ruled out the possibility that postsynaptic mGluRs and GABAB1 might have significantly biased our results.Overall, these findings indicate that mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 expression differ significantly between glutamatergic and GABAergic axon terminals, and that the robust expression of heteroreceptors may contribute to the homeostatic regulation of the balance between excitation and inhibition.

View Article: PubMed Central - PubMed

Affiliation: Section of Neuroscience and Cell Biology, Department of Experimental and Clinical Medicine, Università Politecnica delle Marche Ancona, Italy ; Center for Neurobiology of Aging, Istituto Nazionale di Riposo e Cura per Anziani - Istituto di Ricovero e Cura a Carattere Scientifico Ancona, Italy.

ABSTRACT
Metabotropic glutamate (Glu) receptors (mGluRs) and GABAB receptors are highly expressed at presynaptic sites. To verify the possibility that the two classes of metabotropic receptors contribute to axon terminals heterogeneity, we studied the localization of mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 in VGLUT1-, VGLUT2-, and VGAT- positive terminals in the cerebral cortex of adult rats. VGLUT1-positive puncta expressed mGluR1α (∼5%), mGluR5 (∼6%), mGluR2/3 (∼22%), mGluR7 (∼17%), and GABAB1 (∼40%); VGLUT2-positive terminals expressed mGluR1α (∼10%), mGluR5 (∼11%), mGluR2/3 (∼20%), mGluR7 (∼28%), and GABAB1 (∼25%); whereas VGAT-positive puncta expressed mGluR1α (∼27%), mGluR5 (∼24%), mGluR2/3 (∼38%), mGluR7 (∼31%), and GABAB1 (∼19%). Control experiments ruled out the possibility that postsynaptic mGluRs and GABAB1 might have significantly biased our results. We also performed functional assays in synaptosomal preparations, and showed that all agonists modify Glu and GABA levels, which return to baseline upon exposure to antagonists. Overall, these findings indicate that mGluR1α, mGluR5, mGluR2/3, mGluR7, and GABAB1 expression differ significantly between glutamatergic and GABAergic axon terminals, and that the robust expression of heteroreceptors may contribute to the homeostatic regulation of the balance between excitation and inhibition.

No MeSH data available.


Related in: MedlinePlus