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Epidermal Growth Factor Receptor Expression in Triple Negative and Nontriple Negative Breast Carcinomas.

Changavi AA, Shashikala A, Ramji AS - J Lab Physicians (2015 Jul-Dec)

Bottom Line: Among these, EGFR plays an important role and is associated with bad prognosis.P < 0.05 was considered significant.EGFR expression was inversely associated with ER status and showed strong association with necrosis and lymphocytic infiltrate, but not with pushing border and periductal elastosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Sree Siddhartha Medical College, Tumkur, Karnataka, India.

ABSTRACT

Introduction: The panel of markers used for molecular classification include estrogen receptors (ER), progesterone receptors (PR), human epidermal growth factor receptor (HER)-2/neu, p53, Bcl-2 and basal markers like cytokeratin 5/6 or epidermal growth factor receptor (EGFR). Among these, EGFR plays an important role and is associated with bad prognosis.

Aims and objectives: To study EGFR expression in triple negative breast carcinoma (TNBC) and non-TNBCs (NTNBCs).

Materials and methods: Fifty cases of breast carcinomas were classified and graded according to World Health Organization and Nottingham modification of Scarff-Bloom-Richardson (SBR) system, respectively. The age of the patients ranged from 28 to 69 years. Histological features such as necrosis, pushing borders, lymphocytic infiltrate and periductal elastosis were noted. The panel of markers used in our study included ER, PR, HER-2/neu and EGFR. EGFR expression was assessed based on membrane staining. Chi-square test was applied for statistical analysis to compare EGFR expression with hormonal status and prognostic factors. P < 0.05 was considered significant.

Results: The mean age was 49.8 ± 7.2 years. There were 44 (88%) infiltrating ductal carcinoma, 3 (6%) medullary carcinoma and 3 (6%) mucinous carcinoma. EGFR expression was common in young patients and was predominant in TNBC (89.47%), was also expressed in few cases of NTNBC. There was a positive correlation of EGFR expression (P = 0.03491) with a high grade. Medullary carcinomas were triple negative and strongly expressed EGFR. EGFR expression was inversely associated with ER status and showed strong association with necrosis and lymphocytic infiltrate, but not with pushing border and periductal elastosis.

Conclusion: EGFR is an important marker to stratify patients with breast cancer according to molecular classification. Its expression correlated positively with young age, higher SBR grade, necrosis, lymphocytic infiltrate and inversely with hormonal receptor expression.

No MeSH data available.


Related in: MedlinePlus

Epidermal growth factor receptor (membrane positivity) by immunohistochemistry (IHC) in triple negative breast carcinoma (IHC, ×40)
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Figure 1: Epidermal growth factor receptor (membrane positivity) by immunohistochemistry (IHC) in triple negative breast carcinoma (IHC, ×40)

Mentions: The comparison of SBR grade and EGFR expression are shown in Table 2. There was a positive correlation with Grade III and Grade II (P = 0.03491). EGFR expression was predominantly noted in 17/19 (89.47%) of triple negative type [Figure 1]. Nontriple negative tumors, 7/15 (46.67%) HER-2/neu positive carcinomas [Figure 2a–c] and 4/9 (44.44%) of luminal B (basoluminal type) carcinomas [Figure 3] also showed EGFR positivity. Out of 4 basoluminal cases, which expressed EGFR, one case (Grade II) showed 3+ positivity and other 3 were 2+. All luminal a type breast carcinomas were EGFR negative. In this study, we noted that EGFR expression was strong in triple negative tumors compared to other types.


Epidermal Growth Factor Receptor Expression in Triple Negative and Nontriple Negative Breast Carcinomas.

Changavi AA, Shashikala A, Ramji AS - J Lab Physicians (2015 Jul-Dec)

Epidermal growth factor receptor (membrane positivity) by immunohistochemistry (IHC) in triple negative breast carcinoma (IHC, ×40)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4559633&req=5

Figure 1: Epidermal growth factor receptor (membrane positivity) by immunohistochemistry (IHC) in triple negative breast carcinoma (IHC, ×40)
Mentions: The comparison of SBR grade and EGFR expression are shown in Table 2. There was a positive correlation with Grade III and Grade II (P = 0.03491). EGFR expression was predominantly noted in 17/19 (89.47%) of triple negative type [Figure 1]. Nontriple negative tumors, 7/15 (46.67%) HER-2/neu positive carcinomas [Figure 2a–c] and 4/9 (44.44%) of luminal B (basoluminal type) carcinomas [Figure 3] also showed EGFR positivity. Out of 4 basoluminal cases, which expressed EGFR, one case (Grade II) showed 3+ positivity and other 3 were 2+. All luminal a type breast carcinomas were EGFR negative. In this study, we noted that EGFR expression was strong in triple negative tumors compared to other types.

Bottom Line: Among these, EGFR plays an important role and is associated with bad prognosis.P < 0.05 was considered significant.EGFR expression was inversely associated with ER status and showed strong association with necrosis and lymphocytic infiltrate, but not with pushing border and periductal elastosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Sree Siddhartha Medical College, Tumkur, Karnataka, India.

ABSTRACT

Introduction: The panel of markers used for molecular classification include estrogen receptors (ER), progesterone receptors (PR), human epidermal growth factor receptor (HER)-2/neu, p53, Bcl-2 and basal markers like cytokeratin 5/6 or epidermal growth factor receptor (EGFR). Among these, EGFR plays an important role and is associated with bad prognosis.

Aims and objectives: To study EGFR expression in triple negative breast carcinoma (TNBC) and non-TNBCs (NTNBCs).

Materials and methods: Fifty cases of breast carcinomas were classified and graded according to World Health Organization and Nottingham modification of Scarff-Bloom-Richardson (SBR) system, respectively. The age of the patients ranged from 28 to 69 years. Histological features such as necrosis, pushing borders, lymphocytic infiltrate and periductal elastosis were noted. The panel of markers used in our study included ER, PR, HER-2/neu and EGFR. EGFR expression was assessed based on membrane staining. Chi-square test was applied for statistical analysis to compare EGFR expression with hormonal status and prognostic factors. P < 0.05 was considered significant.

Results: The mean age was 49.8 ± 7.2 years. There were 44 (88%) infiltrating ductal carcinoma, 3 (6%) medullary carcinoma and 3 (6%) mucinous carcinoma. EGFR expression was common in young patients and was predominant in TNBC (89.47%), was also expressed in few cases of NTNBC. There was a positive correlation of EGFR expression (P = 0.03491) with a high grade. Medullary carcinomas were triple negative and strongly expressed EGFR. EGFR expression was inversely associated with ER status and showed strong association with necrosis and lymphocytic infiltrate, but not with pushing border and periductal elastosis.

Conclusion: EGFR is an important marker to stratify patients with breast cancer according to molecular classification. Its expression correlated positively with young age, higher SBR grade, necrosis, lymphocytic infiltrate and inversely with hormonal receptor expression.

No MeSH data available.


Related in: MedlinePlus