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Protective role of murine norovirus against Pseudomonas aeruginosa acute pneumonia.

Thépaut M, Grandjean T, Hober D, Lobert PE, Bortolotti P, Faure K, Dessein R, Kipnis E, Guery B - Vet. Res. (2015)

Bottom Line: Nevertheless, the effects of MNV infection on biomedical research are still unclear.Here we report that co-infection with MNV increases survival of mice with Pseudomonas aeruginosa acute lung injury and decreases in vivo production of pro-inflammatory cytokines.Our results suggest that MNV infection can deeply modify the parameters studied in conventional models of infection and lead to false conclusions in experimental models.

View Article: PubMed Central - PubMed

Affiliation: EA 7366: Pseudomonas aeruginosa Host-Pathogen Translational Research Group, UDSL, Université Lille Nord de France, 59045, Lille, France. Marion.thepaut@gmail.com.

ABSTRACT
The murine norovirus (MNV) is a recently discovered mouse pathogen, representing the most common contaminant in laboratory mouse colonies. Nevertheless, the effects of MNV infection on biomedical research are still unclear. We tested the hypothesis that MNV infection could alter immune response in mice with acute lung infection. Here we report that co-infection with MNV increases survival of mice with Pseudomonas aeruginosa acute lung injury and decreases in vivo production of pro-inflammatory cytokines. Our results suggest that MNV infection can deeply modify the parameters studied in conventional models of infection and lead to false conclusions in experimental models.

No MeSH data available.


Related in: MedlinePlus

Survival analysis. WT mice were infected per os with 1.107 PFU of MNV or PBS (n = 10/group). A week later, mice were infected with intranasal instillation of 5.106 CFU of P. aeruginosa or PBS. Lethality was monitored for 96 h after P. aeruginosa infection. *, P < 0.05 for a comparison with the control PBS/PBS; **, P < 0.01 for a comparison with the control PBS/PBS; ***, P < 0.001 for a comparison with the control PBS/PBS; °P < 0.05 for a comparison with PBS/CHA.
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Fig1: Survival analysis. WT mice were infected per os with 1.107 PFU of MNV or PBS (n = 10/group). A week later, mice were infected with intranasal instillation of 5.106 CFU of P. aeruginosa or PBS. Lethality was monitored for 96 h after P. aeruginosa infection. *, P < 0.05 for a comparison with the control PBS/PBS; **, P < 0.01 for a comparison with the control PBS/PBS; ***, P < 0.001 for a comparison with the control PBS/PBS; °P < 0.05 for a comparison with PBS/CHA.

Mentions: To determine whether an established infection with MNV affected P. aeruginosa infection, survival was analysed for 96 h. After 7 days of colonisation with the virus, mice were infected by intranasal instillation of P. aeruginosa. No mortality was observed for two control groups PBS/PBS and MNV/PBS. A dose of 5.106 CFU of P. aeruginosa induced 100% lethality in the PBS/CHA group. Infection with MNV before P. aeruginosa was associated to a 40% survival (Figure 1).Figure 1


Protective role of murine norovirus against Pseudomonas aeruginosa acute pneumonia.

Thépaut M, Grandjean T, Hober D, Lobert PE, Bortolotti P, Faure K, Dessein R, Kipnis E, Guery B - Vet. Res. (2015)

Survival analysis. WT mice were infected per os with 1.107 PFU of MNV or PBS (n = 10/group). A week later, mice were infected with intranasal instillation of 5.106 CFU of P. aeruginosa or PBS. Lethality was monitored for 96 h after P. aeruginosa infection. *, P < 0.05 for a comparison with the control PBS/PBS; **, P < 0.01 for a comparison with the control PBS/PBS; ***, P < 0.001 for a comparison with the control PBS/PBS; °P < 0.05 for a comparison with PBS/CHA.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4558952&req=5

Fig1: Survival analysis. WT mice were infected per os with 1.107 PFU of MNV or PBS (n = 10/group). A week later, mice were infected with intranasal instillation of 5.106 CFU of P. aeruginosa or PBS. Lethality was monitored for 96 h after P. aeruginosa infection. *, P < 0.05 for a comparison with the control PBS/PBS; **, P < 0.01 for a comparison with the control PBS/PBS; ***, P < 0.001 for a comparison with the control PBS/PBS; °P < 0.05 for a comparison with PBS/CHA.
Mentions: To determine whether an established infection with MNV affected P. aeruginosa infection, survival was analysed for 96 h. After 7 days of colonisation with the virus, mice were infected by intranasal instillation of P. aeruginosa. No mortality was observed for two control groups PBS/PBS and MNV/PBS. A dose of 5.106 CFU of P. aeruginosa induced 100% lethality in the PBS/CHA group. Infection with MNV before P. aeruginosa was associated to a 40% survival (Figure 1).Figure 1

Bottom Line: Nevertheless, the effects of MNV infection on biomedical research are still unclear.Here we report that co-infection with MNV increases survival of mice with Pseudomonas aeruginosa acute lung injury and decreases in vivo production of pro-inflammatory cytokines.Our results suggest that MNV infection can deeply modify the parameters studied in conventional models of infection and lead to false conclusions in experimental models.

View Article: PubMed Central - PubMed

Affiliation: EA 7366: Pseudomonas aeruginosa Host-Pathogen Translational Research Group, UDSL, Université Lille Nord de France, 59045, Lille, France. Marion.thepaut@gmail.com.

ABSTRACT
The murine norovirus (MNV) is a recently discovered mouse pathogen, representing the most common contaminant in laboratory mouse colonies. Nevertheless, the effects of MNV infection on biomedical research are still unclear. We tested the hypothesis that MNV infection could alter immune response in mice with acute lung infection. Here we report that co-infection with MNV increases survival of mice with Pseudomonas aeruginosa acute lung injury and decreases in vivo production of pro-inflammatory cytokines. Our results suggest that MNV infection can deeply modify the parameters studied in conventional models of infection and lead to false conclusions in experimental models.

No MeSH data available.


Related in: MedlinePlus