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Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis.

Zhang Y, Zhao J, Wang X, Xu X, Pan W - Parasit Vectors (2015)

Bottom Line: Of the six antigens, SjSP-13, SjSP-160 and SjSP-216 were highly expressed in 21-day old young worms, while SjSP-23, SjSP-164 and SjSP-189 were highly expressed in eggs.However, in the rabbit model, only anti-SjSP-216 antibody showed a significant increase at this time point.This study strongly suggests that SjSP-216, a highly expressed gene in the young worms, could serve as a potential biomarker for the early immunodiagnosis of S. japonicum infections in vertebrate hosts.

View Article: PubMed Central - PubMed

Affiliation: Institute for Infectious Diseases and Vaccine Development, Tongji University School of Medicine, Shanghai, 200092, China. zyb053127@126.com.

ABSTRACT

Background: Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma japonicum infections. In this study, we evaluated these antigens as candidate biomarkers for the early diagnosis of schistosomiasis in mice and rabbits.

Methods: The transcriptional profiles of the six antigens (SjSP-13, SjSP-23, SjSP-160, SjSP-164, SjSP-189 and SjSP-216) at different developmental stages were analyzed by quantitative PCR. The recombinant proteins were expressed in E. coli and purified with nickel chelate affinity chromatography. We then developed recombinant protein-based ELISA kits to analyze the kinetics of antigen-specific antibodies during the course of infection in mice and rabbits. The early diagnostic validity of the candidate SjSP-216 was further evaluated in mice and rabbits infected with S. japonicum.

Results: Of the six antigens, SjSP-13, SjSP-160 and SjSP-216 were highly expressed in 21-day old young worms, while SjSP-23, SjSP-164 and SjSP-189 were highly expressed in eggs. In the mouse model, we detected a significant increase in antibodies against SjSP-13 and SjSP-216 at 3 weeks post-infection. However, in the rabbit model, only anti-SjSP-216 antibody showed a significant increase at this time point. We recorded 100 % diagnostic sensitivity and specificity of SjSP-216-based ELISA in both infected mice and rabbits, 3 weeks after infection.

Conclusions: This study strongly suggests that SjSP-216, a highly expressed gene in the young worms, could serve as a potential biomarker for the early immunodiagnosis of S. japonicum infections in vertebrate hosts.

No MeSH data available.


Related in: MedlinePlus

Kinetics of specific antibodies to the six antigens during the course of rabbit schistosomiasis. Sera were collected from three infected and uninfected rabbits at different time points. The antigen-specific antibody was analyzed by ELISA. The absorbance values were expressed as the mean ± standard error. a Changes in anti-SjSP-13 antibody. b Changes in anti-SjSP-23 antibody. c Changes in anti-SjSP-160 antibody. d Changes in anti-SjSP-164 antibody. e Changes in anti-SjSP-189 antibody. f Changes in anti-SjSP-216 antibody
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Fig3: Kinetics of specific antibodies to the six antigens during the course of rabbit schistosomiasis. Sera were collected from three infected and uninfected rabbits at different time points. The antigen-specific antibody was analyzed by ELISA. The absorbance values were expressed as the mean ± standard error. a Changes in anti-SjSP-13 antibody. b Changes in anti-SjSP-23 antibody. c Changes in anti-SjSP-160 antibody. d Changes in anti-SjSP-164 antibody. e Changes in anti-SjSP-189 antibody. f Changes in anti-SjSP-216 antibody

Mentions: We observed a significant increase in antibodies against SjSP-13, SjSP-23, SjSP-160 and SjSP-216 in the three infected rabbits 6 weeks post-infection (Fig. 3). There was no increase in antibodies against SjSP-164 and SjSP-189 during the course of infection (Fig. 3). As in the mouse host, antibodies against SjSP-216 increased significantly just 3 weeks after infection (Fig. 3f). Significant increases in antibodies against SjSP-13 did not occur until 5 weeks post-infection (Fig. 3a). Interestingly, antibodies against SjSP-23 could only be detected 6 weeks post-infection in both the mouse and the rabbit model. Hence, these results indicated only SjSP-216 could serve as a candidate for early diagnosis of schistosomiasis in rabbits.Fig. 3


Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis.

Zhang Y, Zhao J, Wang X, Xu X, Pan W - Parasit Vectors (2015)

Kinetics of specific antibodies to the six antigens during the course of rabbit schistosomiasis. Sera were collected from three infected and uninfected rabbits at different time points. The antigen-specific antibody was analyzed by ELISA. The absorbance values were expressed as the mean ± standard error. a Changes in anti-SjSP-13 antibody. b Changes in anti-SjSP-23 antibody. c Changes in anti-SjSP-160 antibody. d Changes in anti-SjSP-164 antibody. e Changes in anti-SjSP-189 antibody. f Changes in anti-SjSP-216 antibody
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4558877&req=5

Fig3: Kinetics of specific antibodies to the six antigens during the course of rabbit schistosomiasis. Sera were collected from three infected and uninfected rabbits at different time points. The antigen-specific antibody was analyzed by ELISA. The absorbance values were expressed as the mean ± standard error. a Changes in anti-SjSP-13 antibody. b Changes in anti-SjSP-23 antibody. c Changes in anti-SjSP-160 antibody. d Changes in anti-SjSP-164 antibody. e Changes in anti-SjSP-189 antibody. f Changes in anti-SjSP-216 antibody
Mentions: We observed a significant increase in antibodies against SjSP-13, SjSP-23, SjSP-160 and SjSP-216 in the three infected rabbits 6 weeks post-infection (Fig. 3). There was no increase in antibodies against SjSP-164 and SjSP-189 during the course of infection (Fig. 3). As in the mouse host, antibodies against SjSP-216 increased significantly just 3 weeks after infection (Fig. 3f). Significant increases in antibodies against SjSP-13 did not occur until 5 weeks post-infection (Fig. 3a). Interestingly, antibodies against SjSP-23 could only be detected 6 weeks post-infection in both the mouse and the rabbit model. Hence, these results indicated only SjSP-216 could serve as a candidate for early diagnosis of schistosomiasis in rabbits.Fig. 3

Bottom Line: Of the six antigens, SjSP-13, SjSP-160 and SjSP-216 were highly expressed in 21-day old young worms, while SjSP-23, SjSP-164 and SjSP-189 were highly expressed in eggs.However, in the rabbit model, only anti-SjSP-216 antibody showed a significant increase at this time point.This study strongly suggests that SjSP-216, a highly expressed gene in the young worms, could serve as a potential biomarker for the early immunodiagnosis of S. japonicum infections in vertebrate hosts.

View Article: PubMed Central - PubMed

Affiliation: Institute for Infectious Diseases and Vaccine Development, Tongji University School of Medicine, Shanghai, 200092, China. zyb053127@126.com.

ABSTRACT

Background: Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma japonicum infections. In this study, we evaluated these antigens as candidate biomarkers for the early diagnosis of schistosomiasis in mice and rabbits.

Methods: The transcriptional profiles of the six antigens (SjSP-13, SjSP-23, SjSP-160, SjSP-164, SjSP-189 and SjSP-216) at different developmental stages were analyzed by quantitative PCR. The recombinant proteins were expressed in E. coli and purified with nickel chelate affinity chromatography. We then developed recombinant protein-based ELISA kits to analyze the kinetics of antigen-specific antibodies during the course of infection in mice and rabbits. The early diagnostic validity of the candidate SjSP-216 was further evaluated in mice and rabbits infected with S. japonicum.

Results: Of the six antigens, SjSP-13, SjSP-160 and SjSP-216 were highly expressed in 21-day old young worms, while SjSP-23, SjSP-164 and SjSP-189 were highly expressed in eggs. In the mouse model, we detected a significant increase in antibodies against SjSP-13 and SjSP-216 at 3 weeks post-infection. However, in the rabbit model, only anti-SjSP-216 antibody showed a significant increase at this time point. We recorded 100 % diagnostic sensitivity and specificity of SjSP-216-based ELISA in both infected mice and rabbits, 3 weeks after infection.

Conclusions: This study strongly suggests that SjSP-216, a highly expressed gene in the young worms, could serve as a potential biomarker for the early immunodiagnosis of S. japonicum infections in vertebrate hosts.

No MeSH data available.


Related in: MedlinePlus