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New Insights into the Phylogeny and Gene Context Analysis of Binder of Sperm Proteins (BSPs).

Serrano E, Martínez AB, Arruga D, Pérez-Pé R, Sánchez-Ferrer Á, Muiño-Blanco T, Cebrián-Pérez JA - PLoS ONE (2015)

Bottom Line: Seminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability.BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla.However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica y Biología Molecular y Celular-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013, Zaragoza, Spain.

ABSTRACT
Seminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability. Among SP proteins, BSP (binder of sperm) proteins are the most studied, since they may be useful for the improvement of semen diluents, storage and subsequent fertilization results. However, an updated and detailed phylogenetic analysis of the BSP protein superfamily has not been carried out with all the sequences described in the main databases. The update view shows for the first time an equally distributed number of sequences between the three families: BSP, and their homologs 1 (BSPH1) and 2 (BSPH2). The BSP family is divided in four subfamilies, BSP1 subfamily being the predominant, followed by subfamilies BSP3, BSP5 and BSP2. BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla. However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade. This phylogenetic analysis, when combined with a gene context analysis, showed a completely new evolutionary scenario for the BSP superfamily of proteins with three defined different gene patterns, one for BSPs, one for BSPH1/BSPH2/ELSPBP1 and another one for BSPH1/BSPH2 without ELSPBP1. In addition, the study has permitted to define concise conserved blocks for each family (BSP, BSPH1 and BSPH2), which could be used for a more reliable assignment for the incoming sequences, for data curation of current databases, and for cloning new BSPs, as the one described in this paper, ram seminal vesicle 20 kDa protein (RSVP20, Ovis aries BSP5b).

No MeSH data available.


Conserved sequence blocks of two FN2 domains in Binder of Sperm Proteins.(A) Logo representation of 1FN2 domain (N-ter domain). (B) Logo representation of 2FN2 domain (C-ter domain). Symbols above sequences represent the secondary structure; springs represent helices and arrows represent β-strands. Orange lines connecting conserved cysteines correspond to disulphide bonds. Consensus sequences are shown below each conserved block. h stands for hydrophobic amino acid.
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pone.0137008.g002: Conserved sequence blocks of two FN2 domains in Binder of Sperm Proteins.(A) Logo representation of 1FN2 domain (N-ter domain). (B) Logo representation of 2FN2 domain (C-ter domain). Symbols above sequences represent the secondary structure; springs represent helices and arrows represent β-strands. Orange lines connecting conserved cysteines correspond to disulphide bonds. Consensus sequences are shown below each conserved block. h stands for hydrophobic amino acid.

Mentions: In order to fully understand the results described in Fig 1, a detailed study of the conserved sequence blocks was carried out using WebLogo3 [16] and ESPript [17] representations of the three different subfamilies (BSP, BSPH1 and BSPH2), and illustrated in base of RSVP20 sequence, corresponding to a non characterized BSP5 protein (S2 Fig). Nine conserved blocks were found (Fig 2), corresponding to two tandem FN2 domains with four blocks each and a linker block in between. Surprisingly, the C-ter FN2 (2FN2) domain is four amino acids longer than N-ter FN2 (1FN2) (Fig 2).


New Insights into the Phylogeny and Gene Context Analysis of Binder of Sperm Proteins (BSPs).

Serrano E, Martínez AB, Arruga D, Pérez-Pé R, Sánchez-Ferrer Á, Muiño-Blanco T, Cebrián-Pérez JA - PLoS ONE (2015)

Conserved sequence blocks of two FN2 domains in Binder of Sperm Proteins.(A) Logo representation of 1FN2 domain (N-ter domain). (B) Logo representation of 2FN2 domain (C-ter domain). Symbols above sequences represent the secondary structure; springs represent helices and arrows represent β-strands. Orange lines connecting conserved cysteines correspond to disulphide bonds. Consensus sequences are shown below each conserved block. h stands for hydrophobic amino acid.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557993&req=5

pone.0137008.g002: Conserved sequence blocks of two FN2 domains in Binder of Sperm Proteins.(A) Logo representation of 1FN2 domain (N-ter domain). (B) Logo representation of 2FN2 domain (C-ter domain). Symbols above sequences represent the secondary structure; springs represent helices and arrows represent β-strands. Orange lines connecting conserved cysteines correspond to disulphide bonds. Consensus sequences are shown below each conserved block. h stands for hydrophobic amino acid.
Mentions: In order to fully understand the results described in Fig 1, a detailed study of the conserved sequence blocks was carried out using WebLogo3 [16] and ESPript [17] representations of the three different subfamilies (BSP, BSPH1 and BSPH2), and illustrated in base of RSVP20 sequence, corresponding to a non characterized BSP5 protein (S2 Fig). Nine conserved blocks were found (Fig 2), corresponding to two tandem FN2 domains with four blocks each and a linker block in between. Surprisingly, the C-ter FN2 (2FN2) domain is four amino acids longer than N-ter FN2 (1FN2) (Fig 2).

Bottom Line: Seminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability.BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla.However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica y Biología Molecular y Celular-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013, Zaragoza, Spain.

ABSTRACT
Seminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability. Among SP proteins, BSP (binder of sperm) proteins are the most studied, since they may be useful for the improvement of semen diluents, storage and subsequent fertilization results. However, an updated and detailed phylogenetic analysis of the BSP protein superfamily has not been carried out with all the sequences described in the main databases. The update view shows for the first time an equally distributed number of sequences between the three families: BSP, and their homologs 1 (BSPH1) and 2 (BSPH2). The BSP family is divided in four subfamilies, BSP1 subfamily being the predominant, followed by subfamilies BSP3, BSP5 and BSP2. BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla. However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade. This phylogenetic analysis, when combined with a gene context analysis, showed a completely new evolutionary scenario for the BSP superfamily of proteins with three defined different gene patterns, one for BSPs, one for BSPH1/BSPH2/ELSPBP1 and another one for BSPH1/BSPH2 without ELSPBP1. In addition, the study has permitted to define concise conserved blocks for each family (BSP, BSPH1 and BSPH2), which could be used for a more reliable assignment for the incoming sequences, for data curation of current databases, and for cloning new BSPs, as the one described in this paper, ram seminal vesicle 20 kDa protein (RSVP20, Ovis aries BSP5b).

No MeSH data available.