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New Insights into the Phylogeny and Gene Context Analysis of Binder of Sperm Proteins (BSPs).

Serrano E, Martínez AB, Arruga D, Pérez-Pé R, Sánchez-Ferrer Á, Muiño-Blanco T, Cebrián-Pérez JA - PLoS ONE (2015)

Bottom Line: Seminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability.BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla.However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica y Biología Molecular y Celular-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013, Zaragoza, Spain.

ABSTRACT
Seminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability. Among SP proteins, BSP (binder of sperm) proteins are the most studied, since they may be useful for the improvement of semen diluents, storage and subsequent fertilization results. However, an updated and detailed phylogenetic analysis of the BSP protein superfamily has not been carried out with all the sequences described in the main databases. The update view shows for the first time an equally distributed number of sequences between the three families: BSP, and their homologs 1 (BSPH1) and 2 (BSPH2). The BSP family is divided in four subfamilies, BSP1 subfamily being the predominant, followed by subfamilies BSP3, BSP5 and BSP2. BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla. However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade. This phylogenetic analysis, when combined with a gene context analysis, showed a completely new evolutionary scenario for the BSP superfamily of proteins with three defined different gene patterns, one for BSPs, one for BSPH1/BSPH2/ELSPBP1 and another one for BSPH1/BSPH2 without ELSPBP1. In addition, the study has permitted to define concise conserved blocks for each family (BSP, BSPH1 and BSPH2), which could be used for a more reliable assignment for the incoming sequences, for data curation of current databases, and for cloning new BSPs, as the one described in this paper, ram seminal vesicle 20 kDa protein (RSVP20, Ovis aries BSP5b).

No MeSH data available.


Phylogenetic analysis of Binder of Sperm Proteins.BSP proteins are divided into the main subfamilies: BSP (green), BSPH1 (purple) and BSPH2 (hot pink). In addition, the BSP subfamily is composed of four different clades corresponding to BSP1 (olive green), BSP2 (yellow green), BSP3 (camouflage green) and BSP5 (light green). The structures behind each protein code represent domain composition: signal peptide (cyan), 1FN2 (red) and 2FN2 (fluorescent green). C-terminal section of the protein is the outer part of the domain representation. The neighbor-joining (NJ) tree was obtained from 1000 replicates. Bootstrap values are indicated in S1 Fig.
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pone.0137008.g001: Phylogenetic analysis of Binder of Sperm Proteins.BSP proteins are divided into the main subfamilies: BSP (green), BSPH1 (purple) and BSPH2 (hot pink). In addition, the BSP subfamily is composed of four different clades corresponding to BSP1 (olive green), BSP2 (yellow green), BSP3 (camouflage green) and BSP5 (light green). The structures behind each protein code represent domain composition: signal peptide (cyan), 1FN2 (red) and 2FN2 (fluorescent green). C-terminal section of the protein is the outer part of the domain representation. The neighbor-joining (NJ) tree was obtained from 1000 replicates. Bootstrap values are indicated in S1 Fig.

Mentions: The phylogenetic study was carried out using the unique 64 sequences found in the UniProt, Ensembl and NCBI databases. The tree obtained (Fig 1 and S1 Fig) shows an equally distributed number of sequences between the 3 families (BSP, BSPH1 and BSPH2). However, in BSP family, the predominant is BSP1 subfamily, followed by subfamilies BSP3 and BSP5. Of note are the only two equine sequences (Q70GG5 and F6XU34) found in the BSP2 subfamily (Fig 1, yellow green), which has evolved in parallel to the other two BSP1 sequences described in Equus caballus (Table 1, Fig 1). The BSP1 group (Fig 1, olive green) is basically formed by BSP proteins from leporidae (UniProt codes: G1U8W1 and G1U2M8), equidae (UniProt code: Q70GG5), suidae (UniProt code: P80964) and bovidae (UniProt codes: P02784 and B7VBV2), whereas the BSP3 group (Fig 1, camouflage green) is restricted to 4 bovinae sequences and a new O. aries BSP (UniProt code: UPI00029D7739). BSP5 clade (Fig 1, light green) is formed only by sequences of the bovinae and caprinae subfamilies, in which ram RSVP20 and RSVP22 [15] are located close to Bos taurus BSP5 (UniProt code: P81019), a new B. taurus BSP5 (UniProt code: L8HUS6) and also a new Ovis aries uncharacterized protein (UniProt code: W5PFH1), giving rise to a new defined clade, compared with previously described trees [13,14].


New Insights into the Phylogeny and Gene Context Analysis of Binder of Sperm Proteins (BSPs).

Serrano E, Martínez AB, Arruga D, Pérez-Pé R, Sánchez-Ferrer Á, Muiño-Blanco T, Cebrián-Pérez JA - PLoS ONE (2015)

Phylogenetic analysis of Binder of Sperm Proteins.BSP proteins are divided into the main subfamilies: BSP (green), BSPH1 (purple) and BSPH2 (hot pink). In addition, the BSP subfamily is composed of four different clades corresponding to BSP1 (olive green), BSP2 (yellow green), BSP3 (camouflage green) and BSP5 (light green). The structures behind each protein code represent domain composition: signal peptide (cyan), 1FN2 (red) and 2FN2 (fluorescent green). C-terminal section of the protein is the outer part of the domain representation. The neighbor-joining (NJ) tree was obtained from 1000 replicates. Bootstrap values are indicated in S1 Fig.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557993&req=5

pone.0137008.g001: Phylogenetic analysis of Binder of Sperm Proteins.BSP proteins are divided into the main subfamilies: BSP (green), BSPH1 (purple) and BSPH2 (hot pink). In addition, the BSP subfamily is composed of four different clades corresponding to BSP1 (olive green), BSP2 (yellow green), BSP3 (camouflage green) and BSP5 (light green). The structures behind each protein code represent domain composition: signal peptide (cyan), 1FN2 (red) and 2FN2 (fluorescent green). C-terminal section of the protein is the outer part of the domain representation. The neighbor-joining (NJ) tree was obtained from 1000 replicates. Bootstrap values are indicated in S1 Fig.
Mentions: The phylogenetic study was carried out using the unique 64 sequences found in the UniProt, Ensembl and NCBI databases. The tree obtained (Fig 1 and S1 Fig) shows an equally distributed number of sequences between the 3 families (BSP, BSPH1 and BSPH2). However, in BSP family, the predominant is BSP1 subfamily, followed by subfamilies BSP3 and BSP5. Of note are the only two equine sequences (Q70GG5 and F6XU34) found in the BSP2 subfamily (Fig 1, yellow green), which has evolved in parallel to the other two BSP1 sequences described in Equus caballus (Table 1, Fig 1). The BSP1 group (Fig 1, olive green) is basically formed by BSP proteins from leporidae (UniProt codes: G1U8W1 and G1U2M8), equidae (UniProt code: Q70GG5), suidae (UniProt code: P80964) and bovidae (UniProt codes: P02784 and B7VBV2), whereas the BSP3 group (Fig 1, camouflage green) is restricted to 4 bovinae sequences and a new O. aries BSP (UniProt code: UPI00029D7739). BSP5 clade (Fig 1, light green) is formed only by sequences of the bovinae and caprinae subfamilies, in which ram RSVP20 and RSVP22 [15] are located close to Bos taurus BSP5 (UniProt code: P81019), a new B. taurus BSP5 (UniProt code: L8HUS6) and also a new Ovis aries uncharacterized protein (UniProt code: W5PFH1), giving rise to a new defined clade, compared with previously described trees [13,14].

Bottom Line: Seminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability.BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla.However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica y Biología Molecular y Celular-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013, Zaragoza, Spain.

ABSTRACT
Seminal plasma (SP) proteins support the survival of spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, resulting in higher fertilizing ability. Among SP proteins, BSP (binder of sperm) proteins are the most studied, since they may be useful for the improvement of semen diluents, storage and subsequent fertilization results. However, an updated and detailed phylogenetic analysis of the BSP protein superfamily has not been carried out with all the sequences described in the main databases. The update view shows for the first time an equally distributed number of sequences between the three families: BSP, and their homologs 1 (BSPH1) and 2 (BSPH2). The BSP family is divided in four subfamilies, BSP1 subfamily being the predominant, followed by subfamilies BSP3, BSP5 and BSP2. BSPH proteins were found among placental mammals (Eutheria) belonging to the orders Proboscidea, Primates, Lagomorpha, Rodentia, Chiroptera, Perissodactyla and Cetartiodactyla. However, BSPH2 proteins were also found in the Scandentia order and Metatheria clade. This phylogenetic analysis, when combined with a gene context analysis, showed a completely new evolutionary scenario for the BSP superfamily of proteins with three defined different gene patterns, one for BSPs, one for BSPH1/BSPH2/ELSPBP1 and another one for BSPH1/BSPH2 without ELSPBP1. In addition, the study has permitted to define concise conserved blocks for each family (BSP, BSPH1 and BSPH2), which could be used for a more reliable assignment for the incoming sequences, for data curation of current databases, and for cloning new BSPs, as the one described in this paper, ram seminal vesicle 20 kDa protein (RSVP20, Ovis aries BSP5b).

No MeSH data available.