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Low Vitamin D Status Is Associated with Systemic and Gastrointestinal Inflammation in Dogs with a Chronic Enteropathy.

Titmarsh HF, Gow AG, Kilpatrick S, Cartwright JA, Milne EM, Philbey AW, Berry J, Handel I, Mellanby RJ - PLoS ONE (2015)

Bottom Line: Serum 25(OH)D concentrations, together with neutrophil, monocyte, eosinophil and lymphocyte counts, duodenal histopathology scores, serum IL-2, IL-6, IL-8 and TNFα concentrations and were measured in 39 dogs with histologically confirmed CE.A linear regression model examined the relationship between serum 25(OH)D status and measures of inflammation.Serum 25(OH)D concentrations were negatively associated with neutrophil and monocyte counts, duodenal histopathology scores and serum IL-2 and IL-8 concentrations.

View Article: PubMed Central - PubMed

Affiliation: Royal (Dick) School of Veterinary Studies and The Roslin Institute, The University of Edinburgh, Roslin, Midlothian, EH25 9RG, United Kingdom.

ABSTRACT

Introduction: Vitamin D deficiency, as assessed by serum concentrations of 25 hydroxyvitamin D (25(OH)D), has been linked to the development of over-zealous and inappropriate inflammation in humans. However, the relationship between vitamin D status and inflammation in dogs is ill-defined. Chronic enteropathies (CE) are frequently diagnosed in client owned dogs, have a wide range of serum 25(OH)D concentrations, and represent a spontaneous model in which to probe the relationship between vitamin D and inflammation. The hypothesis of this study was that vitamin D status would be negatively associated with systemic and gastrointestinal inflammation in dogs with a CE. The aim of this study was to examine the relationship between serum 25(OH)D concentrations and markers of systemic and gastrointestinal inflammation in a cohort of dogs with CE.

Methods and materials: Serum 25(OH)D concentrations, together with neutrophil, monocyte, eosinophil and lymphocyte counts, duodenal histopathology scores, serum IL-2, IL-6, IL-8 and TNFα concentrations and were measured in 39 dogs with histologically confirmed CE. A linear regression model examined the relationship between serum 25(OH)D status and measures of inflammation.

Results: Serum 25(OH)D concentrations were negatively associated with neutrophil and monocyte counts, duodenal histopathology scores and serum IL-2 and IL-8 concentrations. Dogs with low serum 25(OH)D concentrations typically had an inflammatory signature characterised by high monocyte and neutrophil numbers together with low lymphocyte numbers. There is a need to establish whether low vitamin D status is a cause or consequence of inflammation.

No MeSH data available.


Related in: MedlinePlus

Box and whisker plot of serum 25(OH)D concentrations by cluster.The box extends from 25% to 75% percentile with the median and the whiskers extend to limits of the data.
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pone.0137377.g002: Box and whisker plot of serum 25(OH)D concentrations by cluster.The box extends from 25% to 75% percentile with the median and the whiskers extend to limits of the data.

Mentions: Age and sex were not predictive of serum 25(OH)D concentrations in a linear regression model. The relationships between serum 25(OH)D concentrations and haematology, duodenal inflammation score and serum cytokine results are shown in Fig 1. Neutrophil and monocyte counts, duodenal histopathology score and serum IL-2 and IL-8 concentrations were negatively associated with 25(OH)D concentrations (Table 1). Three clusters were identified in the haematology data (Figs 2 and 3). There was a significant difference in 25(OH)D concentrations between the three clusters (p = 0.009). Post-test analysis revealed a significant difference between clusters 2 and 3 (p = 0.006).


Low Vitamin D Status Is Associated with Systemic and Gastrointestinal Inflammation in Dogs with a Chronic Enteropathy.

Titmarsh HF, Gow AG, Kilpatrick S, Cartwright JA, Milne EM, Philbey AW, Berry J, Handel I, Mellanby RJ - PLoS ONE (2015)

Box and whisker plot of serum 25(OH)D concentrations by cluster.The box extends from 25% to 75% percentile with the median and the whiskers extend to limits of the data.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557950&req=5

pone.0137377.g002: Box and whisker plot of serum 25(OH)D concentrations by cluster.The box extends from 25% to 75% percentile with the median and the whiskers extend to limits of the data.
Mentions: Age and sex were not predictive of serum 25(OH)D concentrations in a linear regression model. The relationships between serum 25(OH)D concentrations and haematology, duodenal inflammation score and serum cytokine results are shown in Fig 1. Neutrophil and monocyte counts, duodenal histopathology score and serum IL-2 and IL-8 concentrations were negatively associated with 25(OH)D concentrations (Table 1). Three clusters were identified in the haematology data (Figs 2 and 3). There was a significant difference in 25(OH)D concentrations between the three clusters (p = 0.009). Post-test analysis revealed a significant difference between clusters 2 and 3 (p = 0.006).

Bottom Line: Serum 25(OH)D concentrations, together with neutrophil, monocyte, eosinophil and lymphocyte counts, duodenal histopathology scores, serum IL-2, IL-6, IL-8 and TNFα concentrations and were measured in 39 dogs with histologically confirmed CE.A linear regression model examined the relationship between serum 25(OH)D status and measures of inflammation.Serum 25(OH)D concentrations were negatively associated with neutrophil and monocyte counts, duodenal histopathology scores and serum IL-2 and IL-8 concentrations.

View Article: PubMed Central - PubMed

Affiliation: Royal (Dick) School of Veterinary Studies and The Roslin Institute, The University of Edinburgh, Roslin, Midlothian, EH25 9RG, United Kingdom.

ABSTRACT

Introduction: Vitamin D deficiency, as assessed by serum concentrations of 25 hydroxyvitamin D (25(OH)D), has been linked to the development of over-zealous and inappropriate inflammation in humans. However, the relationship between vitamin D status and inflammation in dogs is ill-defined. Chronic enteropathies (CE) are frequently diagnosed in client owned dogs, have a wide range of serum 25(OH)D concentrations, and represent a spontaneous model in which to probe the relationship between vitamin D and inflammation. The hypothesis of this study was that vitamin D status would be negatively associated with systemic and gastrointestinal inflammation in dogs with a CE. The aim of this study was to examine the relationship between serum 25(OH)D concentrations and markers of systemic and gastrointestinal inflammation in a cohort of dogs with CE.

Methods and materials: Serum 25(OH)D concentrations, together with neutrophil, monocyte, eosinophil and lymphocyte counts, duodenal histopathology scores, serum IL-2, IL-6, IL-8 and TNFα concentrations and were measured in 39 dogs with histologically confirmed CE. A linear regression model examined the relationship between serum 25(OH)D status and measures of inflammation.

Results: Serum 25(OH)D concentrations were negatively associated with neutrophil and monocyte counts, duodenal histopathology scores and serum IL-2 and IL-8 concentrations. Dogs with low serum 25(OH)D concentrations typically had an inflammatory signature characterised by high monocyte and neutrophil numbers together with low lymphocyte numbers. There is a need to establish whether low vitamin D status is a cause or consequence of inflammation.

No MeSH data available.


Related in: MedlinePlus