Limits...
Bioconversion of Pinoresinol Diglucoside and Pinoresinol from Substrates in the Phenylpropanoid Pathway by Resting Cells of Phomopsis sp.XP-8.

Zhang Y, Shi J, Liu L, Gao Z, Che J, Shao D, Liu Y - PLoS ONE (2015)

Bottom Line: After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG.During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products.PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.

View Article: PubMed Central - PubMed

Affiliation: College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.

ABSTRACT
Pinoresinol diglucoside (PDG) and pinoresinol (Pin) are normally produced by plant cells via the phenylpropanoid pathway. This study reveals the existence of a related pathway in Phomopsis sp. XP-8, a PDG-producing fungal strain isolated from the bark of the Tu-chung tree (Eucommiaulmoides Oliv.). After addition of 0.15 g/L glucose to Phomopsis sp. XP-8, PDG and Pin formed when phenylalanine, tyrosine, leucine, cinnamic acid, and p-coumaric acid were used as the substrates respectively. No PDG formed in the absence of glucose, but Pin was detected after addition of all these substrates except leucine. In all systems in the presence of glucose, production of PDG and/or Pin and the accumulation of phenylalanine, cinnamic acid, or p-coumaric acid correlated directly with added substrate in a time- and substrate concentration- dependent manner. After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG. During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products. PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.

No MeSH data available.


Related in: MedlinePlus

Change of enzyme activities during reaction in the resting cell system with phenylalanine as the substrate.The data were obtained at 7 mmol/L phenylalanine in a, and after 40 h in b. The signals in the figure indicate enzymes 4CL (triangle), C4H (circle), and PAL (square)
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4557914&req=5

pone.0137066.g006: Change of enzyme activities during reaction in the resting cell system with phenylalanine as the substrate.The data were obtained at 7 mmol/L phenylalanine in a, and after 40 h in b. The signals in the figure indicate enzymes 4CL (triangle), C4H (circle), and PAL (square)

Mentions: When the activities of enzymes PAL, C4H, and 4CL were tracked during the bioconversion, it was found that each enzyme activity showed an increasing curve first, followed by a decrease during the bioconversion and also a decrease with further increase in Phe concentration (Fig 6). In all cases, PAL activity was maintained at a higher level than activities of 4CL and C4H, and C4H showed the lowest activity among the three enzymes, being consistent with a much higher accumulation of cinnamic acid than that of p-coumaric acid at the same condition (Fig 2).


Bioconversion of Pinoresinol Diglucoside and Pinoresinol from Substrates in the Phenylpropanoid Pathway by Resting Cells of Phomopsis sp.XP-8.

Zhang Y, Shi J, Liu L, Gao Z, Che J, Shao D, Liu Y - PLoS ONE (2015)

Change of enzyme activities during reaction in the resting cell system with phenylalanine as the substrate.The data were obtained at 7 mmol/L phenylalanine in a, and after 40 h in b. The signals in the figure indicate enzymes 4CL (triangle), C4H (circle), and PAL (square)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557914&req=5

pone.0137066.g006: Change of enzyme activities during reaction in the resting cell system with phenylalanine as the substrate.The data were obtained at 7 mmol/L phenylalanine in a, and after 40 h in b. The signals in the figure indicate enzymes 4CL (triangle), C4H (circle), and PAL (square)
Mentions: When the activities of enzymes PAL, C4H, and 4CL were tracked during the bioconversion, it was found that each enzyme activity showed an increasing curve first, followed by a decrease during the bioconversion and also a decrease with further increase in Phe concentration (Fig 6). In all cases, PAL activity was maintained at a higher level than activities of 4CL and C4H, and C4H showed the lowest activity among the three enzymes, being consistent with a much higher accumulation of cinnamic acid than that of p-coumaric acid at the same condition (Fig 2).

Bottom Line: After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG.During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products.PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.

View Article: PubMed Central - PubMed

Affiliation: College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.

ABSTRACT
Pinoresinol diglucoside (PDG) and pinoresinol (Pin) are normally produced by plant cells via the phenylpropanoid pathway. This study reveals the existence of a related pathway in Phomopsis sp. XP-8, a PDG-producing fungal strain isolated from the bark of the Tu-chung tree (Eucommiaulmoides Oliv.). After addition of 0.15 g/L glucose to Phomopsis sp. XP-8, PDG and Pin formed when phenylalanine, tyrosine, leucine, cinnamic acid, and p-coumaric acid were used as the substrates respectively. No PDG formed in the absence of glucose, but Pin was detected after addition of all these substrates except leucine. In all systems in the presence of glucose, production of PDG and/or Pin and the accumulation of phenylalanine, cinnamic acid, or p-coumaric acid correlated directly with added substrate in a time- and substrate concentration- dependent manner. After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG. During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products. PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.

No MeSH data available.


Related in: MedlinePlus