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Bioconversion of Pinoresinol Diglucoside and Pinoresinol from Substrates in the Phenylpropanoid Pathway by Resting Cells of Phomopsis sp.XP-8.

Zhang Y, Shi J, Liu L, Gao Z, Che J, Shao D, Liu Y - PLoS ONE (2015)

Bottom Line: After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG.During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products.PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.

View Article: PubMed Central - PubMed

Affiliation: College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.

ABSTRACT
Pinoresinol diglucoside (PDG) and pinoresinol (Pin) are normally produced by plant cells via the phenylpropanoid pathway. This study reveals the existence of a related pathway in Phomopsis sp. XP-8, a PDG-producing fungal strain isolated from the bark of the Tu-chung tree (Eucommiaulmoides Oliv.). After addition of 0.15 g/L glucose to Phomopsis sp. XP-8, PDG and Pin formed when phenylalanine, tyrosine, leucine, cinnamic acid, and p-coumaric acid were used as the substrates respectively. No PDG formed in the absence of glucose, but Pin was detected after addition of all these substrates except leucine. In all systems in the presence of glucose, production of PDG and/or Pin and the accumulation of phenylalanine, cinnamic acid, or p-coumaric acid correlated directly with added substrate in a time- and substrate concentration- dependent manner. After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG. During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products. PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.

No MeSH data available.


Related in: MedlinePlus

Effects of cinnamic acid additions on p-coumaric acid, PDG and Pin production.The signals in the figures indicate PDG (square), Pin (triangle) and p-coumaric acid (circle). The used condition is 1.5mmol/L cinnamic acid additions (a) and reaction time of 32 h (b).
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pone.0137066.g003: Effects of cinnamic acid additions on p-coumaric acid, PDG and Pin production.The signals in the figures indicate PDG (square), Pin (triangle) and p-coumaric acid (circle). The used condition is 1.5mmol/L cinnamic acid additions (a) and reaction time of 32 h (b).

Mentions: Production of p-coumaric acid, Pin and PDG were found when cinnamic acid (Fig 3) was used as the substrate together with 0.15 g/L glucose (as a glycosyl source). Production of PDG and Pin also occurred when p-coumaric acid (Fig 4) was used as the substrate together with 0.15 g/L glucose (as glycosyl source).


Bioconversion of Pinoresinol Diglucoside and Pinoresinol from Substrates in the Phenylpropanoid Pathway by Resting Cells of Phomopsis sp.XP-8.

Zhang Y, Shi J, Liu L, Gao Z, Che J, Shao D, Liu Y - PLoS ONE (2015)

Effects of cinnamic acid additions on p-coumaric acid, PDG and Pin production.The signals in the figures indicate PDG (square), Pin (triangle) and p-coumaric acid (circle). The used condition is 1.5mmol/L cinnamic acid additions (a) and reaction time of 32 h (b).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557914&req=5

pone.0137066.g003: Effects of cinnamic acid additions on p-coumaric acid, PDG and Pin production.The signals in the figures indicate PDG (square), Pin (triangle) and p-coumaric acid (circle). The used condition is 1.5mmol/L cinnamic acid additions (a) and reaction time of 32 h (b).
Mentions: Production of p-coumaric acid, Pin and PDG were found when cinnamic acid (Fig 3) was used as the substrate together with 0.15 g/L glucose (as a glycosyl source). Production of PDG and Pin also occurred when p-coumaric acid (Fig 4) was used as the substrate together with 0.15 g/L glucose (as glycosyl source).

Bottom Line: After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG.During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products.PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.

View Article: PubMed Central - PubMed

Affiliation: College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China.

ABSTRACT
Pinoresinol diglucoside (PDG) and pinoresinol (Pin) are normally produced by plant cells via the phenylpropanoid pathway. This study reveals the existence of a related pathway in Phomopsis sp. XP-8, a PDG-producing fungal strain isolated from the bark of the Tu-chung tree (Eucommiaulmoides Oliv.). After addition of 0.15 g/L glucose to Phomopsis sp. XP-8, PDG and Pin formed when phenylalanine, tyrosine, leucine, cinnamic acid, and p-coumaric acid were used as the substrates respectively. No PDG formed in the absence of glucose, but Pin was detected after addition of all these substrates except leucine. In all systems in the presence of glucose, production of PDG and/or Pin and the accumulation of phenylalanine, cinnamic acid, or p-coumaric acid correlated directly with added substrate in a time- and substrate concentration- dependent manner. After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p-coumaric acid, and finally to Pin or PDG. During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products. PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.

No MeSH data available.


Related in: MedlinePlus