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The nutrigenetic influence of the interaction between dietary vitamin E and TXN and COMT gene polymorphisms on waist circumference: a case control study.

Mansego ML, De Marco G, Ivorra C, Lopez-Izquierdo R, Morcillo S, Rojo-Martínez G, González-Albert V, Martinez F, Soriguer F, Martín-Escudero JC, Redon J, Chaves FJ - J Transl Med (2015)

Bottom Line: Logistic regression analysis revealed that the rs2301241 polymorphism in TXN and rs740603 in catechol-O-methyltransferase (COMT) were associated with waist circumference (WC) and AO.Moreover, these polymorphisms were more strongly associated with variations in WC in subjects with low vitamin E intakes.WC is associated both with dietary vitamin E intake and genetic variants of TXN and COMT suggesting that existence of a complex nutrigenetic pathway that involves regulation of AO.

View Article: PubMed Central - PubMed

Affiliation: CIBER Physiopathology of Obesity and Nutrition CIBERobn, CB06/03, Institute of Health Carlos III, c/Sinesio Delgado 6, 28029, Madrid, Spain. m.luisa.mansego@uv.es.

ABSTRACT

Background: Abdominal obesity (AO) is a common modifiable risk factor for certain non-communicable diseases associated with enhanced oxidative stress (OS). The objective of this work was to investigate whether the interaction between antioxidant vitamin intake and OS-related polymorphisms modulates gene-associated anthropometry in a Spanish population.

Methods: A total of 246 subjects with AO, and 492 age and gender matched non-AO subjects were included in the study. Anthropometric, biochemical, and OS parameters, and antioxidant dietary intake data were assessed using validated procedures. DNA from white blood cells was isolated and the genotype of seven polymorphisms from genes involved in OS (pro-oxidant and antioxidant) were analyzed using the SNPlex system. The effects of the c.-793T > C polymorphism on promoter activity and thus thioredoxin (TXN) activity were examined using reporter assays.

Results: The AO group had higher 8-Oxo-2'-deoxyguanosine levels and took in less vitamin A and vitamin E compared to the non-AO group. Logistic regression analysis revealed that the rs2301241 polymorphism in TXN and rs740603 in catechol-O-methyltransferase (COMT) were associated with waist circumference (WC) and AO. Moreover, these polymorphisms were more strongly associated with variations in WC in subjects with low vitamin E intakes. A promoter assay revealed that the T to C conversion at c.-793 (rs2301241) induced a more than two fold increase in reporter gene expression.

Conclusions: WC is associated both with dietary vitamin E intake and genetic variants of TXN and COMT suggesting that existence of a complex nutrigenetic pathway that involves regulation of AO.

No MeSH data available.


Related in: MedlinePlus

Analysis of the interaction between rs2301241 (a), rs740603 (b), and vitamin E intake on waist circumference. Data are the mean (±SEM). P values were assessed by one-way analysis of variance (ANOVA) and adjusted by age, gender, education level (<high school), hypertension, glucose, high-density lipoprotein cholesterol, triglycerides, 8-oxodeoxyguanosine, and alcohol consumption
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Fig2: Analysis of the interaction between rs2301241 (a), rs740603 (b), and vitamin E intake on waist circumference. Data are the mean (±SEM). P values were assessed by one-way analysis of variance (ANOVA) and adjusted by age, gender, education level (<high school), hypertension, glucose, high-density lipoprotein cholesterol, triglycerides, 8-oxodeoxyguanosine, and alcohol consumption

Mentions: With regard to rs2301241 (TXN), the TT-genotype subjects that consumed diets low in vitamin E had higher WCs than the C-allele carriers (Fig. 2a); the mean differences between these two genotypes were significantly different in this group (3.16 cm; p = 0.002, using a dominant [TC or CC] model). On the other hand, no significant genotype-based differences were found among subjects who consumed diets high in vitamin E. Carriers of the rs740603 (COMT) A allele who consumed diets low in vitamin E had a lower WC than homozygotes for the G allele (92.0 ± 12.1 vs. 95.12 ± 13.14 cm, p = 0.002; Fig. 2b), whereas WC was higher in GG genotype carriers who consumed diets low vitamin E compared to those with diets high in vitamin E (95.12 ± 13.14 vs. 92.4 ± 11.2 cm, p = 0.016). The interaction between SNPs and vitamin E intake was found to significantly influence WC in a dominant [GA or AA] model (p = 0.040). There were no significant interactions between vitamin A intake and SNPs (data no shown).Fig. 2


The nutrigenetic influence of the interaction between dietary vitamin E and TXN and COMT gene polymorphisms on waist circumference: a case control study.

Mansego ML, De Marco G, Ivorra C, Lopez-Izquierdo R, Morcillo S, Rojo-Martínez G, González-Albert V, Martinez F, Soriguer F, Martín-Escudero JC, Redon J, Chaves FJ - J Transl Med (2015)

Analysis of the interaction between rs2301241 (a), rs740603 (b), and vitamin E intake on waist circumference. Data are the mean (±SEM). P values were assessed by one-way analysis of variance (ANOVA) and adjusted by age, gender, education level (<high school), hypertension, glucose, high-density lipoprotein cholesterol, triglycerides, 8-oxodeoxyguanosine, and alcohol consumption
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4557824&req=5

Fig2: Analysis of the interaction between rs2301241 (a), rs740603 (b), and vitamin E intake on waist circumference. Data are the mean (±SEM). P values were assessed by one-way analysis of variance (ANOVA) and adjusted by age, gender, education level (<high school), hypertension, glucose, high-density lipoprotein cholesterol, triglycerides, 8-oxodeoxyguanosine, and alcohol consumption
Mentions: With regard to rs2301241 (TXN), the TT-genotype subjects that consumed diets low in vitamin E had higher WCs than the C-allele carriers (Fig. 2a); the mean differences between these two genotypes were significantly different in this group (3.16 cm; p = 0.002, using a dominant [TC or CC] model). On the other hand, no significant genotype-based differences were found among subjects who consumed diets high in vitamin E. Carriers of the rs740603 (COMT) A allele who consumed diets low in vitamin E had a lower WC than homozygotes for the G allele (92.0 ± 12.1 vs. 95.12 ± 13.14 cm, p = 0.002; Fig. 2b), whereas WC was higher in GG genotype carriers who consumed diets low vitamin E compared to those with diets high in vitamin E (95.12 ± 13.14 vs. 92.4 ± 11.2 cm, p = 0.016). The interaction between SNPs and vitamin E intake was found to significantly influence WC in a dominant [GA or AA] model (p = 0.040). There were no significant interactions between vitamin A intake and SNPs (data no shown).Fig. 2

Bottom Line: Logistic regression analysis revealed that the rs2301241 polymorphism in TXN and rs740603 in catechol-O-methyltransferase (COMT) were associated with waist circumference (WC) and AO.Moreover, these polymorphisms were more strongly associated with variations in WC in subjects with low vitamin E intakes.WC is associated both with dietary vitamin E intake and genetic variants of TXN and COMT suggesting that existence of a complex nutrigenetic pathway that involves regulation of AO.

View Article: PubMed Central - PubMed

Affiliation: CIBER Physiopathology of Obesity and Nutrition CIBERobn, CB06/03, Institute of Health Carlos III, c/Sinesio Delgado 6, 28029, Madrid, Spain. m.luisa.mansego@uv.es.

ABSTRACT

Background: Abdominal obesity (AO) is a common modifiable risk factor for certain non-communicable diseases associated with enhanced oxidative stress (OS). The objective of this work was to investigate whether the interaction between antioxidant vitamin intake and OS-related polymorphisms modulates gene-associated anthropometry in a Spanish population.

Methods: A total of 246 subjects with AO, and 492 age and gender matched non-AO subjects were included in the study. Anthropometric, biochemical, and OS parameters, and antioxidant dietary intake data were assessed using validated procedures. DNA from white blood cells was isolated and the genotype of seven polymorphisms from genes involved in OS (pro-oxidant and antioxidant) were analyzed using the SNPlex system. The effects of the c.-793T > C polymorphism on promoter activity and thus thioredoxin (TXN) activity were examined using reporter assays.

Results: The AO group had higher 8-Oxo-2'-deoxyguanosine levels and took in less vitamin A and vitamin E compared to the non-AO group. Logistic regression analysis revealed that the rs2301241 polymorphism in TXN and rs740603 in catechol-O-methyltransferase (COMT) were associated with waist circumference (WC) and AO. Moreover, these polymorphisms were more strongly associated with variations in WC in subjects with low vitamin E intakes. A promoter assay revealed that the T to C conversion at c.-793 (rs2301241) induced a more than two fold increase in reporter gene expression.

Conclusions: WC is associated both with dietary vitamin E intake and genetic variants of TXN and COMT suggesting that existence of a complex nutrigenetic pathway that involves regulation of AO.

No MeSH data available.


Related in: MedlinePlus